LOCUS       sequence-29759-e         529 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-FGFR1
FEATURES             Location/Qualifiers
     source          1..529
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    73..97
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(479..496)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga tgagcaatgc
       61 ttttttataa tgccaacttt gtacaaaaaa gttggcacca tgtggagctg gaagtgcctc
      121 ctcttctggg ctgtgctggt cacagccaca ctctgcaccg ctaggccgtc cccgaccttg
      181 cctgaacaag cccagccctg gggagcccct gtggaagtgg agtccttcct ggtccacccc
      241 ggtgacctgc tgcagcttcg ctgtcggctg cgggacgatg tgcagagcat caactggctg
      301 cgggacgggg tgcagctggc ggaaagcaac cgcacccgca tcacagggga ggaggtggag
      361 gtgcaggact ccgtgcccgc agactccggc ctctatgctt gcgtaaccag cagcccctcg
      421 ggcagtgaca ccacctactt ctccgtcaat gtttcagatg ctctcccctc ctcggaggat
      481 gatgatgatg atgatgactc ctcttcagag gagaaagaaa cagataaca
//
