LOCUS       sequence-297238-        6746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast Expression plasmid for SARS-CoV-2 nsp6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6746)
  TITLE     SARS-CoV-2 protein Yeast expression constructs, tagged, for protein 
            expression and/or purification
REFERENCE   2  (bases 1 to 6746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4046341
FEATURES             Location/Qualifiers
     source          1..6746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        25..466
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    25..142
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     437..460
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             1345..1362
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(2505..2523)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     rep_origin      3756..4738
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     terminator      4816..4845
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      4867..5454
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(5466..5493)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(5517..5708)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(5709..6500,6501..6569))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6332..6351
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6570..6672)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(6673..6704)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 gcggccgctt gctatattga agtacggatt agaagccgcc gagcgggcga cagccctccg
       61 acggaagact ctcctccgtg cgtcctcgtc ttcaccggtc gcgttcctga aacgcagatg
      121 tgcctcgcgc cgcactgctc cgaacaataa agattctaca atactagctt ttatggttat
      181 gaagaggaaa aattggcagt aacctggccc cacaaacctt caaattaacg aatcaaatta
      241 acaaccatag gatgataatg cgattagttt tttagcctta tttctggggt aattaatcag
      301 cgaagcgatg atttttgatc tattaacaga tatataaatg gaaaagctgc ataaccactt
      361 taactaatac tttcaacatt ttcagtttgt attacttctt attcaaatgt cataaaagta
      421 tcaacaaaaa attgttaata tacctctata ctttaacgtc aaggagaaaa aactataatg
      481 gcagtgaaaa gaacaatcaa gggtacacac cactggttgt tactcacaat tttgacttca
      541 cttttagttt tagtccagag tactcaatgg tctttgttct tttttttgta tgaaaatgcc
      601 tttttacctt ttgctatggg tattattgct atgtctgctt ttgcaatgat gtttgtcaaa
      661 cataagcatg catttctctg tttgtttttg ttaccttctc ttgccactgt agcttatttt
      721 aatatggtct atatgcctgc tagttgggtg atgcgtatta tgacatggtt ggatatggtt
      781 gatactagtt tgtctggttt taagctaaaa gactgtgtta tgtatgcatc agctgtagtg
      841 ttactaatcc ttatgacagc aagaactgtg tatgatgatg gtgctaggag agtgtggaca
      901 cttatgaatg tcttgacact cgtttataaa gtttattatg gtaatgcttt agatcaagcc
      961 atttccatgt gggctcttat aatctctgtt acttctaact actcaggtgt agttacaact
     1021 gtcatgtttt tggccagagg tattgttttt atgtgtgttg agtattgccc tattttcttc
     1081 ataactggta atacacttca gtgtataatg ctagtttatt gtttcttagg ctatttttgt
     1141 acttgttact ttggcctctt ttgtttactc aaccgctact ttagactgac tcttggtgtt
     1201 tatgattact tagtttctac acaggagttt agatatatga attcacaggg actactccca
     1261 cccaagaata gcatagatgc cttcaaactc aacattaaat tgttgggtgt tggtggcaaa
     1321 ccttgtatca aagtagccac tgtactcgtg ccgagaggtt ctaaaatcga ggaaggtaag
     1381 ctagtcattt ggataaatgg agataagggt tacaacggtt tggcagaagt aggcaagaaa
     1441 tttgaaaagg acacaggtat caaggtgacg gttgaacatc cagacaagtt agaagaaaaa
     1501 ttccctcaag ttgctgctac tggtgatggt cccgacatta ttttctgggc ccacgatcgt
     1561 tttggtggat atgctcagtc aggtttgctg gctgagatca ccccagacaa ggccttccaa
     1621 gataaattgt acccattcac ttgggatgct gtcagataca acggtaagct tatagcatat
     1681 ccaattgctg ttgaagcctt atcgttgatc tacaataagg acttgttacc taacccacca
     1741 aagacatggg aagaaattcc agctttggat aaagagctaa aggccaaggg aaaaagcgct
     1801 ttgatgttta acttacaaga accttatttc acctggccat taatcgctgc tgacggtggt
     1861 tacgcattta aatacgaaaa cggtaagtat gacattaagg atgtcggcgt tgataatgct
     1921 ggtgcaaagg ctggtttgac cttcttggtt gatttaataa agaataaaca catgaacgct
     1981 gacactgact actctatcgc cgaagccgct ttcaacaagg gtgaaactgc tatgaccata
     2041 aatggtccat gggcctggag taacattgat acttctaaag taaactatgg tgtcactgtt
     2101 ttgcctacat tcaaaggcca accatccaag ccctttgtcg gtgttctatc cgctggcatc
     2161 aatgctgctt ctccaaacaa ggagctagct aaagaatttt tggaaaatta cttgttaact
     2221 gacgaaggtt tggaggccgt taacaaggac aagccattgg gtgctgtcgc tttgaaatct
     2281 tacgaagaag aattggctaa ggatcccaga attgccgcta ccatggagaa cgcccaaaag
     2341 ggtgaaatta tgccaaacat cccacaaatg tcagccttct ggtacgcagt taggactgcc
     2401 gtgattaatg ctgcttccgg tagacaaacc gtcgacgaag cattaaaaga tgctcaaacg
     2461 taaacaggcc ccttttcctt tgtcgatatc atgtaattag ttatgtcacg cttacattca
     2521 cgccctcctc ccacatccgc tctaaccgaa aaggaaggag ttagacaacc tgaagtctag
     2581 gtccctattt atttttttta atagttatgt tagtattaag aacgttattt atatttcaaa
     2641 tttttctttt ttttctgtac aaagcggccg ccgaaccact agtgcactgc agtacacggt
