LOCUS       sequence-297729-        9312 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression 
            and incorporation of photocaged-lysine; for transient or stable 
            piggyBac-mediated integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9312)
  AUTHORS   Meineke B, Heimgartner J, Caridha R, Block MF, Kimler KJ, Pires MF, 
            Landreh M, Elsasser SJ
  TITLE     Dual stop codon suppression in mammalian cells with genomically 
            integrated genetic code expansion machinery.
  JOURNAL   Cell Rep Methods. 2023 Nov 20;3(11):100626. doi: 
            10.1016/j.crmeth.2023.100626. Epub 2023 Nov 6.
  PUBMED    37935196
REFERENCE   2  (bases 1 to 9312)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Methods."; date: "2023-11-20"; volume: "3(11):100626. doi"; pages: "
            10.1016/j.crmeth.2023.100626. Epub 2023 Nov 6"
COMMENT     Sequence Label: pAS_4xMma PylT_FLAG-PcKRS
FEATURES             Location/Qualifiers
     source          1..9312
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        2727..2938
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2951..3219
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      3251..3260
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3260..3283
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    join(4562..5136,5137..5139,5140..5148)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4729..4746)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4956..4975
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5161..5760
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5161..5180)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5657..5677
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(5820..5840)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    5882..6003
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5919..5938)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5973..5992
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   6766..6800
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7291..7307)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7291..7307)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7304..7326)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7315..7331
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7339..7345,7346..7363,7364..7369))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7384..7405
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7522..7539)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7693..8281)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7773..7792)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8452..9243,9244..9312))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9075..9094
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccaact agtgaaaaac cgcacttgtc
     1381 cggaaacccc gggaatctaa cccggctgaa cggatttaga gtccgttcga tctacatgat
     1441 caggtttccg aggtacccag gcggcgcaca agctatataa acctgaagga aatctcaact
     1501 ttacacttag gtcaagttac ttatcgtact agagcttcag caggaaattt aactaaaatc
     1561 taatttaacc agcatagcaa atatcattta ttcccaaaat gctaaagttt gagataaacg
     1621 gacttgattt ccggctgttt tgacactatc cagaatgcct tgcagatggg tggggcatgc
     1681 taaatactgc aggaaaaacc gcacttgtcc ggaaaccccg ggaatctaac ccggctgaac
     1741 ggatttagag tccgttcgat ctacatgatc aggtttccga ggtacccagg cggcgcacaa
     1801 gctatataaa cctgaaggaa atctcaactt tacacttagg tcaagttact tatcgtacta
     1861 gagcttcagc aggaaattta actaaaatct aatttaacca gcatagcaaa tatcatttat
     1921 tcccaaaatg ctaaagtttg agataaacgg acttgatttc cggctgtttt gacactatcc
     1981 agaatgcctt gcagatgggt ggggcatgct aaatactgca ggaaaaaccg cacttgtccg
     2041 gaaaccccgg gaatctaacc cggctgaacg gatttagagt ccgttcgatc tacatgatca
     2101 ggtttccgag gtacccaggc ggcgcacaag ctatataaac ctgaaggaaa tctcaacttt
     2161 acacttaggt caagttactt atcgtactag agcttcagca ggaaatttaa ctaaaatcta
     2221 atttaaccag catagcaaat atcatttatt cccaaaatgc taaagtttga gataaacgga
     2281 cttgatttcc ggctgttttg acactatcca gaatgccttg cagatgggtg gggcatgcta
     2341 aatactgcag gaaaaaccgc acttgtccgg aaaccccggg aatctaaccc ggctgaacgg
     2401 atttagagtc cgttcgatct acatgatcag gtttccgagg tacccaggcg gcgcacaagc
     2461 tatataaacc tgaaggaaat ctcaacttta cacttaggtc aagttactta tcgtactaga
     2521 gcttcagcag gaaatttaac taaaatctaa tttaaccagc atagcaaata tcatttattc
     2581 ccaaaatgct aaagtttgag ataaacggac ttgatttccg gctgttttga cactatccag
     2641 aatgccttgc agatgggtgg ggcatgctaa atactgcaga actagtgctc tagaaaggat
     2701 ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
     2761 gttgggggga ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg
     2821 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
     2881 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct
     2941 gaagcttcga ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca
     3001 tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc
     3061 cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg
     3121 gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta
     3181 cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac
     3241 tctagagcta gccaccatgg actacaagga cgacgacgac aaggacaaga aacccctgga
     3301 cgtgctgatc agcgccaccg gcctgtggat gagccggacc ggcaccctgc acaagatcaa
     3361 gcaccacgag gtgtcaagaa gcaaaatcta catcgagatg gcctgcggcg accacctggt
     3421 ggtgaacaac agcagaagct gccggaccgc cagagccttc cggcaccaca agtacagaaa
     3481 gacctgtaag cggtgccggg tgtccgacga ggacatcaac aactttctga ccagaagcac
     3541 cgagagcaag aacagcgtga aagtgcgggt ggtgtccgcc cccaaagtga agaaagccat
     3601 gcccaagagc gtgtccagag cccccaagcc cctggaaaac agcgtgtccg ccaaggccag
     3661 caccaacacc agccgcagcg tgcccagccc cgccaagagc acccccaaca gctccgtgcc
     3721 cgcctctgct cctgctccca gcctgacacg gtcccagctg gacagagtgg aggccctgct
     3781 gtcccccgag gacaagatca gcctgaacat ggccaagccc ttccgggagc tggaacccga
     3841 gctggtgacc cggcggaaga acgacttcca gcggctgtac accaacgacc gggaggacta
     3901 cctgggcaag ctggaacggg acatcaccaa gttcttcgtg gaccggggct tcctggaaat
