LOCUS       sequence-297236-        9079 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mPing Yeast Transposition Assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9079)
  AUTHORS   Hancock CN, Zhang F, Wessler SR
  TITLE     Transposition of the Tourist-MITE mPing in yeast: an assay that 
            retains key features of catalysis by the class 2 PIF/Harbinger 
            superfamily.
  JOURNAL   Mob DNA. 2010 Feb 1;1(1):5. doi: 10.1186/1759-8753-1-5.
  PUBMED    20226077
REFERENCE   2  (bases 1 to 9079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mob DNA."; 
            date: "2010-02-1"; volume: "1(1):5. doi"; pages: " 
            10.1186/1759-8753-1-5"
COMMENT     Sequence Label: pAG425 GAL Pong TPase L418A, L420A
FEATURES             Location/Qualifiers
     source          1..9079
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      69..1411
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    401..448
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        1438..1542
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1543..1611,1612..2403)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1761..1780)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2574..3162
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3063..3082
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3316..3333
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3450..3471
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3486..3491,3492..3509,3510..3516)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3524..3540
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3529..3551
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3548..3564
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3548..3564
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3583..3603
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3585..3603
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3627..4068
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    3627..3744
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     4039..4062
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     4083..4099
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4083..4099
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    4107..4131
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5631..5655)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5688..5704)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5689..5705)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      5707..5954
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(5723..5741)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(5972..5991)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5973..5991)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6001..6018)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6001..6017)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6010..6032)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6158..6613
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6245..6264)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6455..6476
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6914..7321
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             7322..8416
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(8910..8929)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9029..9051
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagtatga tccaatatca aaggaaatga tagcattgaa ggatgagact aatccaattg
      121 aggagtggca gcatatagaa cagctaaagg gtagtgctga aggaagcata cgataccccg
      181 catggaatgg gataatatca caggaggtac tagactacct ttcatcctac ataaatagac
      241 gcatataagt acgcatttaa gcataaacac gcactatgcc gttcttctca tgtatatata
      301 tatacaggca acacgcagat ataggtgcga cgtgaacagt gagctgtatg tgcgcagctc
      361 gcgttgcatt ttcggaagcg ctcgttttcg gaaacgcttt gaagttccta ttccgaagtt
      421 cctattctct agaaagtata ggaacttcag agcgcttttg aaaaccaaaa gcgctctgaa
      481 gacgcacttt caaaaaacca aaaacgcacc ggactgtaac gagctactaa aatattgcga
      541 ataccgcttc cacaaacatt gctcaaaagt atctctttgc tatatatctc tgtgctatat
      601 ccctatataa cctacccatc cacctttcgc tccttgaact tgcatctaaa ctcgacctct
      661 acatttttta tgtttatctc tagtattact ctttagacaa aaaaattgta gtaagaacta
      721 ttcatagagt gaatcgaaaa caatacgaaa atgtaaacat ttcctatacg tagtatatag
      781 agacaaaata gaagaaaccg ttcataattt tctgaccaat gaagaatcat caacgctatc
      841 actttctgtt cacaaagtat gcgcaatcca catcggtata gaatataatc ggggatgcct
      901 ttatcttgaa aaaatgcacc cgcagcttcg ctagtaatca gtaaacgcgg gaagtggagt
      961 caggcttttt ttatggaaga gaaaatagac accaaagtag ccttcttcta accttaacgg
     1021 acctacagtg caaaaagtta tcaagagact gcattataga gcgcacaaag gagaaaaaaa