     2701 ttccttgaaa tttttttgat tcggtaatct ccgaacagaa ggaagaacga aggaaggagc
     2761 acagacttag attggtatat atacgcatat gtagtgttga agaaacatga aattgcccag
     2821 tattcttaac ccaactgcac agaacaaaaa cgtgcaggaa acgaagataa atcatgtcga
     2881 aagctacata taaggaacgt gctgctactc atcctagtcc tgttgctgcc aagctattta
     2941 atatcatgca cgaaaagcaa acaaacttgt gtgcttcatt ggatgttcgt accaccaagg
     3001 aattactgga gttagttgaa gcattaggtc ccaaaatttg tttactaaaa acacatgtgg
     3061 atatcttgac tgatttttcc atggagggca cagttaagcc gctaaaggca ttatccgcca
     3121 agtacaattt tttactcttc gcagaaaatt tgctgacatt ggtaatacag tcaaattgca
     3181 gtactctgcg ggtgtataca gaatagcaga atgggcagac attacgaatg cacacggtgt
     3241 ggtgggccca ggtattgtta gcggtttgaa gcaggcggca gaagaagtaa caaaggaacc
     3301 tagaggcctt ttgatgttag cagaattgtc atgcaagggc tccctatcta ctggagaata
     3361 tactaagggt actgttgaca ttgcgaagag cgacaaagat tttgttatcg gctttattgc
     3421 tcaaagagac atgggtggaa gagatgaagg ttacgattgg ttgattatga cacccggtgt
     3481 gggtttagat gacaagggag atgcattggg tcaacagtat agaaccgtgg atgatgtggt
     3541 ttctacagga tctgacatta ttattgttgg aagaggacta tttgcaaagg gaagggatgc
     3601 taaggtagag ggtgaacgtt acagaaaagc aggctgggaa gcatatttga gaagatgcgg
     3661 ccagcaaaac taaaaaactg tattataagt aaatgcatgt atactaaact cacaaattag
     3721 agcttcaatt taattatatc agttattacc cgagtaacga agcatctgtg cttcattttg
     3781 tagaacaaaa atgcaacgcg agagcgctaa tttttcaaac aaagaatctg agctgcattt
     3841 ttacagaaca gaaatgcaac gcgaaagcgc tattttacca acgaagaatc tgtgcttcat
     3901 ttttgtaaaa caaaaatgca acgcgagagc gctaattttt caaacaaaga atctgagctg
     3961 catttttaca gaacagaaat gcaacgcgag agcgctattt taccaacaaa gaatctatac
     4021 ttcttttttg ttctacaaaa atgcatcccg agagcgctat ttttctaaca aagcatctta
     4081 gattactttt tttctccttt gtgcgctcta taatgcagtc tcttgataac tttttgcact
     4141 gtaggtccgt taaggttaga agaaggctac tttggtgtct attttctctt ccataaaaaa
     4201 agcctgactc cacttcccgc gtttactgat tactagcgaa gctgcgggtg cattttttca
     4261 agataaaggc atccccgatt atattctata ccgatgtgga ttgcgcatac tttgtgaaca
     4321 gaaagtgata gcgttgatga ttcttcattg gtcagaaaat tatgaacggt ttcttctatt
     4381 ttgtctctat atactacgta taggaaatgt ttacattttc gtattgtttt cgattcactc
     4441 tatgaatagt tcttactaca atttttttgt ctaaagagta atactagaga taaacataaa
     4501 aaatgtagag gtcgagttta gatgcaagtt caaggagcga aaggtggatg ggtaggttat
     4561 atagggatat agcacagaga tatatagcaa agagatactt ttgagcaatg tttgtggaag
     4621 cggtattcgc aatattttag tagctcgtta cagtccggtg cgtttttggt tttttgaaag
     4681 tgcgtcatca gagcgctttt ggttttcaaa agcgctctga agttcctata ctttctagct
     4741 agagaatagg aacttcccga gcggccgcgt gttacaagct tgactccagc gtaactggac
     4801 tgcaatcaac tcactggctc accttcacgg gtgggccttt cttcggtaga aaatcaaagg
     4861 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     4921 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaggtaact
     4981 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     5041 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     5101 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     5161 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     5221 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     5281 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     5341 agggagcttc caggggtaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     5401 tgacttgagc atcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     5461 agcaacgcag aaaggcccac ccgaaggtga gccaggtgat tacatttggg ccctcatcag
     5521 aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg
     5581 tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc
     5641 agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt
     5701 ttggtcactt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     5761 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatgcggga gggcttacca
     5821 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     5881 gcaataaacc agccagccgg gagggccgag cgcagaagtg atcctgcaac tttatccgcc
     5941 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     6001 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     6061 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     6121 aaaaaagcgg ttagctcctt cggtcctccg atcgttgcca gaagtaagtt ggccgcagtg
     6181 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtgaga
     6241 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     6301 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     6361 aaagtgctca tcattggaaa acgttcttcg gggcgtaaac tctcaaggat cttaccgctg
     6421 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     6481 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     6541 agggcgacac ggaaatgttg aatactcatt ttagcttcct tagctcctga aaatctcgat
     6601 aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt
     6661 acgtgccgat caagtcaaaa gcctccggtc ggaggctttt gactttctgc tatggaggtc
     6721 aggtatgatt taaatggtca gttaga
//
