LOCUS       sequence-297687-       12451 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  piggyBac transposon vector with CAG promoter expressing wild-type 
            SMARCA4 (FLAG-tagged) and EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12451)
  AUTHORS   Ballabio C, Anderle M, Gianesello M, Lago C, Miele E, Cardano M, 
            Aiello G, Piazza S, Caron D, Gianno F, Ciolfi A, Pedace L, 
            Mastronuzzi A, Tartaglia M, Locatelli F, Ferretti E, Giangaspero F, 
            Tiberi L
  TITLE     Modeling medulloblastoma in vivo and with human cerebellar 
            organoids.
  JOURNAL   Nat Commun. 2020 Jan 29;11(1):583. doi: 10.1038/s41467-019-13989-3.
  PUBMED    31996670
REFERENCE   2  (bases 1 to 12451)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-01-29"; volume: "11(1):583. doi"; pages: " 
            10.1038/s41467-019-13989-3"
COMMENT     Sequence Label: pPB CAG SMARCA4-IRES-EGFP
FEATURES             Location/Qualifiers
     source          1..12451
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     588..610
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     602..619
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   711..773
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    1117..1150
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        1178..1557
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1559..1836
                     /label=chicken beta-actin promoter
     intron          1837..2849
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2773..2795
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2857..2876
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             7747..7770
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    join(7811..8385,8386..8388,8389..8397)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7978..7995)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8205..8224
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(8398..8400,8401..8403,8404..9117)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8398..8400,8401..8403,8404..9114)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8443..8464)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8704..8723)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9051..9072
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(9209..9228)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    9274..9329
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9328..9347)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     protein_bind    9676..9709
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     repeat_region   10076..10110
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(10260..10278)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10299..10315)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10299..10315)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10312..10334)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10323..10339
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10347..10353,10354..10371,10372..10377))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10392..10413
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10530..10547)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10701..11289)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10781..10800)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11460..12251,12252..12320))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12083..12102
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12321..12425)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcg cgccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcgg
      661 tattcacgac agcaggctga ataataaaaa aattagaaac tattatttaa ccctagaaag
      721 ataatcatat tgtgacgtac gttaaagata atcatgcgta aaattgacgc atgtgtttta
      781 tcggtctgta tatcgaggtt tatttattaa tttgaataga tattaagttt tattatattt
      841 acacttacat actaataata aattcaacaa acaatttatt tatgtttatt tatttattaa
      901 aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa aacttttaaa cattctctct
      961 tttacaaaaa taaacttatt ttgtacttta aaaacagtca tgttgtatta taaaataagt
     1021 aattagctta acctatacat aatagaaaca aattatactt attagtcagt cagaaacaac
     1081 tttggcacat atcaatatta tgctctcgtt aatcgcataa cttcgtataa tgtatgctat
     1141 acgaagttat aattcagatc tttcggcgcg ccgggtcgac attgattatt gactagttat
