LOCUS       sequence-297684-       11317 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  piggyBac transposon vector with CAG promoter expressing 
            SMARCA4-Venus fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11317)
  AUTHORS   Ballabio C, Anderle M, Gianesello M, Lago C, Miele E, Cardano M, 
            Aiello G, Piazza S, Caron D, Gianno F, Ciolfi A, Pedace L, 
            Mastronuzzi A, Tartaglia M, Locatelli F, Ferretti E, Giangaspero F, 
            Tiberi L
  TITLE     Modeling medulloblastoma in vivo and with human cerebellar 
            organoids.
  JOURNAL   Nat Commun. 2020 Jan 29;11(1):583. doi: 10.1038/s41467-019-13989-3.
  PUBMED    31996670
REFERENCE   2  (bases 1 to 11317)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-01-29"; volume: "11(1):583. doi"; pages: " 
            10.1038/s41467-019-13989-3"
COMMENT     Sequence Label: pPB CAG SMARCA4-Venus
FEATURES             Location/Qualifiers
     source          1..11317
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(96..887,888..956))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     719..738
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(957..1061)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1087..1542)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1224..1245)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1436..1455
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1669..1691
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1683..1700
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1684..1700
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1707..1726
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1707..1725
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   1744..1778
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        2061..2440
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2442..2719
                     /label=chicken beta-actin promoter
     intron          2720..3732
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3656..3678
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3740..3759
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             join(8669..8671,8672..9385)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(8711..8732)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8972..8991)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9319..9340
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(9479..9498)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    9544..9599
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9598..9617)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     repeat_region   10127..10189
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(10213..10231)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10252..10268)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10252..10268)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10265..10287)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10276..10292
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10300..10306,10307..10324,10325..10330))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10345..10366
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10483..10500)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10654..11242)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10734..10753)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
       61 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
      121 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
      181 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
      241 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
      301 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
      361 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
      421 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
      481 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
      541 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
      601 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
      661 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
      721 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
      781 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
      841 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
      901 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
      961 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     1021 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     1081 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     1141 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     1201 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     1261 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     1321 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     1381 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     1441 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     1501 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
     1561 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     1621 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     1681 gttgtaaaac gacggccagt gaattgtaat acgactcact atagggcgaa ttgggtacct
     1741 taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat attgctctct
     1801 ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg ccgcttggag
     1861 ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact ataacgaccg
     1921 cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta actcatacga
     1981 taattatatt gttatttcat gttctactta cgtgataact tattatatat atattttctt
