LOCUS       sequence-297343-        4272 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for SARS-CoV-2 nsp11.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4272)
  TITLE     SARS-CoV-2 protein Mammalian expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 4272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4133177
FEATURES             Location/Qualifiers
     source          1..4272
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             41..61
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             62..88
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    141..262
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(152..171)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     206..225
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      377..423
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      453..589
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    634..705
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     rep_origin      complement(931..1519)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1011..1030)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(2265..2333,2334..3125)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2483..2502)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      3138..3274
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    3426..3497
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(3502..3548)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    3566..3614
                     /note="synthetic polyadenylation signal"
     enhancer        3695..3998
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4000..4202
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4153..4173
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      join(4271..4272,1..8)
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 caccatggct gatgcacaat cgtttttaaa cgggtttgcg gaaaaccttt actttcagtc
       61 gtacccctat gacgtaccag attatgcgta agctagcttg actgactgag atacagcgta
      121 ccttcagctc acagacatga taagatacat tgatgagttt ggacaaacca caactagaat
      181 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
      241 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
      301 gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtattggccc
      361 atctctatcg gtatcgtagc ataacccctt ggggcctcta aacgggtctt gaggggtttt
      421 ttgtgcccct cgggccggat tgctatctac cggcattggc gcagaaaaaa atgcctgatg
      481 cgacgctgcg cgtcttatac tcccacatat gccagattca gcaacggata cggcttcccc
      541 aacttgccca cttccatacg tgtcctcctt accagaaatt tatccttaag gtcgtcagct
      601 atcctgcagg cgatctctcg atttcgatca agacattcct ttaatggtct tttctggaca
      661 ccactagggg tcagaagtag ttcatcaaac tttcttccct ccctaatctc attggttacc
      721 ttgggctatc gaaacttaat taaccagtca agtcagctac ttggcgagat cgacttgtct
      781 gggtttcgac tacgctcaga attgcgtcag tcaagttcga tctggtcctt gctattgcac
      841 ccgttctccg attacgagtt tcatttaaat catgtgagca aaaggccagc aaaaggccag
      901 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
      961 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     1021 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     1081 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     1141 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     1201 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     1261 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     1321 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     1381 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     1441 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     1501 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     1561 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     1621 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     1681 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     1741 ttcatccata gttgcattta aatttccgaa ctctccaagg ccctcgtcgg aaaatcttca
     1801 aacctttcgt ccgatccatc ttgcaggcta cctctcgaac gaactatcgc aagtctcttg
     1861 gccggccttg cgccttggct attgcttggc agcgcctatc gccaggtatt actccaatcc
     1921 cgaatatccg agatcgggat cacccgagag aagttcaacc tacatcctca atcccgatct
     1981 atccgagatc cgaggaatat cgaaatcggg gcgcgcctgg tgtaccgaga acgatcctct
     2041 cagtgcgagt ctcgacgatc catatcgttg cttggcagtc agccagtcgg aatccagctt
     2101 gggacccagg aagtccaatc gtcagatatt gtactcaagc ctggtcacgg cagcgtaccg
     2161 atctgtttaa acctagatat tgatagtctg atcggtcaac gtataatcga gtcctagctt
     2221 ttgcaaacat ctatcaagag acaggatcag caggaggctt tcgcatgagt attcaacatt
     2281 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     2341 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcgcgagtg ggttacatcg
     2401 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgctttccaa
     2461 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     2521 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tattcaccag
     2581 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     2641 ccatgagtga taacactgcg gccaacttac ttctgacaac gattggagga ccgaaggagc
     2701 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     2761 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     2821 caaccttgcg taaactatta actggcgaac tacttactct agcttcccgg caacagttga
     2881 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     2941 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     3001 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     3061 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     3121 ggtaaccgat tctaggtgca ttggcgcaga aaaaaatgcc tgatgcgacg ctgcgcgtct
     3181 tatactccca catatgccag attcagcaac ggatacggct tccccaactt gcccacttcc
     3241 atacgtgtcc tccttaccag aaatttatcc ttaagatccc gaatcgttta aactcgactc
     3301 tggctctatc gaatctccgt cgtttcgagc ttacgcgaac agccgtggcg ctcatttgct
     3361 cgtcgggcat cgaatctcgt cagctatcgt cagcttacct ttttggcagc gatcgcggct
     3421 cccgacatct tggaccatta gctccacagg tatcttcttc cctctagtgg tcataacagc
     3481 agcttcagct acctctcaat tcaaaaaacc cctcaagacc cgtttagagg ccccaagggg
     3541 ttatgctatc aatcgttgcg ttacacacac aaaaaaccaa cacacatcca tcttcgatgg
     3601 atagcgattt tattatctaa ctgctgatcg agtgtagcca gatctagtaa tcaattacgg
     3661 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     3721 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     3781 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     3841 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     3901 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     3961 ggcagtacat ctacgtatta gtcatcgcta ttaccatgct gatgcggttt tggcagtaca
     4021 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     4081 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     4141 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
     4201 ctggtttagt gaaccgtcag atcagatctt tgtcgatcct accatccact cgacacaccc
     4261 gccagcggcc gc
//