     3961 caagagcccc atcctgatcc ccgccgagta cgtggagcgg ttcggcatca acaacgacac
     4021 cgagctgtcc aagcagattt tccgggtgga caagaacctg tgcctgcggc ctatgctgag
     4081 ccccaccctg tgcaactaca tgcggaaact ggacagaatc ctgcctggcc ccatcaagat
     4141 tttcgaagtg ggaccctgct accggaaaga gagcgacggc aaagagcacc tggaagagtt
     4201 tacaatggtg aatttttgcc agatgggcag cggctgcacc cgggagaacc tggaagccct
     4261 gatcaaagag ttcctggatt acctggaaat cgacttcgag atcgtgggcg acagctgcat
     4321 ggtgtacggc gacaccctgg acatcatgca cggcgacctg gaactgagca gcgccgtggt
     4381 gggacccgtg tccctggacc gggagtgggg catcgacaag ccctggatcg gagccggctt
     4441 cggcctggaa cggctgctga aagtgatgca cggcttcaag aacatcaagc gggccagcag
     4501 aagcgagagc tactacaacg gcatcagcac caacctgtga tgataaggat ccgcggccgc
     4561 gcccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt
     4621 gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc
     4681 ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag
     4741 gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac
     4801 aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc
     4861 tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc
     4921 acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca
     4981 aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt
     5041 gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg
     5101 gggacgtggt tttcctttga aaaacacgat gataatatgg ccacaaccat ggcgtccgga
     5161 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     5221 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     5281 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     5341 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     5401 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     5461 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     5521 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     5581 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     5641 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     5701 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
     5761 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     5821 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgcgttaact
     5881 aaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     5941 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     6001 ttatcatgtc tggaattgac tcaaatgatg tcaattagtc tatcagaagc tcatctggtc
     6061 tcccttccgg gggacaagac atccctgttt aatatttaaa cagcagtgtt cccaaactgg
     6121 gttcttatat cccttgctct ggtcaaccag gttgcagggt ttcctgtcct cacaggaacg
     6181 aagtccctaa agaaacagtg gcagccaggt ttagccccgg aattgactgg attccttttt
     6241 tagggcccat tggtatggct ttttccccgt atccccccag gtgtctgcag gctcaaagag
     6301 cagcgagaag cgttcagagg aaagcgatcc cgtgccacct tccccgtgcc cgggctgtcc
     6361 ccgcacgctg ccggctcggg gatgcggggg gagcgccgga ccggagcgga gccccgggcg
     6421 gctcgctgct gccccctagc gggggaggga cgtaattaca tccctggggg ctttgggggg
     6481 gggctgtccc tgatatctat aacaagaaaa tatatatata ataagttatc acgtaagtag
     6541 aacatgaaat aacaatataa ttatcgtatg agttaaatct taaaagtcac gtaaaagata
     6601 atcatgcgtc attttgactc acgcggtcgt tatagttcaa aatcagtgac acttaccgca
     6661 ttgacaagca cgcctcacgg gagctccaag cggcgactga gatgtcctaa atgcacagcg
     6721 acggattcgc gctatttaga aagagagagc aatatttcaa gaatgcatgc gtcaatttta
     6781 cgcagactat ctttctaggg ttaatctagc tgcatcagga tcatatcgtc gggtcttttt
     6841 tccggctcag tcatcgccca agctggcgct atctgggcat cggggaggaa gaagcccgtg
     6901 ccttttcccg cgaggttgaa gcggcatgga aagagtttgc cgaggatgac tgctgctgca
     6961 ttgacgttga gcgaaaacgc acgtttacca tgatgattcg ggaaggtgtg gccatgcacg
     7021 cctttaacgg tgaactgttc gttcaggcca cctgggatac cagttcgtcg cggcttttcc
     7081 ggacacagtt ccggatggtc agcccgaagc gcatcagcaa cccgaacaat accggcgaca
     7141 gccggaactg ccgtgccggt gtgcagatta atgacagcgg tgcggcgctg ggatattacg
     7201 tcagcgagga cgggtatcct ggctggatgc cgcagaaatg gacatggata ccccgtgagt
     7261 tacccggcgg gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     7321 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     7381 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     7441 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     7501 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     7561 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     7621 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     7681 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     7741 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     7801 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     7861 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     7921 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     7981 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     8041 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     8101 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     8161 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8221 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8281 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8341 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8401 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     8461 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     8521 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     8581 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     8641 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     8701 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     8761 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     8821 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     8881 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     8941 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     9001 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     9061 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     9121 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     9181 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     9241 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9301 ttgaatactc at
//
