LOCUS       sequence-297240-        8281 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mPing Yeast Transposition Assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8281)
  AUTHORS   Hancock CN, Zhang F, Wessler SR
  TITLE     Transposition of the Tourist-MITE mPing in yeast: an assay that 
            retains key features of catalysis by the class 2 PIF/Harbinger 
            superfamily.
  JOURNAL   Mob DNA. 2010 Feb 1;1(1):5. doi: 10.1186/1759-8753-1-5.
  PUBMED    20226077
REFERENCE   2  (bases 1 to 8281)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mob DNA."; 
            date: "2010-02-1"; volume: "1(1):5. doi"; pages: " 
            10.1186/1759-8753-1-5"
COMMENT     Sequence Label: pWL89A mPing
FEATURES             Location/Qualifiers
     source          1..8281
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        276..651
                     /gene="S. cerevisiae ADE2"
                     /label=ADE2 promoter
     primer_bind     complement(2975..2992)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2975..2991)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2984..3006)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    3748..4910
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN4 centromere fused to the 
                     autonomously replicating sequence ARS1/ARS416"
     rep_origin      4073..4910
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1/ARS416"
     CDS             complement(5245..6048)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(6049..6264)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(6289..6307)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6375..6479
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6480..6548,6549..7340)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6698..6717)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7511..8099
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8000..8019
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8253..8270
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
      241 atgcctgcag gtcgactcta gaggatctga attaattctt gaataataca taacttttct
      301 taaaagaatc aaagacagat aaaatttaag agatattaaa tattagtgag aagccgagaa
      361 ttttgtaaca ccaacataac actgacatct ttaacaactt ttaattatga tacatttctt
      421 acgtcatgat tgattattac agctatgctg acaaatgact cttgttgcat ggctacgaac
      481 cgggtaatac taagtgattg actcttgctg accttttatt aagaactaaa tggacaatat
      541 tatggagcat ttcatgtata aattggtgcg taaaatcgtt ggatctctct tctaagtaca
      601 tcctactata acaatcaaga aaaacaagaa aatcggacct cgagatcaag tatggattct
      661 agaacagttg gtatattagg agggggacaa ttgggacgta tgattgttga ggcagcaaac
      721 aggctcaaca ttaagacggt aatactagat gctgaaaatt ctcctgccaa acaaataagc
      781 aactccaatg accacgttaa tggctccttt tccaatcctc ttgatatcga aaaactagct
      841 gaaaaatgtg atgtgctaac gattgagatt gagcatgttg atgttcctac actaaagaat
      901 cttcaagtaa aacatcccaa attaaaaatt tacccttctc cagaaacaat cagattgata
      961 caagacaaat atattcaaaa agagcattta atcaaaaatg gtatagcagt tacccaaagt
     1021 gttcctgtgg aacaagccag tgagacgtcc ctattgaatg ttggaagaga tttgggtttt
     1081 ccattcgtct tgaagtcgag gactttggca tacgatggaa gaggtaactt cgttgtaaag
     1141 aataaggaaa tgattccgga agctttggaa gtactgaagg atcgtccttt gtacgccgaa
     1201 aaatgggcac catttactaa agaattagca gtcatgattg tgaggtctgt taggccagtc
     1261 acaatggcta gtgtcattgc acggctaccc aaaatattat accatcttct ctcaaatgaa
     1321 atcttttatg aaacaatccc cacagtggag gggtttcact ttgacgtttc caagactaag
     1381 caaagcattt aattgataca agttgctggg atcatttgta cccaaaatcc ggcgcggcgc
     1441 gggagaatgc agaggtcgca cggcggaggc ggacgcaaga gatccggtga atgaaacgaa
     1501 tcggcctcaa cgggggtttc actctgttac cgaggacttg gaaacgacgc tgacgagttt
     1561 caccaggatg aaactctttc cttctctctc atccccattt catgcaaata atcatttttt
     1621 attcagtctt acccctatta aatgtgcatg acacaccagt gaaaccccca ttgtgactgg
     1681 ccttaacggt ttagtgtttt cttacccaat tgtagagact atccacaagg acaatatttg