     2041 gttatagata tcgtcgggtc gacattgatt attgactagt tattaatagt aatcaattac
     2101 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     2161 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     2221 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     2281 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     2341 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     2401 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt
     2461 ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt
     2521 ttaattattt tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc caggcggggc
     2581 ggggcggggc gaggggcggg gcggggcgag gcggagaggt gcggcggcag ccaatcagag
     2641 cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa
     2701 gcgaagcgcg cggcgggcgg gagtcgctgc gttgccttcg ccccgtgccc cgctccgcgc
     2761 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
     2821 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc tcgtttcttt
     2881 tctgtggctg cgtgaaagcc ttaaagggct ccgggagggc cctttgtgcg ggggggagcg
     2941 gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc ccgcgctgcc
     3001 cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgcg tgtgcgcgag
     3061 gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg ctgcgagggg aacaaaggct
     3121 gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt gggcgcggcg gtcgggctgt
     3181 aacccccccc tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg
     3241 ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg
     3301 gtgccgggcg gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc
     3361 ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa
     3421 tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg
     3481 aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag
     3541 gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag
     3601 cctcggggct gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc
     3661 ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc
     3721 tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa
     3781 gaattgcatg tccactccag acccacccct gggcggaact cctcggccag gtccttcccc
     3841 gggccctggc ccttcccctg gagccatgct gggccctagc ccgggtccct cgccgggctc
     3901 cgcccacagc atgatggggc ccagcccagg gccgccctca gcaggacacc ccatccccac
     3961 ccaggggcct ggagggtacc ctcaggacaa catgcaccag atgcacaagc ccatggagtc
     4021 catgcatgag aagggcatgt cggacgaccc gcgctacaac cagatgaaag gaatggggat
     4081 gcggtcaggg ggccatgctg ggatggggcc cccgcccagc cccatggacc agcactccca
     4141 aggttacccc tcgcccctgg gtggctctga gcatgcctct agtccagttc cagccagtgg
     4201 cccgtcttcg gggccccaga tgtcttccgg gccaggaggt gccccgctgg atggtgctga
     4261 cccccaggcc ttggggcagc agaaccgggg cccaacccca tttaaccaga accagctgca
     4321 ccagctcaga gctcagatca tggcctacaa gatgctggcc agggggcagc ccctccccga
     4381 ccacctgcag atggcggtgc agggcaagcg gccgatgccc gggatgcagc agcagatgcc
     4441 aacgctacct ccaccctcgg tgtccgcaac aggacccggc cctggccctg gccctggccc
     4501 cggcccgggt cccggcccgg cacctccaaa ttacagcagg cctcatggta tgggagggcc
     4561 caacatgcct cccccaggac cctcgggcgt gccccccggg atgccaggcc agcctcctgg
     4621 agggcctccc aagccctggc ctgaaggacc catggcgaat gctgctgccc ccacgagcac
     4681 ccctcagaag ctgattcccc cgcagccaac gggccgccct tcccccgcgc cccctgccgt
     4741 cccacccgcc gcctcgcccg tgatgccacc gcagacccag tcccccgggc agccggccca
     4801 gcccgcgccc atggtgccac tgcaccagaa gcagagccgc atcaccccca tccagaagcc
     4861 gcggggcctc gaccctgtgg agatcctgca ggagcgcgag tacaggctgc aggctcgcat
     4921 cgcacaccga attcaggaac ttgaaaacct tcccgggtcc ctggccgggg atttgcgaac
     4981 caaagcgacc attgagctca aggccctcag gctgctgaac ttccagaggc agctgcgcca
     5041 ggaggtggtg gtgtgcatgc ggagggacac agcgctggag acagccctca atgctaaggc
     5101 ctacaagcgc agcaagcgcc agtccctgcg cgaggcccgc atcactgaga agctggagaa
     5161 gcagcagaag atcgagcagg agcgcaagcg ccggcagaag caccaggaat acctcaatag
     5221 cattctccag catgccaagg atttcaagga atatcacaga tccgtcacag gcaaaatcca
     5281 gaagctgacc aaggcagtgg ccacgtacca tgccaacacg gagcgggagc agaagaaaga
     5341 gaacgagcgg atcgagaagg agcgcatgcg gaggctcatg gctgaagatg aggaggggta
     5401 ccgcaagctc atcgaccaga agaaggacaa gcgcctggcc tacctcttgc agcagacaga
     5461 cgagtacgtg gctaacctca cggagctggt gcggcagcac aaggctgccc aggtcgccaa
     5521 ggagaaaaag aagaaaaaga aaaagaagaa ggcagaaaat gcagaaggac agacgcctgc
     5581 cattgggccg gatggcgagc ctctggacga gaccagccag atgagcgacc tcccggtgaa
     5641 ggtgatccac gtggagagtg ggaagatcct cacaggcaca gatgccccca aagccgggca
     5701 gctggaggcc tggctcgaga tgaacccggg gtatgaagta gctccgaggt ctgatagtga
     5761 agaaagtggc tcagaagaag aggaagagga ggaggaggaa gagcagccgc aggcagcaca
     5821 gcctcccacc ctgcccgtgg aggagaagaa gaagattcca gatccagaca gcgatgacgt
     5881 ctctgaggtg gacgcgcggc acatcattga gaatgccaag caagatgtcg atgatgaata
     5941 tggcgtgtcc caggcccttg cacgtggcct gcagtcctac tatgccgtgg cccatgctgt
     6001 cactgagaga gtggacaagc agtcagcgct tatggtcaat ggtgtcctca aacagtacca
     6061 gatcaaaggt ttggagtggc tggtgtccct gtacaacaac aacctgaacg gcatcctggc
     6121 cgacgagatg ggcctgggga agaccatcca gaccatcgcg ctcatcacgt acctcatgga
     6181 gcacaaacgc atcaatgggc ccttcctcat catcgtgcct ctctcaacgc tgtccaactg
     6241 ggcgtacgag tttgacaagt gggccccctc cgtggtgaag gtgtcttaca agggatcccc
     6301 agcagcaaga cgggcctttg tcccccagct ccggagtggg aagttcaacg tcttgctgac
     6361 gacgtacgag tacatcatca aagacaagca catcctcgcc aagatccgtt ggaagtacat
     6421 gattgtggac gaaggtcacc gcatgaagaa ccaccactgc aagctgacgc aggtgctcaa
     6481 cacgcactat gtggcacccc gccgcctgct gctgacgggc acaccgctgc agaacaagct
     6541 tcccgagctc tgggcgctgc tcaacttcct gctgcccacc atcttcaaga gctgcagcac