     1201 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     1261 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     1321 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     1381 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     1441 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     1501 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
     1561 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
     1621 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg gggggggggg
     1681 cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg
     1741 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
     1801 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgtt gccttcgccc
     1861 cgtgccccgc tccgcgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc
     1921 ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa
     1981 tgacggctcg tttcttttct gtggctgcgt gaaagcctta aagggctccg ggagggccct
     2041 ttgtgcgggg gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc
     2101 gtgcggcccg cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg
     2161 ctccgcgtgt gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc gggggggctg
     2221 cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg
     2281 cgcggcggtc gggctgtaac ccccccctgc acccccctcc ccgagttgct gagcacggcc
     2341 cggcttcggg tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg
     2401 ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg
     2461 gggaggggcg cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc
     2521 attgcctttt atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg
     2581 cggagccgaa atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt
     2641 gcggcgccgg caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc
     2701 cccttctccc tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac
     2761 ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc
     2821 atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt
     2881 ctcatcattt tggcaaagaa ttgcatgtcc actccagacc cacccctggg cggaactcct
     2941 cggccaggtc cttccccggg ccctggccct tcccctggag ccatgctggg ccctagcccg
     3001 ggtccctcgc cgggctccgc ccacagcatg atggggccca gcccagggcc gccctcagca
     3061 ggacacccca tccccaccca ggggcctgga gggtaccctc aggacaacat gcaccagatg
     3121 cacaagccca tggagtccat gcatgagaag ggcatgtcgg acgacccgcg ctacaaccag
     3181 atgaaaggaa tggggatgcg gtcagggggc catgctggga tggggccccc gcccagcccc
     3241 atggaccagc actcccaagg ttacccctcg cccctgggtg gctctgagca tgcctctagt
     3301 ccagttccag ccagtggccc gtcttcgggg ccccagatgt cttccgggcc aggaggtgcc
     3361 ccgctggatg gtgctgaccc ccaggccttg gggcagcaga accggggccc aaccccattt
     3421 aaccagaacc agctgcacca gctcagagct cagatcatgg cctacaagat gctggccagg
     3481 gggcagcccc tccccgacca cctgcagatg gcggtgcagg gcaagcggcc gatgcccggg
     3541 atgcagcagc agatgccaac gctacctcca ccctcggtgt ccgcaacagg acccggccct
     3601 ggccctggcc ctggccccgg cccgggtccc ggcccggcac ctccaaatta cagcaggcct
     3661 catggtatgg gagggcccaa catgcctccc ccaggaccct cgggcgtgcc ccccgggatg
     3721 ccaggccagc ctcctggagg gcctcccaag ccctggcctg aaggacccat ggcgaatgct
     3781 gctgccccca cgagcacccc tcagaagctg attcccccgc agccaacggg ccgcccttcc
     3841 cccgcgcccc ctgccgtccc acccgccgcc tcgcccgtga tgccaccgca gacccagtcc
     3901 cccgggcagc cggcccagcc cgcgcccatg gtgccactgc accagaagca gagccgcatc
     3961 acccccatcc agaagccgcg gggcctcgac cctgtggaga tcctgcagga gcgcgagtac
     4021 aggctgcagg ctcgcatcgc acaccgaatt caggaacttg aaaaccttcc cgggtccctg
     4081 gccggggatt tgcgaaccaa agcgaccatt gagctcaagg ccctcaggct gctgaacttc
     4141 cagaggcagc tgcgccagga ggtggtggtg tgcatgcgga gggacacagc gctggagaca
     4201 gccctcaatg ctaaggccta caagcgcagc aagcgccagt ccctgcgcga ggcccgcatc
     4261 actgagaagc tggagaagca gcagaagatc gagcaggagc gcaagcgccg gcagaagcac
     4321 caggaatacc tcaatagcat tctccagcat gccaaggatt tcaaggaata tcacagatcc
     4381 gtcacaggca aaatccagaa gctgaccaag gcagtggcca cgtaccatgc caacacggag
     4441 cgggagcaga agaaagagaa cgagcggatc gagaaggagc gcatgcggag gctcatggct
     4501 gaagatgagg aggggtaccg caagctcatc gaccagaaga aggacaagcg cctggcctac
     4561 ctcttgcagc agacagacga gtacgtggct aacctcacgg agctggtgcg gcagcacaag
     4621 gctgcccagg tcgccaagga gaaaaagaag aaaaagaaaa agaagaaggc agaaaatgca
     4681 gaaggacaga cgcctgccat tgggccggat ggcgagcctc tggacgagac cagccagatg
     4741 agcgacctcc cggtgaaggt gatccacgtg gagagtggga agatcctcac aggcacagat
     4801 gcccccaaag ccgggcagct ggaggcctgg ctcgagatga acccggggta tgaagtagct
