LOCUS       sequence-297559-        8586 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast expression plasmid for SARS-CoV-2 S (surface glycoprotein).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8586)
  TITLE     SARS-CoV-2 protein Yeast expression constructs, tagged, for protein 
            expression and/or purification
REFERENCE   2  (bases 1 to 8586)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4046680
FEATURES             Location/Qualifiers
     source          1..8586
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        25..466
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    25..142
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     437..460
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     complement(4341..4359)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             4710..5513
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      5596..6578
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     terminator      6656..6685
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      6707..7294
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(7306..7333)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(7357..7548)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(7549..8340,8341..8409))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8172..8191
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8410..8512)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(8513..8544)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 gcggccgctt gctatattga agtacggatt agaagccgcc gagcgggcga cagccctccg
       61 acggaagact ctcctccgtg cgtcctcgtc ttcaccggtc gcgttcctga aacgcagatg
      121 tgcctcgcgc cgcactgctc cgaacaataa agattctaca atactagctt ttatggttat
      181 gaagaggaaa aattggcagt aacctggccc cacaaacctt caaattaacg aatcaaatta
      241 acaaccatag gatgataatg cgattagttt tttagcctta tttctggggt aattaatcag
      301 cgaagcgatg atttttgatc tattaacaga tatataaatg gaaaagctgc ataaccactt
      361 taactaatac tttcaacatt ttcagtttgt attacttctt attcaaatgt cataaaagta
      421 tcaacaaaaa attgttaata tacctctata ctttaacgtc aaggagaaaa aactataatg
      481 ttcgtctttt tagttttgct tccattggtg agctcccaat gcgttaatct gacaactcgc
      541 acgcagctac ctccagcata cactaactct ttcaccagag gcgtctatta ccccgacaaa
      601 gtatttcgtt cttcggtttt acatagtacc caagatttgt tcttgccatt cttttcaaac
      661 gttacttggt ttcacgccat acacgtttct ggtaccaatg gtactaagag gttcgataac
      721 ccagtgttgc cattcaatga cggtgtctac ttcgcttcca cagaaaagtc taacatcatt
      781 agaggttgga tctttggaac cacgcttgat tccaagactc aatctttgtt aattgtcaac
      841 aacgctacta atgttgtaat taaagtctgt gagttccaat tctgtaacga cccgtttttg
      901 ggtgtttatt accataagaa taataaaagc tggatggaat ccgaatttcg ggtttattca
      961 agtgctaaca actgtacctt cgaatacgtc agtcaacctt tcctgatgga tttggagggt
     1021 aagcaaggca acttcaagaa cttgagagaa tttgtcttca aaaatatcga cggttacttt
     1081 aagatctact ctaagcacac accaattaac ctagttagag atttacctca gggtttctct
     1141 gctttggaac cactcgttga tttgccgatt ggtatcaaca ttacgagatt tcaaactttg
     1201 ctagccttac accgatcata tttaactcct ggtgactcct cgtctggatg gactgcgggt
     1261 gctgctgcct actatgtcgg ttatttgcag ccaagaacct ttctgctcaa gtacaatgaa
     1321 aacgggacaa ttaccgatgc tgtagactgt gctttggatc cattgagcga aactaagtgc
     1381 accctaaaat ctttcacagt tgaaaagggt atataccaaa ctagcaactt tagagttcaa
     1441 ccaactgaat caatagtcag attcccaaat atcaccaact tgtgtccatt cggtgaggtt
     1501 tttaatgcca caaggtttgc ttctgtgtac gcttggaacc gtaagagaat ctccaattgt
     1561 gtggcagatt actctgtact atataacagt gcttccttct ctactttcaa atgttacggg
     1621 gtttcgccaa ccaagcttaa cgacttatgc ttcactaatg tttacgcaga ctcctttgtc
     1681 attagaggcg atgaagtaag acaaatcgcg ccaggtcaaa ccggtaagat tgctgattac
     1741 aactacaaat tgccagacga cttcaccggt tgcgtgatcg cttggaatag caacaacttg
     1801 gattcgaagg ttggtgggaa ctataactat ttgtaccgcc tatttcgtaa gtctaatttg
     1861 aaacctttcg aaagagacat aagtaccgag atttatcaag ctggttctac tccttgtaat
     1921 ggggtcgaag gttttaactg ttacttcccc ttacaatctt acggttttca gccaaccaac