     6601 cttcgagcag tggtttaacg caccctttgc catgaccggg gaaaaggtgg acctgaatga
     6661 ggaggaaacc attctcatca tccggcgtct ccacaaagtg ctgcggccct tcttgctccg
     6721 acgactcaag aaggaagtcg aggcccagtt gcccgaaaag gtggagtacg tcatcaagtg
     6781 cgacatgtct gcgctgcagc gagtgctcta ccgccacatg caggccaagg gcgtgctgct
     6841 gactgatggc tccgagaagg acaagaaggg caaaggcggc accaagaccc tgatgaacac
     6901 catcatgcag ctgcggaaga tctgcaacca cccctacatg ttccagcaca tcgaggagtc
     6961 cttttccgag cacttggggt tcactggcgg cattgtccaa gggctggacc tgtaccgagc
     7021 ctcgggtaaa tttgagcttc ttgatagaat tcttcccaaa ctccgagcaa ccaaccacaa
     7081 agtgctgctg ttctgccaaa tgacctccct catgaccatc atggaagatt actttgcgta
     7141 tcgcggcttt aaatacctca ggcttgatgg aaccacgaag gcggaggacc ggggcatgct
     7201 gctgaaaacc ttcaacgagc ccggctctga gtacttcatc ttcctgctca gcacccgggc
     7261 tggggggctc ggcctgaacc tccagtcggc agacactgtg atcatttttg acagcgactg
     7321 gaatcctcac caggacctgc aagcgcagga ccgagcccac cgcatcgggc agcagaacga
     7381 ggtgcgtgtg ctccgcctct gcaccgtcaa cagcgtggag gagaagatcc tagctgcagc
     7441 caagtacaag ctcaacgtgg accagaaggt gatccaggcc ggcatgttcg accagaagtc
     7501 ctccagccat gagcggcgcg ccttcctgca ggccatcctg gagcacgagg agcaggatga
     7561 ggaggaagac aaggtgcccg acgacgagac cgtcaaccag atgatcgccc ggcacgagga
     7621 ggagtttgat ctgttcatgc gcatggacct ggaccgcagg cgcgaggagg cccgcaaccc
     7681 caagcggaag ccgcgcctca tggaggagga cgagctcccc tcgtggatca tcaaggacga
     7741 cgcggaggtg gagcggctga cctgtgagga ggaggaggag aagatgttcg gccgtggctc
     7801 ccgccaccgc aaggaggtgg actacagcga ctcactgacg gagaagcagt ggctcaaggc
     7861 catcgaggag ggcacgctgg aggagatcga agaggaggtc cggcagaaga aatcatcacg
     7921 gaagcgcaag cgagacagcg acgccggctc ctccaccccg accaccagca cccgcagccg
     7981 cgacaaggac gacgagagca agaagcagaa gaagcgcggg cggccgcctg ccgagaaact
     8041 ctcccctaac ccacccaacc tcaccaagaa gatgaagaag attgtggatg ccgtgatcaa
     8101 gtacaaggac agcagcagtg gacgtcagct cagcgaggtc ttcatccagc tgccctcgcg
     8161 aaaggagctg cccgagtact acgagctcat ccgcaagccc gtggacttca agaagataaa
     8221 ggagcgcatt cgcaaccaca agtaccgcag cctcaacgac ctagagaagg acgtcatgct
     8281 cctgtgccag aacgcacaga ccttcaacct ggagggctcc ctgatctatg aagactccat
     8341 cgtcttgcag tcggtcttca ccagcgtgcg gcagaaaatc gagaaggagg atgacagtga
     8401 aggcgaggag agtgaggagg aggaagaggg cgaggaggaa ggctccgaat ccgaatctcg
     8461 gtccgtcaaa gtgaagatca agcttggccg gaaggagaag gcacaggacc agctgaaggg
     8521 cggccggcgg cggccgagcc gagggtcccg agccaagccg gtcgtgagtg acgatgacag
     8581 tgaggaggaa caagaggagg accgctcagg aagtggcagc gaagaagaca ccggtggttc
     8641 tggtggtggt tctggtggtg gttctggtgt gagcaagggc gaggagctgt tcaccggggt
     8701 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     8761 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagctgatct gcaccaccgg
     8821 caagctgccc gtgccctggc ccaccctcgt gaccaccctg ggctacggcc tgcagtgctt
     8881 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     8941 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     9001 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     9061 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     9121 tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat
     9181 cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     9241 ccccgtgctg ctgcccgaca accactacct gagctaccag tccaagctga gcaaagaccc
     9301 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     9361 cggcatggac gagctgtaca agtgatgagc ggccgcaatt cactcctcag gtgcaggctg
     9421 cctatcagaa ggtggtggct ggtgtggcca atgccctggc tcacaaatac cactgagatc
     9481 tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg
     9541 gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctatcac
     9601 tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga
     9661 gtttggcaac atatgcccat atgctggctg ccatgaacaa aggttggcta taaagaggtc
     9721 atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt
     9781 gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat
     9841 tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag
     9901 ctgtccctct tctcttatgg agatccctcg acctgcaccg tctatggaga tcggatcctt
     9961 tgttacttta tagaagaaat tttgagtttt tgtttttttt taataaataa ataaacataa
    10021 ataaattgtt tgttgaattt attattagta tgtaagtgta aatataataa aacttaatat
    10081 ctattcaaat taataaataa acctcgatat acagaccgat aaaacacatg cgtcaatttt
    10141 acgcatgatt atctttaacg tacgtcacaa tatgattatc tttctagggt taaagagctc
    10201 cagcttttgt tccctttagt gagggttaat ttcgagcttg gcgtaatcat ggtcatagct
    10261 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
    10321 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
    10381 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
    10441 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
    10501 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
    10561 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
    10621 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
    10681 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
    10741 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
    10801 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
    10861 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
    10921 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
    10981 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
    11041 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
    11101 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
    11161 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
    11221 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
    11281 gtggaacgaa aactcacgtt aagggatttt ggtcatg
//
