LOCUS       sequence-297611-        6723 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for SARS-CoV-2 ORF6 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6723)
  TITLE     SARS-CoV-2 protein Mammalian expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 6723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4252991
FEATURES             Location/Qualifiers
     source          1..6723
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    256..377
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(267..286)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     321..340
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      492..538
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      568..704
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    749..820
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     rep_origin      complement(1046..1634)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1126..1145)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2134..3343
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3180..3199
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             3347..4375
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     polyA_signal    4402..4450
                     /note="synthetic polyadenylation signal"
     CDS             join(4716..4784,4785..5576)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4934..4953)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      5589..5725
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    5877..5948
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(5953..5999)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    6017..6065
                     /note="synthetic polyadenylation signal"
     enhancer        6146..6449
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6451..6653
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6604..6624
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 caccatgttt catctcgttg actttcaggt tactatagca gagatattac taattattat
       61 gaggactttt aaagtttcca tttggaatct tgattacatc ataaacctca taattaaaaa
      121 tttatctaag tcactaactg agaataaata ttctcaatta gatgaagagc aaccaatgga
      181 gattgattaa ggatccgcag gcctctgcta gcttgactga ctgagataca gcgtaccttc
      241 agctcacaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
      301 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
      361 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
      421 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatt ggcccatctc
      481 tatcggtatc gtagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgtg
      541 cccctcgggc cggattgcta tctaccggca ttggcgcaga aaaaaatgcc tgatgcgacg
      601 ctgcgcgtct tatactccca catatgccag attcagcaac ggatacggct tccccaactt
      661 gcccacttcc atacgtgtcc tccttaccag aaatttatcc ttaaggtcgt cagctatcct
      721 gcaggcgatc tctcgatttc gatcaagaca ttcctttaat ggtcttttct ggacaccact
      781 aggggtcaga agtagttcat caaactttct tccctcccta atctcattgg ttaccttggg
      841 ctatcgaaac ttaattaacc agtcaagtca gctacttggc gagatcgact tgtctgggtt
      901 tcgactacgc tcagaattgc gtcagtcaag ttcgatctgg tccttgctat tgcacccgtt
      961 ctccgattac gagtttcatt taaatcatgt gagcaaaagg ccagcaaaag gccaggaacc
     1021 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     1081 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     1141 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     1201 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     1261 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     1321 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     1381 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     1441 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     1501 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     1561 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     1621 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     1681 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     1741 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     1801 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     1861 ccatagttgc atttaaattt ccgaactctc caaggccctc gtcggaaaat cttcaaacct
     1921 ttcgtccgat ccatcttgca ggctacctct cgaacgaact atcgcaagtc tcttggccgg
     1981 ccttgcgcct tggctattgc ttggcagcgc ctatcgccag gtattactcc aatcccgaat
     2041 atccgagatc gggatcaccc gagagaagtt caacctacat cctcaatccc gatctatccg
     2101 agatccgagg aatatcgaaa tcggggcgcg cctggcctcc gcgccgggtt ttggcgcctc
     2161 ccgcgggcgc ccccctcgtc acggcgagcg ctgccacgtc agacgaaggg cgcaggagcg
     2221 tcctgatcct tccgcccgga cgctcaggac agcggcccgc tgctcataag actcggcctt
     2281 agaaccccag tatcagcaga aggacatttt aggacgggac ttgggtgact ctagggcact
     2341 ggttttcttt ccagagagcg gaacaggcga ggaaaagtag tcccttctcg gcgattctgc
     2401 ggagggatct ccgtggggcg gtgaacgccg atgattatat aaggacgcgc cgggtgtggc
     2461 acagctagtt ccgtcgcagc cgggatttgg gtcgcggttc ttgtttgtgg atcgctgtga
     2521 tcgtcacttg gtgagtagcg ggctgctggg ctggccgggg ctttcgtggc cgccgggccg