     1081 gtaatctaag atgctttgtt agaaaaatag cgctctcggg atgcattttt gtagaacaaa
     1141 aaagaagtat agattctttg ttggtaaaat agcgctctcg cgttgcattt ctgttctgta
     1201 aaaatgcagc tcagattctt tgtttgaaaa attagcgctc tcgcgttgca tttttgtttt
     1261 acaaaaatga agcacagatt cttcgttggt aaaatagcgc tttcgcgttg catttctgtt
     1321 ctgtaaaaat gcagctcaga ttctttgttt gaaaaattag cgctctcgcg ttgcattttt
     1381 gttctacaaa atgaagcaca gatgcttcgt tcaggtggca cttttcgggg aaatgtgcgc
     1441 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     1501 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     1561 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     1621 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     1681 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     1741 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     1801 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     1861 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     1921 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     1981 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     2041 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     2101 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     2161 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     2221 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     2281 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     2341 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     2401 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     2461 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     2521 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     2581 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     2641 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     2701 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     2761 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     2821 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     2881 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2941 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     3001 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     3061 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     3121 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     3181 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     3241 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     3301 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     3361 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     3421 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     3481 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     3541 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
     3601 agggaacaaa agctggagct ctagtacgga ttagaagccg ccgagcgggt gacagccctc
     3661 cgaaggaaga ctctcctccg tgcgtcctcg tcttcaccgg tcgcgttcct gaaacgcaga
     3721 tgtgcctcgc gccgcactgc tccgaacaat aaagattcta caatactagc ttttatggtt
     3781 atgaagagga aaaattggca gtaacctggc cccacaaacc ttcaaatgaa cgaatcaaat
     3841 taacaaccat aggatgataa tgcgattagt tttttagcct tatttctggg gtaattaatc
     3901 agcgaagcga tgatttttga tctattaaca gatatataaa tgcaaaaact gcataaccac
     3961 tttaactaat actttcaaca ttttcggttt gtattacttc ttattcaaat gtaataaaag
     4021 tatcaacaaa aaattgttaa tatacctcta tactttaacg tcaaggagaa aaaaccccgg
     4081 attctagaac tagtggatcc cccatcacaa gtttgtacaa aaaagcaggc tccgcggccg
     4141 cccccttcac catgcagagt ttagccatct ctctactcct ctcagaaact cattccctct
     4201 tttctcatac gaagacctcc tcccttttat ctttactgtt tctctcttct tcaaagatgt
     4261 ctgagcaaaa tactgatgga agtcaagttc cagtgaactt gttggatgag ttcctggctg
     4321 aggatgagat catagatgat cttctcactg aagccacggt ggtagtacag tccactatag
     4381 aaggtcttca aaacgaggct tctgaccatc gacatcatcc gaggaagcac atcaagaggc
     4441 cacgagagga agcacatcag caactagtga atgattactt ttcagaaaat cctctttacc
     4501 cttccaaaat ttttcgtcga agatttcgta tgtctaggcc actttttctt cgcatcgttg
     4561 aggcattagg ccagtggtca gtgtatttca cacaaagggt ggatgctgtt aatcggaaag
     4621 gactcagtcc actgcaaaag tgtactgcag ctattcgcca gttggctact ggtagtggcg
     4681 cagatgaact agatgaatat ctgaagatag gagagactac agcaatggag gcaatgaaga
     4741 attttgtcaa aggtcttcaa gatgtgtttg gtgagaggta tcttaggcgc cccaccatgg
     4801 aagataccga acggcttctc caacttggtg agaaacgtgg ttttcctgga atgttcggca
     4861 gcattgactg catgcactgg cattgggaaa gatgcccagt agcatggaag ggtcagttca
     4921 ctcgtggaga tcagaaagtg ccaaccctga ttcttgaggc tgtggcatcg catgatcttt
     4981 ggatttggca tgcatttttt ggagcagcgg gttccaacaa tgatatcaat gtattgaacc
     5041 aatctactgt atttatcaag gagctcaaag gacaagctcc tagagtccag tacatggtaa
     5101 atgggaatca atacaatact gggtattttc ttgctgatgg aatctaccct gaatgggcag
     5161 tgtttgttaa gtcaatacga ctcccaaaca ctgaaaagga gaaattgtat gcagatatgc
