LOCUS       sequence-297595-        8539 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast expression plasmid for SARS-CoV-2 S (surface glycoprotein).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8539)
  TITLE     SARS-CoV-2 protein Yeast expression constructs, tagged, for protein 
            expression and/or purification
REFERENCE   2  (bases 1 to 8539)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4046640
FEATURES             Location/Qualifiers
     source          1..8539
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..417
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     primer_bind     complement(4294..4312)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             4663..5466
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      5549..6531
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     terminator      6609..6638
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      6660..7247
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(7259..7286)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(7310..7501)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(7502..8293,8294..8362))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8125..8144
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8363..8465)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(8466..8497)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 gcggccgccc cacacaccat agcttcaaaa tgtttctact ccttttttac tcttccagat
       61 tttctcggac tccgcgcatc gccgtaccac ttcaaaacac ccaagcacag catactaaat
      121 ttcccctctt tcttcctcta gggtgtcgtt aattacccgt actaaaggtt tggaaaagaa
      181 aaaagagacc gcctcgtttc tttttcttcg tcgaaaaagg caataaaaat ttttatcacg
      241 tttctttttc ttgaaaattt ttttttttga tttttttctc tttcgatgac ctcccattga
      301 tatttaagtt aataaacggt cttcaatttc tcaagtttca gtttcatttt tcttgttcta
      361 ttacaacttt ttttacttct tgctcattag aaagaaagca tagcaatcta atctaagttt
      421 taattacaaa atgttcgtct ttttagtttt gcttccattg gtgagctccc aatgcgttaa
      481 tctgacaact cgcacgcagc tacctccagc atacactaac tctttcacca gaggcgtcta
      541 ttaccccgac aaagtatttc gttcttcggt tttacatagt acccaagatt tgttcttgcc
      601 attcttttca aacgttactt ggtttcacgc catacacgtt tctggtacca atggtactaa
      661 gaggttcgat aacccagtgt tgccattcaa tgacggtgtc tacttcgctt ccacagaaaa
      721 gtctaacatc attagaggtt ggatctttgg aaccacgctt gattccaaga ctcaatcttt
      781 gttaattgtc aacaacgcta ctaatgttgt aattaaagtc tgtgagttcc aattctgtaa
      841 cgacccgttt ttgggtgttt attaccataa gaataataaa agctggatgg aatccgaatt
      901 tcgggtttat tcaagtgcta acaactgtac cttcgaatac gtcagtcaac ctttcctgat
      961 ggatttggag ggtaagcaag gcaacttcaa gaacttgaga gaatttgtct tcaaaaatat
     1021 cgacggttac tttaagatct actctaagca cacaccaatt aacctagtta gagatttacc
     1081 tcagggtttc tctgctttgg aaccactcgt tgatttgccg attggtatca acattacgag
     1141 atttcaaact ttgctagcct tacaccgatc atatttaact cctggtgact cctcgtctgg
     1201 atggactgcg ggtgctgctg cctactatgt cggttatttg cagccaagaa cctttctgct
     1261 caagtacaat gaaaacggga caattaccga tgctgtagac tgtgctttgg atccattgag
     1321 cgaaactaag tgcaccctaa aatctttcac agttgaaaag ggtatatacc aaactagcaa
     1381 ctttagagtt caaccaactg aatcaatagt cagattccca aatatcacca acttgtgtcc
     1441 attcggtgag gtttttaatg ccacaaggtt tgcttctgtg tacgcttgga accgtaagag
     1501 aatctccaat tgtgtggcag attactctgt actatataac agtgcttcct tctctacttt
     1561 caaatgttac ggggtttcgc caaccaagct taacgactta tgcttcacta atgtttacgc
     1621 agactccttt gtcattagag gcgatgaagt aagacaaatc gcgccaggtc aaaccggtaa
     1681 gattgctgat tacaactaca aattgccaga cgacttcacc ggttgcgtga tcgcttggaa
     1741 tagcaacaac ttggattcga aggttggtgg gaactataac tatttgtacc gcctatttcg
     1801 taagtctaat ttgaaacctt tcgaaagaga cataagtacc gagatttatc aagctggttc
     1861 tactccttgt aatggggtcg aaggttttaa ctgttacttc cccttacaat cttacggttt
     1921 tcagccaacc aacggagttg gttaccaacc atatagagtt gttgtcctgt cttttgaact
     1981 attgcatgcc ccagctacag tctgcggtcc caaaaagtcc acgaacttgg ttaaaaataa