     1981 ggagttggtt accaaccata tagagttgtt gtcctgtctt ttgaactatt gcatgcccca
     2041 gctacagtct gcggtcccaa aaagtccacg aacttggtta aaaataagtg tgtcaacttc
     2101 aatttcaatg gtcttactgg tactggcgta ttgaccgaat ccaacaagaa gttcctgcca
     2161 ttccaacaat tcggtagaga tattgcagat acgaccgatg cggtccggga tcctcaaact
     2221 ttggaaattt tggacatcac tccatgttcc tttggtggtg ttagcgttat tacccctgga
     2281 accaacactt ctaatcaggt cgccgtttta taccaagacg tcaactgtac ggaagtgcca
     2341 gttgctatcc acgctgacca actaacccct acttggagag tctactctac tggttctaat
     2401 gttttccaaa caagagctgg ttgtttgata ggtgctgagc atgtcaacaa ctcctatgaa
     2461 tgtgacattc caattggtgc cggaatctgt gcatcctacc aaacacagac taattcacca
     2521 agaagagcta gaagtgttgc ttcacaaagt atcattgcat acaccatgag cctaggtgcc
     2581 gaaaactcag tcgcatacag taacaattct attgctatac caactaattt cactatttct
     2641 gttactactg aaatcttacc tgtctctatg acaaagacct cagtggattg tacaatgtat
     2701 atctgtggtg actctactga gtgttctaat ttgttgttac aatacggttc cttctgcaca
     2761 caattgaacc gtgctcttac gggtattgct gtcgaacaag acaaaaacac ccaggaagta
     2821 tttgcccaag tcaagcaaat ctacaagacc ccacccatta aggatttcgg cggtttcaac
     2881 tttagccaaa tcttgccaga tccatcaaag ccatccaaga gatcgtttat cgaagattta
     2941 ttatttaata aagttacgtt agccgacgct ggattcatca agcaatatgg cgattgcttg
     3001 ggtgacattg cagcccgaga cttgatatgt gcgcagaagt tcaatggtct taccgtgttg
     3061 cccccactat tgactgatga aatgatcgct caatatacct cagcgctctt ggctgggacg
     3121 attacttctg gttggacatt tggtgctggt gctgcattgc aaatcccatt tgctatgcaa
     3181 atggcttaca gattcaacgg gataggagtt actcaaaacg ttctgtatga aaaccaaaag
     3241 ctaatcgcta atcaattcaa ctcggccatt ggtaagattc aagactcttt gtcctcgact
     3301 gcttcggcgc ttggtaaact acaagacgtt gttaaccaga atgctcaagc tttgaacacc
     3361 ctcgtaaaac agttgtcctc taacttcggg gccatttctt ccgtactcaa cgatatctta
     3421 tctaggttgg ataaggttga ggccgaagta caaatcgata gattgatcac cggaagattg
     3481 caatcgctac aaacctacgt tactcaacaa cttatccgcg ctgctgaaat tagagctagt
     3541 gctaatttag ccgcgaccaa gatgagtgaa tgtgtcctag gtcagtctaa gcgtgttgac
     3601 ttctgtggta aaggttatca tttgatgtcc tttccccaat ctgccccgca cggtgtcgtc
     3661 tttttgcatg ttacttacgt gccggctcag gaaaagaact tcactacagc tcctgccatc
     3721 tgtcacgatg gtaaagctca cttcccaaga gaaggtgttt tcgtttctaa tggtactcac
     3781 tggttcgtca cacaaagaaa cttctacgag ccccaaatta ttactactga caacaccttc
     3841 gtatccggta attgtgacgt tgtcatcggt attgtcaaca acaccgttta tgatccactc
     3901 caaccagaat tagattcctt caaggaagaa ttggataaat actttaagaa tcacacctcc
     3961 cctgacgttg atctaggtga catatctggc attaacgctt ccgtggttaa cattcaaaag
     4021 gaaatagaca ggttaaatga ggttgccaag aacttgaacg agtcattgat tgatctccaa
     4081 gaactaggta agtacgaaca atacattaaa tggccgtggt acatctggtt gggttttatc
     4141 gcagggctga ttgctattgt tatggtgacc atcatgttgt gctgtatgac ctcatgttgt
     4201 tcttgtttaa agggttgttg cagctgcggt tcttgttgca agttcgatga agacgactct
     4261 gaacctgtat tgaagggtgt taaattgcac tacacgtaaa caggcccctt ttcctttgtc
     4321 gatatcatgt aattagttat gtcacgctta cattcacgcc ctcctcccac atccgctcta
     4381 accgaaaagg aaggagttag acaacctgaa gtctaggtcc ctatttattt tttttaatag
     4441 ttatgttagt attaagaacg ttatttatat ttcaaatttt tctttttttt ctgtacaaag
     4501 cggccgccga accactagtg cactgcagta cacggtttcc ttgaaatttt tttgattcgg
     4561 taatctccga acagaaggaa gaacgaagga aggagcacag acttagattg gtatatatac
     4621 gcatatgtag tgttgaagaa acatgaaatt gcccagtatt cttaacccaa ctgcacagaa
     4681 caaaaacgtg caggaaacga agataaatca tgtcgaaagc tacatataag gaacgtgctg
     4741 ctactcatcc tagtcctgtt gctgccaagc tatttaatat catgcacgaa aagcaaacaa
     4801 acttgtgtgc ttcattggat gttcgtacca ccaaggaatt actggagtta gttgaagcat
     4861 taggtcccaa aatttgttta ctaaaaacac atgtggatat cttgactgat ttttccatgg
     4921 agggcacagt taagccgcta aaggcattat ccgccaagta caatttttta ctcttcgagg
     4981 acagaaaatt tgctgacatt ggtaatacag tcaaattgca gtactctgcg ggtgtataca
     5041 gaatagcaga atgggcagac attacgaatg cacacggtgt ggtgggccca ggtattgtta
     5101 gcggtttgaa gcaggcggca gaagaagtaa caaaggaacc tagaggcctt ttgatgttag
     5161 cagaattgtc atgcaagggc tccctatcta ctggagaata tactaagggt actgttgaca