     4861 ccgaggtctg atagtgaaga aagtggctca gaagaagagg aagaggagga ggaggaagag
     4921 cagccgcagg cagcacagcc tcccaccctg cccgtggagg agaagaagaa gattccagat
     4981 ccagacagcg atgacgtctc tgaggtggac gcgcggcaca tcattgagaa tgccaagcaa
     5041 gatgtcgatg atgaatatgg cgtgtcccag gcccttgcac gtggcctgca gtcctactat
     5101 gccgtggccc atgctgtcac tgagagagtg gacaagcagt cagcgcttat ggtcaatggt
     5161 gtcctcaaac agtaccagat caaaggtttg gagtggctgg tgtccctgta caacaacaac
     5221 ctgaacggca tcctggccga cgagatgggc ctggggaaga ccatccagac catcgcgctc
     5281 atcacgtacc tcatggagca caaacgcatc aatgggccct tcctcatcat cgtgcctctc
     5341 tcaacgctgt ccaactgggc gtacgagttt gacaagtggg ccccctccgt ggtgaaggtg
     5401 tcttacaagg gatccccagc agcaagacgg gcctttgtcc cccagctccg gagtgggaag
     5461 ttcaacgtct tgctgacgac gtacgagtac atcatcaaag acaagcacat cctcgccaag
     5521 atccgttgga agtacatgat tgtggacgaa ggtcaccgca tgaagaacca ccactgcaag
     5581 ctgacgcagg tgctcaacac gcactatgtg gcaccccgcc gcctgctgct gacgggcaca
     5641 ccgctgcaga acaagcttcc cgagctctgg gcgctgctca acttcctgct gcccaccatc
     5701 ttcaagagct gcagcacctt cgagcagtgg tttaacgcac cctttgccat gaccggggaa
     5761 aaggtggacc tgaatgagga ggaaaccatt ctcatcatcc ggcgtctcca caaagtgctg
     5821 cggcccttct tgctccgacg actcaagaag gaagtcgagg cccagttgcc cgaaaaggtg
     5881 gagtacgtca tcaagtgcga catgtctgcg ctgcagcgag tgctctaccg ccacatgcag
     5941 gccaagggcg tgctgctgac tgatggctcc gagaaggaca agaagggcaa aggcggcacc
     6001 aagaccctga tgaacaccat catgcagctg cggaagatct gcaaccaccc ctacatgttc
     6061 cagcacatcg aggagtcctt ttccgagcac ttggggttca ctggcggcat tgtccaaggg
     6121 ctggacctgt accgagcctc gggtaaattt gagcttcttg atagaattct tcccaaactc
     6181 cgagcaacca accacaaagt gctgctgttc tgccaaatga cctccctcat gaccatcatg
     6241 gaagattact ttgcgtatcg cggctttaaa tacctcaggc ttgatggaac cacgaaggcg
     6301 gaggaccggg gcatgctgct gaaaaccttc aacgagcccg gctctgagta cttcatcttc
     6361 ctgctcagca cccgggctgg ggggctcggc ctgaacctcc agtcggcaga cactgtgatc
     6421 atttttgaca gcgactggaa tcctcaccag gacctgcaag cgcaggaccg agcccaccgc
     6481 atcgggcagc agaacgaggt gcgtgtgctc cgcctctgca ccgtcaacag cgtggaggag
     6541 aagatcctag ctgcagccaa gtacaagctc aacgtggacc agaaggtgat ccaggccggc
     6601 atgttcgacc agaagtcctc cagccatgag cggcgcgcct tcctgcaggc catcctggag
     6661 cacgaggagc aggatgagga ggaagacaag gtgcccgacg acgagaccgt caaccagatg
     6721 atcgcccggc acgaggagga gtttgatctg ttcatgcgca tggacctgga ccgcaggcgc
     6781 gaggaggccc gcaaccccaa gcggaagccg cgcctcatgg aggaggacga gctcccctcg
     6841 tggatcatca aggacgacgc ggaggtggag cggctgacct gtgaggagga ggaggagaag
     6901 atgttcggcc gtggctcccg ccaccgcaag gaggtggact acagcgactc actgacggag
     6961 aagcagtggc tcaaggccat cgaggagggc acgctggagg agatcgaaga ggaggtccgg
     7021 cagaagaaat catcacggaa gcgcaagcga gacagcgacg ccggctcctc caccccgacc
     7081 accagcaccc gcagccgcga caaggacgac gagagcaaga agcagaagaa gcgcgggcgg
     7141 ccgcctgccg agaaactctc ccctaaccca cccaacctca ccaagaagat gaagaagatt
     7201 gtggatgccg tgatcaagta caaggacagc agcagtggac gtcagctcag cgaggtcttc
     7261 atccagctgc cctcgcgaaa ggagctgccc gagtactacg agctcatccg caagcccgtg
     7321 gacttcaaga agataaagga gcgcattcgc aaccacaagt accgcagcct caacgaccta
     7381 gagaaggacg tcatgctcct gtgccagaac gcacagacct tcaacctgga gggctccctg
     7441 atctatgaag actccatcgt cttgcagtcg gtcttcacca gcgtgcggca gaaaatcgag
     7501 aaggaggatg acagtgaagg cgaggagagt gaggaggagg aagagggcga ggaggaaggc
     7561 tccgaatccg aatctcggtc cgtcaaagtg aagatcaagc ttggccggaa ggagaaggca
     7621 caggaccagc tgaagggcgg ccggcggcgg ccgagccgag ggtcccgagc caagccggtc
     7681 gtgagtgacg atgacagtga ggaggaacaa gaggaggacc gctcaggaag tggcagcgaa
     7741 gaagacgact acaaggacga cgatgacaaa tgaaattctg cagtcgacgg taccgcgggc
     7801 ccgggatccg cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat
     7861 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
     7921 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
     7981 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
     8041 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
     8101 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
     8161 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
     8221 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
     8281 ggcctcggta cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc
     8341 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatggc cacaaccatg
