LOCUS       sequence-297553-        4302 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for SARS-CoV-2 ORF10 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4302)
  TITLE     SARS-CoV-2 protein Mammalian expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 4302)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4136046
FEATURES             Location/Qualifiers
     source          1..4302
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    171..292
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(182..201)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     236..255
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      407..453
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      483..619
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    664..735
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     rep_origin      complement(961..1549)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1041..1060)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(2295..2363,2364..3155)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2513..2532)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      3168..3304
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    3456..3527
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(3532..3578)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    3596..3644
                     /note="synthetic polyadenylation signal"
     enhancer        3725..4028
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4030..4232
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4183..4203
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      join(4301..4302,1..8)
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 caccatgggg tacataaatg ttttcgcctt tccattcacg atctattcac tccttttatg
       61 taggatgaac tctcgcaatt acattgctca ggtagacgtc gtgaacttta acctaactta
      121 agctagcttg actgactgag atacagcgta ccttcagctc acagacatga taagatacat
      181 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
      241 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
      301 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa
      361 gtaaaacctc tacaaatgtg gtattggccc atctctatcg gtatcgtagc ataacccctt
      421 ggggcctcta aacgggtctt gaggggtttt ttgtgcccct cgggccggat tgctatctac
      481 cggcattggc gcagaaaaaa atgcctgatg cgacgctgcg cgtcttatac tcccacatat
      541 gccagattca gcaacggata cggcttcccc aacttgccca cttccatacg tgtcctcctt
      601 accagaaatt tatccttaag gtcgtcagct atcctgcagg cgatctctcg atttcgatca
      661 agacattcct ttaatggtct tttctggaca ccactagggg tcagaagtag ttcatcaaac
      721 tttcttccct ccctaatctc attggttacc ttgggctatc gaaacttaat taaccagtca
      781 agtcagctac ttggcgagat cgacttgtct gggtttcgac tacgctcaga attgcgtcag
      841 tcaagttcga tctggtcctt gctattgcac ccgttctccg attacgagtt tcatttaaat
      901 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
      961 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     1021 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     1081 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     1141 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     1201 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     1261 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     1321 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     1381 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     1441 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     1501 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     1561 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     1621 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     1681 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     1741 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcattta aatttccgaa
     1801 ctctccaagg ccctcgtcgg aaaatcttca aacctttcgt ccgatccatc ttgcaggcta
     1861 cctctcgaac gaactatcgc aagtctcttg gccggccttg cgccttggct attgcttggc
     1921 agcgcctatc gccaggtatt actccaatcc cgaatatccg agatcgggat cacccgagag
     1981 aagttcaacc tacatcctca atcccgatct atccgagatc cgaggaatat cgaaatcggg
     2041 gcgcgcctgg tgtaccgaga acgatcctct cagtgcgagt ctcgacgatc catatcgttg
     2101 cttggcagtc agccagtcgg aatccagctt gggacccagg aagtccaatc gtcagatatt
     2161 gtactcaagc ctggtcacgg cagcgtaccg atctgtttaa acctagatat tgatagtctg
     2221 atcggtcaac gtataatcga gtcctagctt ttgcaaacat ctatcaagag acaggatcag
     2281 caggaggctt tcgcatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     2341 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     2401 atcagttggg tgcgcgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     2461 agagttttcg ccccgaagaa cgctttccaa tgatgagcac ttttaaagtt ctgctatgtg
     2521 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     2581 ctcagaatga cttggttgag tattcaccag tcacagaaaa gcatcttacg gatggcatga
     2641 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     2701 ttctgacaac gattggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     2761 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     2821 gtgacaccac gatgcctgta gcaatggcaa caaccttgcg taaactatta actggcgaac
     2881 tacttactct agcttcccgg caacagttga tagactggat ggaggcggat aaagttgcag
     2941 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     3001 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     3061 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     3121 ctgagatagg tgcctcactg attaagcatt ggtaaccgat tctaggtgca ttggcgcaga
     3181 aaaaaatgcc tgatgcgacg ctgcgcgtct tatactccca catatgccag attcagcaac
     3241 ggatacggct tccccaactt gcccacttcc atacgtgtcc tccttaccag aaatttatcc
     3301 ttaagatccc gaatcgttta aactcgactc tggctctatc gaatctccgt cgtttcgagc
     3361 ttacgcgaac agccgtggcg ctcatttgct cgtcgggcat cgaatctcgt cagctatcgt
     3421 cagcttacct ttttggcagc gatcgcggct cccgacatct tggaccatta gctccacagg
     3481 tatcttcttc cctctagtgg tcataacagc agcttcagct acctctcaat tcaaaaaacc
     3541 cctcaagacc cgtttagagg ccccaagggg ttatgctatc aatcgttgcg ttacacacac
     3601 aaaaaaccaa cacacatcca tcttcgatgg atagcgattt tattatctaa ctgctgatcg
     3661 agtgtagcca gatctagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
     3721 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     3781 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     3841 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     3901 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     3961 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     4021 ttaccatgct gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
     4081 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
     4141 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
     4201 gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcag atcagatctt
     4261 tgtcgatcct accatccact cgacacaccc gccagcggcc gc
//