     5221 ttgcgaagag cgacaaagat tttgttatcg gctttattgc tcaaagagac atgggtggaa
     5281 gagatgaagg ttacgattgg ttgattatga cacccggtgt gggtttagat gacaagggag
     5341 atgcattggg tcaacagtat agaaccgtgg atgatgtggt ttctacagga tctgacatta
     5401 ttattgttgg aagaggacta tttgcaaagg gaagggatgc taaggtagag ggtgaacgtt
     5461 acagaaaagc aggctgggaa gcatatttga gaagatgcgg ccagcaaaac taaaaaactg
     5521 tattataagt aaatgcatgt atactaaact cacaaattag agcttcaatt taattatatc
     5581 agttattacc cgagtaacga agcatctgtg cttcattttg tagaacaaaa atgcaacgcg
     5641 agagcgctaa tttttcaaac aaagaatctg agctgcattt ttacagaaca gaaatgcaac
     5701 gcgaaagcgc tattttacca acgaagaatc tgtgcttcat ttttgtaaaa caaaaatgca
     5761 acgcgagagc gctaattttt caaacaaaga atctgagctg catttttaca gaacagaaat
     5821 gcaacgcgag agcgctattt taccaacaaa gaatctatac ttcttttttg ttctacaaaa
     5881 atgcatcccg agagcgctat ttttctaaca aagcatctta gattactttt tttctccttt
     5941 gtgcgctcta taatgcagtc tcttgataac tttttgcact gtaggtccgt taaggttaga
     6001 agaaggctac tttggtgtct attttctctt ccataaaaaa agcctgactc cacttcccgc
     6061 gtttactgat tactagcgaa gctgcgggtg cattttttca agataaaggc atccccgatt
     6121 atattctata ccgatgtgga ttgcgcatac tttgtgaaca gaaagtgata gcgttgatga
     6181 ttcttcattg gtcagaaaat tatgaacggt ttcttctatt ttgtctctat atactacgta
     6241 taggaaatgt ttacattttc gtattgtttt cgattcactc tatgaatagt tcttactaca
     6301 atttttttgt ctaaagagta atactagaga taaacataaa aaatgtagag gtcgagttta
     6361 gatgcaagtt caaggagcga aaggtggatg ggtaggttat atagggatat agcacagaga
     6421 tatatagcaa agagatactt ttgagcaatg tttgtggaag cggtattcgc aatattttag
     6481 tagctcgtta cagtccggtg cgtttttggt tttttgaaag tgcgtcatca gagcgctttt
     6541 ggttttcaaa agcgctctga agttcctata ctttctagct agagaatagg aacttcccga
     6601 gcggccgcgt gttacaagct tgactccagc gtaactggac tgcaatcaac tcactggctc
     6661 accttcacgg gtgggccttt cttcggtaga aaatcaaagg atcttcttga gatccttttt
     6721 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     6781 tgccggatca agagctacca actctttttc cgaggtaact ggcttcagca gagcgcagat
     6841 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     6901 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     6961 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     7021 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     7081 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     7141 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc caggggtaaa
     7201 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc atcgattttt
     7261 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcag aaaggcccac
     7321 ccgaaggtga gccaggtgat tacatttggg ccctcatcag aggttttcac cgtcatcacc
     7381 gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat
     7441 gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct
     7501 tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactt accaatgctt
     7561 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     7621 ccccgtcgtg tagataacta cgatgcggga gggcttacca tctggcccca gtgctgcaat
     7681 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     7741 gagggccgag cgcagaagtg atcctgcaac tttatccgcc tccatccagt ctattaattg
     7801 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     7861 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     7921 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     7981 cggtcctccg atcgttgcca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     8041 agcactgcat aattctctta ctgtcatgcc atccgtgaga tgcttttctg tgactggtga
     8101 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     8161 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     8221 acgttcttcg gggcgtaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     8281 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     8341 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     8401 aatactcatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg
     8461 tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgccgat caagtcaaaa
     8521 gcctccggtc ggaggctttt gactttctgc tatggaggtc aggtatgatt taaatggtca
     8581 gttaga
//