     1741 tgacttatgt tatgcgcctg ctagagttcc ggactccgtt caacttaagg cgaagttgtt
     1801 ggcagaaaat gcaatcaaat cttttcccgg ttgtggtata tttggtgtgg aaatgttcta
     1861 tttagaaaca ggggaattgc ttattaacga aattgcccca aggcctcaca actctggaca
     1921 ttataccatt gatgcttgcg tcacttctca atttgaagct catttgagat caatattgga
     1981 tttgccaatg ccaaagaatt tcacatcttt ctccaccatt acaacgaacg ccattatgct
     2041 aaatgttctt ggagacaaac atacaaaaga taaagagcta gaaacttgcg aaagagcatt
     2101 ggcgactcca ggttcctcag tgtacttata tggaaaagag tctagaccta acagaaaagt
     2161 aggtcacata aatattattg cctccagtat ggcggaatgt gaacaaaggc tgaactacat
     2221 tacaggtaga actgatattc caatcaaaat ctctgtcgct caaaagttgg acttggaagc
     2281 aatggtcaaa ccattggttg gaatcatcat gggatcagac tctgacttgc cggtaatgtc
     2341 tgccgcatgt gcggttttaa aagattttgg cgttccattt gaagtgacaa tagtctctgc
     2401 tcatagaact ccacatagga tgtcagcata tgctatttcc gcaagcaagc gtggaattaa
     2461 aacaattatc gctggagctg gtggggctgc tcacttgcca ggtatggtgg ctgcaatgac
     2521 accacttcct gtcatcggtg tgcccgtaaa aggttcttgt ctagatggag tagattcttt
     2581 acattcaatt gtgcaaatgc ctagaggtgt tccagtagct accgtcgcta ttaataatag
     2641 tacgaacgct gcgctgttgg ctgtcagact gcttggcgct tatgattcaa gttatacaac
     2701 gaaaatggaa cagtttttat taaagcaaga agaagaagtt cttgtcaaag cacaaaagtt
     2761 agaaactgtc ggttacgaag cttatctaga aaacaagtaa tatataagtt tattgatata
     2821 cttgtacagc aaataattat aaaatgatat acctattttt taggctttgt tatgattaca
     2881 tcaaatgtgg acttcataca tagaaatcaa cgcttacagg tgtccttttt taagaatttc
     2941 atacataaga tccccgggta ccgagctcga attcactggc cgtcgtttta caacgtcgtg
     3001 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     3061 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     3121 atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     3181 gcataatcgc tgggccattc tcatgaagaa tatcttgaat ttattgtcat attactagtt
     3241 ggtgtggaag tcctaatatc ggtgatcaat atagtggttg acatgctggc tagtcaacat
     3301 tgagcctttt gatcatgcaa atatattacg gtattttaca atcaaatatc aaacttaact
     3361 attgacttta taacttattt aggtggtaac attcttataa aaaagaaaaa aattactgca
     3421 aaacagtact agcttttaac ttgtatccta ggttatctat gctgtctcac catagagaat
     3481 attacctatt tcagaatgta tgtccatgat tcgccgggta aatacatata atacacaaat
     3541 ctggcttaat aaagtctata atatatctca taaagaagtg ctaaattggc tagtgctata
     3601 tatttttaag aaaatttctt ttgactaagt ccatatcgac tttgtaaaag ttcacattag
     3661 catacatata ttacacgagc cagaaatagt aacttttgcc taaatcacaa attgcaaaat
     3721 ttaattgctt gcaaaaggtc acatgcttat aatcaacttt tttaaaaatt taaaatactt
     3781 ttttattttt tatttttaaa cataaatgaa ataatttatt tattgtttat gattaccgaa
     3841 acataaaacc tgctcaagaa aaagaaactg ttttgtcctt ggaaaaaaag cactacctag
     3901 gagcggccaa aatgccgagg ctttcatagc ttaaactctt tacagaaaat aggcattata
     3961 gatcagttcg agttttctta ttcttccttc cggttttatc gtcacagttt tacagtaaat
     4021 aagtatcacc tcttagagtt cgatgataag ctgtcaaaca tgagaattaa ttccacatgt
     4081 taaaatagtg aaggagcatg ttcggcacac agtggaccga acgtggggta agtgcactag
     4141 ggtccggtta aacggatctc gcattgatga ggcaacgcta attatcaaca tatagattgt
     4201 tatctatctg catgaacacg aaatctttac ttgacgactt gaggctgatg gtgtttatgc
     4261 aaagaaacca ctgtgtttaa tatgtgtcac tgtttgatat tactgtcagc gtagaagata
     4321 atagtaaaag cggttaataa gtgtatttga gataagtgtg ataaagtttt tacagcgaaa
     4381 agacgataaa tacaagaaaa tgattacgag gatacggaga gaggtatgta catgtgtatt
     4441 tatatactaa gctgccggcg gttgtttgca agaccgagaa aaggctagca agaatcgggt
     4501 cattgtagcg tatgcgcctg tgaacattct cttcaacaag tttgattcca ttgcggtgaa
     4561 atggtaaaag tcaaccccct gcgatgtata ttttcctgta caatcaatca aaaagccaaa
     4621 tgatttagca ttatctttac atcttgttat tttacagatt ttatgtttag atcttttatg
     4681 cttgcttttc aaaaggcttg caggcaagtg cacaaacaat acttaaataa atactactca
     4741 gtaataacct atttcttagc atttttgacg aaatttgcta ttttgttaga gtcttttaca
     4801 ccatttgtct ccacacctcc gcttacatca acaccaataa cgccatttaa tctaagcgca
