LOCUS       sequence-297233-        6242 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast Expression plasmid for SARS-CoV-2 ORF7a protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6242)
  TITLE     SARS-CoV-2 protein Yeast expression constructs, tagged, for protein 
            expression and/or purification
REFERENCE   2  (bases 1 to 6242)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4046227
FEATURES             Location/Qualifiers
     source          1..6242
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    25..142
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     433..456
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             837..854
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(1997..2015)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             2366..3169
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      3252..4234
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     terminator      4312..4341
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      4363..4950
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(4962..4989)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(5013..5204)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(5205..5996,5997..6065))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5828..5847
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6066..6168)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(6169..6200)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 gcggccgctt gctatattga agtacggatt agaagccgcc gagcgggcga cagccctccg
       61 acggaagact ctcctccgtg cgtcctcgtc ttcaccggtc gcgttcctga aacgcagatg
      121 tgcctcgcgc cgcactgctc cgaacaataa agattctaca atactagctt ttatggttat
      181 gaagaggaaa aattggcagt aacctggccc cacaaacctt caaattaacg aatcaaatta
      241 acaaccatag gatgataatg cgattagttt tttagcctta tttctggggt aattaatcag
      301 cgaagcgatg atttttgatc tattaacaga tatataaatg gaaaagctgc ataaccactt
      361 taactaatac aacattttca gtttgtatta cttcttattc aaatgtcata aaagtatcaa
      421 caaaaaattg ttaatatacc tctatacttt aacgtcaagg agaaaaaact ataatgaaaa
      481 ttattctttt cttggcactg ataacactcg ctacttgtga gctttatcac taccaagagt
      541 gtgttagagg tacaacagta cttttaaaag aaccttgctc ttctggaaca tacgagggca
      601 attcaccatt tcatcctcta gctgataaca aatttgcact gacttgcttt agcactcaat
      661 ttgcttttgc ttgtcctgac ggcgtaaaac acgtctatca gttacgtgcc agatcagttt
      721 cacctaaact gttcatcaga caagaggaag ttcaagaact ttactctcca atttttctta
      781 ttgttgcggc aatagtgttt ataacacttt gcttcacact caaaagaaag acagaactcg
      841 tgccgagagg ttctaaaatc gaggaaggta agctagtcat ttggataaat ggagataagg
      901 gttacaacgg tttggcagaa gtaggcaaga aatttgaaaa ggacacaggt atcaaggtga
      961 cggttgaaca tccagacaag ttagaagaaa aattccctca agttgctgct actggtgatg
     1021 gtcccgacat tattttctgg gcccacgatc gttttggtgg atatgctcag tcaggtttgc
     1081 tggctgagat caccccagac aaggccttcc aagataaatt gtacccattc acttgggatg
     1141 ctgtcagata caacggtaag cttatagcat atccaattgc tgttgaagcc ttatcgttga
     1201 tctacaataa ggacttgtta cctaacccac caaagacatg ggaagaaatt ccagctttgg
     1261 ataaagagct aaaggccaag ggaaaaagcg ctttgatgtt taacttacaa gaaccttatt
     1321 tcacctggcc attaatcgct gctgacggtg gttacgcatt taaatacgaa aacggtaagt
     1381 atgacattaa ggatgtcggc gttgataatg ctggtgcaaa ggctggtttg accttcttgg
     1441 ttgatttaat aaagaataaa cacatgaacg ctgacactga ctactctatc gccgaagccg
     1501 ctttcaacaa gggtgaaact gctatgacca taaatggtcc atgggcctgg agtaacattg
     1561 atacttctaa agtaaactat ggtgtcactg ttttgcctac attcaaaggc caaccatcca
     1621 agccctttgt cggtgttcta tccgctggca tcaatgctgc ttctccaaac aaggagctag
     1681 ctaaagaatt tttggaaaat tacttgttaa ctgacgaagg tttggaggcc gttaacaagg
     1741 acaagccatt gggtgctgtc gctttgaaat cttacgaaga agaattggct aaggatccca
     1801 gaattgccgc taccatggag aacgcccaaa agggtgaaat tatgccaaac atcccacaaa
     1861 tgtcagcctt ctggtacgca gttaggactg ccgtgattaa tgctgcttcc ggtagacaaa
     1921 ccgtcgacga agcattaaaa gatgctcaaa cgtaaacagg ccccttttcc tttgtcgata
     1981 tcatgtaatt agttatgtca cgcttacatt cacgccctcc tcccacatcc gctctaaccg
     2041 aaaaggaagg agttagacaa cctgaagtct aggtccctat ttattttttt taatagttat
     2101 gttagtatta agaacgttat ttatatttca aatttttctt ttttttctgt acaaagcggc
     2161 cgccgaacca ctagtgcact gcagtacacg gtttccttga aatttttttg attcggtaat
     2221 ctccgaacag aaggaagaac gaaggaagga gcacagactt agattggtat atatacgcat
     2281 atgtagtgtt gaagaaacat gaaattgccc agtattctta acccaactgc acagaacaaa
     2341 aacgtgcagg aaacgaagat aaatcatgtc gaaagctaca tataaggaac gtgctgctac
     2401 tcatcctagt cctgttgctg ccaagctatt taatatcatg cacgaaaagc aaacaaactt