     2041 gtgtgtcaac ttcaatttca atggtcttac tggtactggc gtattgaccg aatccaacaa
     2101 gaagttcctg ccattccaac aattcggtag agatattgca gatacgaccg atgcggtccg
     2161 ggatcctcaa actttggaaa ttttggacat cactccatgt tcctttggtg gtgttagcgt
     2221 tattacccct ggaaccaaca cttctaatca ggtcgccgtt ttataccaag acgtcaactg
     2281 tacggaagtg ccagttgcta tccacgctga ccaactaacc cctacttgga gagtctactc
     2341 tactggttct aatgttttcc aaacaagagc tggttgtttg ataggtgctg agcatgtcaa
     2401 caactcctat gaatgtgaca ttccaattgg tgccggaatc tgtgcatcct accaaacaca
     2461 gactaattca ccaagaagag ctagaagtgt tgcttcacaa agtatcattg catacaccat
     2521 gagcctaggt gccgaaaact cagtcgcata cagtaacaat tctattgcta taccaactaa
     2581 tttcactatt tctgttacta ctgaaatctt acctgtctct atgacaaaga cctcagtgga
     2641 ttgtacaatg tatatctgtg gtgactctac tgagtgttct aatttgttgt tacaatacgg
     2701 ttccttctgc acacaattga accgtgctct tacgggtatt gctgtcgaac aagacaaaaa
     2761 cacccaggaa gtatttgccc aagtcaagca aatctacaag accccaccca ttaaggattt
     2821 cggcggtttc aactttagcc aaatcttgcc agatccatca aagccatcca agagatcgtt
     2881 tatcgaagat ttattattta ataaagttac gttagccgac gctggattca tcaagcaata
     2941 tggcgattgc ttgggtgaca ttgcagcccg agacttgata tgtgcgcaga agttcaatgg
     3001 tcttaccgtg ttgcccccac tattgactga tgaaatgatc gctcaatata cctcagcgct
     3061 cttggctggg acgattactt ctggttggac atttggtgct ggtgctgcat tgcaaatccc
     3121 atttgctatg caaatggctt acagattcaa cgggatagga gttactcaaa acgttctgta
     3181 tgaaaaccaa aagctaatcg ctaatcaatt caactcggcc attggtaaga ttcaagactc
     3241 tttgtcctcg actgcttcgg cgcttggtaa actacaagac gttgttaacc agaatgctca
     3301 agctttgaac accctcgtaa aacagttgtc ctctaacttc ggggccattt cttccgtact
     3361 caacgatatc ttatctaggt tggataaggt tgaggccgaa gtacaaatcg atagattgat
     3421 caccggaaga ttgcaatcgc tacaaaccta cgttactcaa caacttatcc gcgctgctga
     3481 aattagagct agtgctaatt tagccgcgac caagatgagt gaatgtgtcc taggtcagtc
     3541 taagcgtgtt gacttctgtg gtaaaggtta tcatttgatg tcctttcccc aatctgcccc
     3601 gcacggtgtc gtctttttgc atgttactta cgtgccggct caggaaaaga acttcactac
     3661 agctcctgcc atctgtcacg atggtaaagc tcacttccca agagaaggtg ttttcgtttc
     3721 taatggtact cactggttcg tcacacaaag aaacttctac gagccccaaa ttattactac
     3781 tgacaacacc ttcgtatccg gtaattgtga cgttgtcatc ggtattgtca acaacaccgt
     3841 ttatgatcca ctccaaccag aattagattc cttcaaggaa gaattggata aatactttaa
     3901 gaatcacacc tcccctgacg ttgatctagg tgacatatct ggcattaacg cttccgtggt
     3961 taacattcaa aaggaaatag acaggttaaa tgaggttgcc aagaacttga acgagtcatt
     4021 gattgatctc caagaactag gtaagtacga acaatacatt aaatggccgt ggtacatctg
     4081 gttgggtttt atcgcagggc tgattgctat tgttatggtg accatcatgt tgtgctgtat
     4141 gacctcatgt tgttcttgtt taaagggttg ttgcagctgc ggttcttgtt gcaagttcga
     4201 tgaagacgac tctgaacctg tattgaaggg tgttaaattg cactacacgt aaacaggccc
     4261 cttttccttt gtcgatatca tgtaattagt tatgtcacgc ttacattcac gccctcctcc
     4321 cacatccgct ctaaccgaaa aggaaggagt tagacaacct gaagtctagg tccctattta
     4381 ttttttttaa tagttatgtt agtattaaga acgttattta tatttcaaat ttttcttttt
     4441 tttctgtaca aagcggccgc cgaaccacta gtgcactgca gtacacggtt tccttgaaat
     4501 ttttttgatt cggtaatctc cgaacagaag gaagaacgaa ggaaggagca cagacttaga
     4561 ttggtatata tacgcatatg tagtgttgaa gaaacatgaa attgcccagt attcttaacc
     4621 caactgcaca gaacaaaaac gtgcaggaaa cgaagataaa tcatgtcgaa agctacatat
     4681 aaggaacgtg ctgctactca tcctagtcct gttgctgcca agctatttaa tatcatgcac
     4741 gaaaagcaaa caaacttgtg tgcttcattg gatgttcgta ccaccaagga attactggag
     4801 ttagttgaag cattaggtcc caaaatttgt ttactaaaaa cacatgtgga tatcttgact
     4861 gatttttcca tggagggcac agttaagccg ctaaaggcat tatccgccaa gtacaatttt
     4921 ttactcttcg aggacagaaa atttgctgac attggtaata cagtcaaatt gcagtactct
     4981 gcgggtgtat acagaatagc agaatgggca gacattacga atgcacacgg tgtggtgggc
     5041 ccaggtattg ttagcggttt gaagcaggcg gcagaagaag taacaaagga acctagaggc
     5101 cttttgatgt tagcagaatt gtcatgcaag ggctccctat ctactggaga atatactaag
     5161 ggtactgttg acattgcgaa gagcgacaaa gattttgtta tcggctttat tgctcaaaga