     4861 tcaccaacat tttctggcgt cagtccacca gctaacataa aatgtaagct ctcggggctc
     4921 tcttgccttc caacccagtc agaaatcgag ttccaatcca aaagttcacc tgtcccacct
     4981 gcttctgaat caaacaaggg aataaacgaa tgaggtttct gtgaagctgc actgagtagt
     5041 atgttgcagt cttttggaaa tacgagtctt ttaataactg gcaaaccgag gaactcttgg
     5101 tattcttgcc acgactcatc tccatgcagt tggacgatcg atgataagct gtcaaacatg
     5161 agaattgggt aataactgat ataattaaat tgaagctcta atttgtgagt ttagtataca
     5221 tgcatttact tataatacag ttttttagtt ttgctggccg catcttctca aatatgcttc
     5281 ccagcctgct tttctgtaac gttcaccctc taccttagca tcccttccct ttgcaaatag
     5341 tcctcttcca acaataataa tgtcagatcc tgtagagacc acatcatcca cggttctata
     5401 ctgttgaccc aatgcgtctc ccttgtcatc taaacccaca ccgggtgtca taatcaacca
     5461 atcgtaacct tcatctcttc cacccatgtc tctttgagca ataaagccga taacaaaatc
     5521 tttgtcgctc ttcgcaatgt caacagtacc cttagtatat tctccagtag atagggagcc
     5581 cttgcatgac aattctgcta acatcaaaag gcctctaggt tcctttgtta cttcttctgc
     5641 cgcctgcttc aaaccgctaa caatacctgg gcccaccaca ccgtgtgcat tcgtaatgtc
     5701 tgcccattct gctattctgt atacacccgc agagtactgc aatttgactg tattaccaat
     5761 gtcagcaaat tttctgtctt cgaagagtaa aaaattgtac ttggcggata atgcctttag
     5821 cggcttaact gtgccctcca tggaaaaatc agtcaagata tccacatgtg tttttagtaa
     5881 acaaattttg ggacctaatg cttcaactaa ctccagtaat tccttggtgg tacgaacatc
     5941 caatgaagca cacaagtttg tttgcttttc gtgcatgata ttaaatagct tggcagcaac
     6001 aggactagga tgagtagcag cacgttcctt atatgtagct ttcgacatga tttatcttcg
     6061 tttcctgcat gtttttgttc tgtgcagttg ggttaagaat actgggcaat ttcatgtttc
     6121 ttcaacacta catatgcgta tatataccaa tctaagtctg tgctccttcc ttcgttcttc
     6181 cttctgttcg gagattaccg aatcaaaaaa atttcaagga aaccgaaatc aaaaaaaaga
     6241 ataaaaaaaa aatgatgaat tgaaaagcta attcttgaag acgaaagggc ctcgtgatac
     6301 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     6361 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     6421 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     6481 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     6541 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     6601 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     6661 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     6721 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     6781 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     6841 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     6901 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     6961 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     7021 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     7081 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     7141 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     7201 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     7261 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     7321 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     7381 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     7441 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     7501 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     7561 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     7621 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     7681 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     7741 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     7801 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     7861 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     7921 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     7981 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     8041 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     8101 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     8161 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     8221 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     8281 a
//