     8401 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     8461 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     8521 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     8581 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
     8641 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     8701 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     8761 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     8821 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     8881 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     8941 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     9001 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     9061 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaaagc
     9121 ggccgcaatt cactcctcag gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca
     9181 atgccctggc tcacaaatac cactgagatc tttttccctc tgccaaaaat tatggggaca
     9241 tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa
     9301 tagtgtgttg gaattttttg tgtctatcac tcggaaggac atatgggagg gcaaatcatt
     9361 taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat atgctggctg
     9421 ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc
     9481 attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg
     9541 tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt
     9601 tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatgg agatccctcg
     9661 acctgcaccg tcgagataac ttcgtataat gtatgctata cgaagttatg ctagccaaca
     9721 agctcgtcat cgctttgcag aagagcagag aggatatgct catcgtctaa agaactaccc
     9781 attttattat atattagtca cgatatctat aacaagaaaa tatatatata ataagttatc
     9841 acgtaagtag aacatgaaat aacaatataa ttatcgtatg agttaaatct taaaagtcac
     9901 gtaaaagata atcatgcgtc attttgactc acgcggtcgt tatagttcaa aatcagtgac
     9961 acttaccgca ttgacaagca cgcctcacgg gagctccaag cggcgactga gatgtcctaa
    10021 atgcacagcg acggattcgc gctatttaga aagagagagc aatatttcaa gaatgcatgc
    10081 gtcaatttta cgcagactat ctttctaggg ttaaaaaaga tttgcgcttt actcgaccta
    10141 aactttaaac acgtcataga atcttcgttt gacaaaaacc acattgtggc caagctgtgt
    10201 gacgcgacgc gcgctaaaga atggcaaacc aagtcgcgcg aggtacccag cttttgttcc
    10261 ctttagtgag ggttaattcc gagcttggcg taatcatggt catagctgtt tcctgtgtga
    10321 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
    10381 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
    10441 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
    10501 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
    10561 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
    10621 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
    10681 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
    10741 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
    10801 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
    10861 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
    10921 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
    10981 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
    11041 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
    11101 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
    11161 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
    11221 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
    11281 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
    11341 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
    11401 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
    11461 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
    11521 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
    11581 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
    11641 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
    11701 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
    11761 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
    11821 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
    11881 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
    11941 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
    12001 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
    12061 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    12121 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    12181 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    12241 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    12301 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    12361 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    12421 ccgcgcacat ttccccgaaa agtgccacct g
//