     5221 aagaaggggc aagaaaagat atcgagagag cctttggtgt attgcagcga agattttgca
     5281 tcttaaaacg accagctcgt ctatatgatc gaggtgtact gcgagatgtt gttctagctt
     5341 gcatcatact tcacaatatg atagttgaag atgagaagga aaccagaatt attgaagaag
     5401 atgcagatgc aaatgtgcct cctagttcat caaccgttca ggaacctgag ttctctcctg
     5461 aacagaacac accatttgat agagttttag aaaaagatat ttctatccga gatcgagcgg
     5521 ctcataaccg acttaagaaa gatttggtgg aacacatttg gaataagttt ggtggtgctg
     5581 cacatagaac tggaaattga gcggccgcat actgaagggt gggcgcgccg acccagcttt
     5641 cttgtacaaa gtggtgatgg gctgcaggaa ttcgatatca agcttatcga taccgtcgac
     5701 ctcgagtcat gtaattagtt atgtcacgct tacattcacg ccctcccccc acatccgctc
     5761 taaccgaaaa ggaaggagtt agacaacctg aagtctaggt ccctatttat ttttttatag
     5821 ttatgttagt attaagaacg ttatttatat ttcaaatttt tctttttttt ctgtacagac
     5881 gcgtgtacgc atgtaacatt atactgaaaa ccttgcttga gaaggttttg ggacgctcga
     5941 aggctttaat ttgcggccgg tacccaattc gccctatagt gagtcgtatt acgcgcgctc
     6001 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     6061 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     6121 cccttcccaa cagttgcgca gcctgaatgg cgaatggacg cgccctgtag cggcgcatta
     6181 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     6241 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     6301 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     6361 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     6421 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     6481 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     6541 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     6601 acgcttacaa tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     6661 gcatatcgac cggtcgagga gaacttctag tatatctaca tacctaatat tattgcctta
     6721 ttaaaaatgg aatcccaaca attacatcaa aatccacatt ctcttcaaaa tcaattgtcc
     6781 tgtacttcct tgttcatgtg tgttcaaaaa cgttatattt ataggataat tatactctat
     6841 ttctcaacaa gtaattggtt gtttggccga gcggtctaag gcgcctgatt caagaaatat
     6901 cttgaccgca gttaactgtg ggaatactca ggtatcgtaa gatgcaagag ttcgaatctc
     6961 ttagcaacca ttattttttt cctcaacata acgagaacac acaggggcgc tatcgcacag
     7021 aatcaaattc gatgactgga aattttttgt taatttcaga ggtcgcctga cgcatatacc
     7081 tttttcaact gaaaaattgg gagaaaaagg aaaggtgaga gcgccggaac cggcttttca
     7141 tatagaatag agaagcgttc atgactaaat gcttgcatca caatacttga agttgacaat
     7201 attatttaag gacctattgt tttttccaat aggtggttag caatcgtctt actttctaac
     7261 ttttcttacc ttttacattt cagcaatata tatatatata tttcaaggat ataccattct
     7321 aatgtctgcc cctaagaaga tcgtcgtttt gccaggtgac cacgttggtc aagaaatcac
     7381 agccgaagcc attaaggttc ttaaagctat ttctgatgtt cgttccaatg tcaagttcga
     7441 tttcgaaaat catttaattg gtggtgctgc tatcgatgct acaggtgttc cacttccaga
     7501 tgaggcgctg gaagcctcca agaaggctga tgccgttttg ttaggtgctg tgggtggtcc
     7561 taaatggggt accggtagtg ttagacctga acaaggttta ctaaaaatcc gtaaagaact
     7621 tcaattgtac gccaacttaa gaccatgtaa ctttgcatcc gactctcttt tagacttatc
     7681 tccaatcaag ccacaatttg ctaaaggtac tgacttcgtt gttgtcagag aattagtggg
     7741 aggtatttac tttggtaaga gaaaggaaga cgatggtgat ggtgtcgctt gggatagtga
     7801 acaatacacc gttccagaag tgcaaagaat cacaagaatg gccgctttca tggccctaca
     7861 acatgagcca ccattgccta tttggtcctt ggataaagct aatgttttgg cctcttcaag
     7921 attatggaga aaaactgtgg aggaaaccat caagaacgaa ttccctacat tgaaggttca
     7981 acatcaattg attgattctg ccgccatgat cctagttaag aacccaaccc acctaaatgg
     8041 tattataatc accagcaaca tgtttggtga tatcatctcc gatgaagcct ccgttatccc
     8101 aggttccttg ggtttgttgc catctgcgtc cttggcctct ttgccagaca agaacaccgc
     8161 atttggtttg tacgaaccat gccacggttc tgctccagat ttgccaaaga ataaggtcaa
     8221 ccctatcgcc actatcttgt ctgctgcaat gatgttgaaa ttgtcattga acttgcctga
     8281 agaaggtaag gccattgaag atgcagttaa aaaggttttg gatgcaggta tcagaactgg
     8341 tgatttaggt ggttccaaca gtaccaccga agtcggtgat gctgtcgccg aagaagttaa
     8401 gaaaatcctt gcttaaaaag attctctttt tttatgatat ttgtacataa actttataaa
     8461 tgaaattcat aatagaaacg acacgaaatt acaaaatgga atatgttcat agggtagacg
     8521 aaactatata cgcaatctac atacatttat caagaaggag aaaaaggagg atgtaaagga
     8581 atacaggtaa gcaaattgat actaatggct caacgtgata aggaaaaaga attgcacttt
     8641 aacattaata ttgacaagga ggagggcacc acacaaaaag ttaggtgtaa cagaaaatca
     8701 tgaaactatg attcctaatt tatatattgg aggattttct ctaaaaaaaa aaaaatacaa
     8761 caaataaaaa acactcaatg acctgaccat ttgatggagt ttaagtcaat accttcttga
     8821 accatttccc ataatggtga aagttccctc aagaatttta ctctgtcaga aacggcctta
     8881 acgacgtagt cgatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     8941 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     9001 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     9061 tcatcaccga aacgcgcga
//