     5221 gacatgggtg gaagagatga aggttacgat tggttgatta tgacacccgg tgtgggttta
     5281 gatgacaagg gagatgcatt gggtcaacag tatagaaccg tggatgatgt ggtttctaca
     5341 ggatctgaca ttattattgt tggaagagga ctatttgcaa agggaaggga tgctaaggta
     5401 gagggtgaac gttacagaaa agcaggctgg gaagcatatt tgagaagatg cggccagcaa
     5461 aactaaaaaa ctgtattata agtaaatgca tgtatactaa actcacaaat tagagcttca
     5521 atttaattat atcagttatt acccgagtaa cgaagcatct gtgcttcatt ttgtagaaca
     5581 aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca tttttacaga
     5641 acagaaatgc aacgcgaaag cgctatttta ccaacgaaga atctgtgctt catttttgta
     5701 aaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa agaatctgag ctgcattttt
     5761 acagaacaga aatgcaacgc gagagcgcta ttttaccaac aaagaatcta tacttctttt
     5821 ttgttctaca aaaatgcatc ccgagagcgc tatttttcta acaaagcatc ttagattact
     5881 ttttttctcc tttgtgcgct ctataatgca gtctcttgat aactttttgc actgtaggtc
     5941 cgttaaggtt agaagaaggc tactttggtg tctattttct cttccataaa aaaagcctga
     6001 ctccacttcc cgcgtttact gattactagc gaagctgcgg gtgcattttt tcaagataaa
     6061 ggcatccccg attatattct ataccgatgt ggattgcgca tactttgtga acagaaagtg
     6121 atagcgttga tgattcttca ttggtcagaa aattatgaac ggtttcttct attttgtctc
     6181 tatatactac gtataggaaa tgtttacatt ttcgtattgt tttcgattca ctctatgaat
     6241 agttcttact acaatttttt tgtctaaaga gtaatactag agataaacat aaaaaatgta
     6301 gaggtcgagt ttagatgcaa gttcaaggag cgaaaggtgg atgggtaggt tatataggga
     6361 tatagcacag agatatatag caaagagata cttttgagca atgtttgtgg aagcggtatt
     6421 cgcaatattt tagtagctcg ttacagtccg gtgcgttttt ggttttttga aagtgcgtca
     6481 tcagagcgct tttggttttc aaaagcgctc tgaagttcct atactttcta gctagagaat
     6541 aggaacttcc cgagcggccg cgtgttacaa gcttgactcc agcgtaactg gactgcaatc
     6601 aactcactgg ctcaccttca cgggtgggcc tttcttcggt agaaaatcaa aggatcttct
     6661 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     6721 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaggta actggcttca
     6781 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     6841 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     6901 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     6961 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     7021 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     7081 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     7141 ttccaggggt aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     7201 agcatcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     7261 cagaaaggcc cacccgaagg tgagccaggt gattacattt gggccctcat cagaggtttt
     7321 caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa
     7381 gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg
     7441 ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca
     7501 cttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     7561 tagttgcctg actccccgtc gtgtagataa ctacgatgcg ggagggctta ccatctggcc
     7621 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     7681 accagccagc cgggagggcc gagcgcagaa gtgatcctgc aactttatcc gcctccatcc
     7741 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     7801 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     7861 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     7921 cggttagctc cttcggtcct ccgatcgttg ccagaagtaa gttggccgca gtgttatcac
     7981 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgtg agatgctttt
     8041 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     8101 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     8161 tcatcattgg aaaacgttct tcggggcgta aactctcaag gatcttaccg ctgttgagat
     8221 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     8281 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     8341 cacggaaatg ttgaatactc attttagctt ccttagctcc tgaaaatctc gataactcaa
     8401 aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc
     8461 gatcaagtca aaagcctccg gtcggaggct tttgactttc tgctatggag gtcaggtatg
     8521 atttaaatgg tcagttaga
//