     2461 gtgtgcttca ttggatgttc gtaccaccaa ggaattactg gagttagttg aagcattagg
     2521 tcccaaaatt tgtttactaa aaacacatgt ggatatcttg actgattttt ccatggaggg
     2581 cacagttaag ccgctaaagg cattatccgc caagtacaat tttttactct tcgaggacag
     2641 aaaatttgct gacattggta atacagtcaa attgcagtac tctgcgggtg tatacagaat
     2701 agcagaatgg gcagacatta cgaatgcaca cggtgtggtg ggcccaggta ttgttagcgg
     2761 tttgaagcag gcggcagaag aagtaacaaa ggaacctaga ggccttttga tgttagcaga
     2821 attgtcatgc aagggctccc tatctactgg agaatatact aagggtactg ttgacattgc
     2881 gaagagcgac aaagattttg ttatcggctt tattgctcaa agagacatgg gtggaagaga
     2941 tgaaggttac gattggttga ttatgacacc cggtgtgggt ttagatgaca agggagatgc
     3001 attgggtcaa cagtatagaa ccgtggatga tgtggtttct acaggatctg acattattat
     3061 tgttggaaga ggactatttg caaagggaag ggatgctaag gtagagggtg aacgttacag
     3121 aaaagcaggc tgggaagcat atttgagaag atgcggccag caaaactaaa aaactgtatt
     3181 ataagtaaat gcatgtatac taaactcaca aattagagct tcaatttaat tatatcagtt
     3241 attacccgag taacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag
     3301 cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga
     3361 aagcgctatt ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc
     3421 gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa
     3481 cgcgagagcg ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc
     3541 atcccgagag cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc
     3601 gctctataat gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa
     3661 ggctactttg gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt
     3721 actgattact agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat
     3781 tctataccga tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct
     3841 tcattggtca gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg
     3901 aaatgtttac attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt
     3961 ttttgtctaa agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg
     4021 caagttcaag gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata
     4081 tagcaaagag atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc
     4141 tcgttacagt ccggtgcgtt tttggttttt tgaaagtgcg tcatcagagc gcttttggtt
     4201 ttcaaaagcg ctctgaagtt cctatacttt ctagctagag aataggaact tcccgagcgg
     4261 ccgcgtgtta caagcttgac tccagcgtaa ctggactgca atcaactcac tggctcacct
     4321 tcacgggtgg gcctttcttc ggtagaaaat caaaggatct tcttgagatc ctttttttct
     4381 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     4441 ggatcaagag ctaccaactc tttttccgag gtaactggct tcagcagagc gcagatacca
     4501 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     4561 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     4621 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     4681 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     4741 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     4801 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg ggtaaacgcc
     4861 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcatcg atttttgtga
     4921 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcagaaag gcccacccga
     4981 aggtgagcca ggtgattaca tttgggccct catcagaggt tttcaccgtc atcaccgaaa
     5041 cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct
     5101 gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg
     5161 ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcacttacca atgcttaatc
     5221 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     5281 gtcgtgtaga taactacgat gcgggagggc ttaccatctg gccccagtgc tgcaatgata
     5341 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccgggagg
     5401 gccgagcgca gaagtgatcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     5461 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     5521 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     5581 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     5641 cctccgatcg ttgccagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     5701 ctgcataatt ctcttactgt catgccatcc gtgagatgct tttctgtgac tggtgagtac
     5761 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     5821 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     5881 tcttcggggc gtaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     5941 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     6001 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     6061 ctcattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt
     6121 gatcttattt cattatggtg aaagttggaa cctcttacgt gccgatcaag tcaaaagcct
     6181 ccggtcggag gcttttgact ttctgctatg gaggtcaggt atgatttaaa tggtcagtta
     6241 ga
//