     2581 ctcggtggga cggaagcgtg tggagagacc gccaagggct gtagtctggg tccgcgagca
     2641 aggttgccct gaactggggg ttggggggag cgcagcaaaa tggcggctgt tcccgagtct
     2701 tgaatggaag acgcttgtga ggcgggctgt gaggtcgttg aaacaaggtg gggggcatgg
     2761 tgggcggcaa gaacccaagg tcttgagccc ttcgctaatg cgggaaagct cttattcggg
     2821 tgagatgggc tgggcaccat ctggggaccc tgacgtgaag tttgtcactg actggagaac
     2881 tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt gccgttgggc agtgcacccg
     2941 tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt tggcttataa
     3001 tgcagggtgg ggccaccagc cggtaggtgt gcggtaggct tttctccgtc gcaggacgca
     3061 gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg ccggacctct ggtgagggga
     3121 gggataagtg aggcgtcagt ttctttggtc ggttttatgt acctatcttc ttaagtagct
     3181 gaagctccgg ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg aagtttttta
     3241 ggcacctttt gaaatgtaat catttgggtc aatatgtaat tttcagtgtt agacttgtaa
     3301 attgtccgct aaattctggc cgtttttggc ttttttgtta gacaacatgg gtaaaaagcc
     3361 tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga
     3421 cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg
     3481 tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta
     3541 tcggcacttt gcatccgccg cgctcccgat tccggaagtg cttgacattg gggagttcag
     3601 cgagagcctg acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc
     3661 tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag gcaatggatg ccatcgctgc
     3721 cgccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata
     3781 cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac
     3841 tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg
     3901 ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt
     3961 cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga
     4021 ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca
     4081 gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgcccc ggctccgggc
     4141 gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga
     4201 tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt
     4261 cgggcgtaca caaatcgccc gcagaagcgc cgccgtctgg accgatggct gtgtagaagt
     4321 actcgccgat agtggaaacc gacgccccag cactcgtccg agggcaaagg aataagctag
     4381 tatgtaagcc tagtcttaga taataaaatc gctatccatc gaagatggat gtgtgttggt
     4441 tttttgtgtg tgtaacgcta ggcgcgcctg gtgtaccgag aacgatcctc tcagtgcgag
     4501 tctcgacgat ccatatcgtt gcttggcagt cagccagtcg gaatccagct tgggacccag
     4561 gaagtccaat cgtcagatat tgtactcaag cctggtcacg gcagcgtacc gatctgttta
     4621 aacctagata ttgatagtct gatcggtcaa cgtataatcg agtcctagct tttgcaaaca
     4681 tctatcaaga gacaggatca gcaggaggct ttcgcatgag tattcaacat ttccgtgtcg
     4741 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     4801 tgaaagtaaa agatgctgaa gatcagttgg gtgcgcgagt gggttacatc gaactggatc
     4861 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgctttcca atgatgagca
     4921 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     4981 tcggtcgccg catacactat tctcagaatg acttggttga gtattcacca gtcacagaaa
     5041 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     5101 ataacactgc ggccaactta cttctgacaa cgattggagg accgaaggag ctaaccgctt
     5161 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     5221 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaaccttgc
     5281 gtaaactatt aactggcgaa ctacttactc tagcttcccg gcaacagttg atagactgga
     5341 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     5401 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     5461 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     5521 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaaccga
     5581 ttctaggtgc attggcgcag aaaaaaatgc ctgatgcgac gctgcgcgtc ttatactccc
     5641 acatatgcca gattcagcaa cggatacggc ttccccaact tgcccacttc catacgtgtc
     5701 ctccttacca gaaatttatc cttaagatcc cgaatcgttt aaactcgact ctggctctat
     5761 cgaatctccg tcgtttcgag cttacgcgaa cagccgtggc gctcatttgc tcgtcgggca
     5821 tcgaatctcg tcagctatcg tcagcttacc tttttggcag cgatcgcggc tcccgacatc
     5881 ttggaccatt agctccacag gtatcttctt ccctctagtg gtcataacag cagcttcagc
     5941 tacctctcaa ttcaaaaaac ccctcaagac ccgtttagag gccccaaggg gttatgctat
     6001 caatcgttgc gttacacaca caaaaaacca acacacatcc atcttcgatg gatagcgatt
     6061 ttattatcta actgctgatc gagtgtagcc agatctagta atcaattacg gggtcattag
     6121 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     6181 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     6241 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     6301 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     6361 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     6421 tctacgtatt agtcatcgct attaccatgc tgatgcggtt ttggcagtac atcaatgggc
     6481 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     6541 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     6601 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag
     6661 tgaaccgtca gatcagatct ttgtcgatcc taccatccac tcgacacacc cgccagcggc
     6721 cgc
//
