LOCUS       sequence-297771-        6170 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast Expression plasmid for SARS-CoV-2 ORF7a protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6170)
  TITLE     SARS-CoV-2 protein Yeast expression constructs, tagged, for protein 
            expression and/or purification
REFERENCE   2  (bases 1 to 6170)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4046221
FEATURES             Location/Qualifiers
     source          1..6170
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        4..417
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             794..811
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(1954..1972)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             2323..3126
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      3209..4162
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     terminator      4240..4269
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      4291..4878
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(4890..4917)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(4941..5132)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(5133..5924,5925..5993))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5756..5775
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5994..6096)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(6097..6128)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 gcggccgccc cacacaccat agcttcaaaa tgtttctact ccttttttac tcttccagat
       61 tttctcggac tccgcgcatc gccgtaccac ttcaaaacac ccaagcacag catactaaat
      121 ttcccctctt tcttcctcta gggtgtcgtt aattacccgt actaaaggtt tggaaaagaa
      181 aaaagagacc gcctcgtttc tttttcttcg nngaaaaagg caataaaaat ttttatcacg
      241 tttctttttc ttgaaaattt ttttttttga tttttttctc tttcgatgac ctcccattga
      301 tatttaagtt aataaacggt cttcaatttc tcaagtttca gtttcatttt tcttgttcta
      361 ttacaacttt ttttacttct tgctcattag aaagaaagca tagcaatcta atctaagttt
      421 taattacaaa atgaaaatta ttcttttctt ggcactgata acactcgcta cttgtgagct
      481 ttatcactac caagagtgtg ttagaggtac aacagtactt ttaaaagaac cttgctcttc
      541 tggaacatac gagggcaatt caccatttca tcctctagct gataacaaat ttgcactgac
      601 ttgctttagc actcaatttg cttttgcttg tcctgacggc gtaaaacacg tctatcagtt
      661 acgtgccaga tcagtttcac ctaaactgtt catcagacaa gaggaagttc aagaacttta
      721 ctctccaatt tttcttattg ttgcggcaat agtgtttata acactttgct tcacactcaa
      781 aagaaagaca gaactcgtgc cgagaggttc taaaatcgag gaaggtaagc tagtcatttg
      841 gataaatgga gataagggtt acaacggttt ggcagaagta ggcaagaaat ttgaaaagga
      901 cacaggtatc aaggtgacgg ttgaacatcc agacaagtta gaagaaaaat tccctcaagt
      961 tgctgctact ggtgatggtc ccgacattat tttctgggcc cacgatcgtt ttggtggata
     1021 tgctcagtca ggtttgctgg ctgagatcac cccagacaag gccttccaag ataaattgta
     1081 cccattcact tgggatgctg tcagatacaa cggtaagctt atagcatatc caattgctgt
     1141 tgaagcctta tcgttgatct acaataagga cttgttacct aacccaccaa agacatggga
     1201 agaaattcca gctttggata aagagctaaa ggccaaggga aaaagcgctt tgatgtttaa
     1261 cttacaagaa ccttatttca cctggccatt aatcgctgct gacggtggtt acgcatttaa
     1321 atacgaaaac ggtaagtatg acattaagga tgtcggcgtt gataatgctg gtgcaaaggc
     1381 tggtttgacc ttcttggttg atttaataaa gaataaacac atgaacgctg acactgacta
     1441 ctctatcgcc gaagccgctt tcaacaaggg tgaaactgct atgaccataa atggtccatg
     1501 ggcctggagt aacattgata cttctaaagt aaactatggt gtcactgttt tgcctacatt
     1561 caaaggccaa ccatccaagc cctttgtcgg tgttctatcc gctggcatca atgctgcttc
     1621 tccaaacaag gagctagcta aagaattttt ggaaaattac ttgttaactg acgaaggttt
     1681 ggaggccgtt aacaaggaca agccattggg tgctgtcgct ttgaaatctt acgaagaaga
     1741 attggctaag gatcccagaa ttgccgctac catggagaac gcccaaaagg gtgaaattat
     1801 gccaaacatc ccacaaatgt cagccttctg gtacgcagtt aggactgccg tgattaatgc
     1861 tgcttccggt agacaaaccg tcgacgaagc attaaaagat gctcaaacgt aaacaggccc
     1921 cttttccttt gtcgatatca tgtaattagt tatgtcacgc ttacattcac gccctcctcc
     1981 cacatccgct ctaaccgaaa aggaaggagt tagacaacct gaagtctagg tccctattta
     2041 ttttttttaa tagttatgtt agtattaaga acgttattta tatttcaaat ttttcttttt
     2101 tttctgtaca aagcggccgc cgaaccacta gtgcactgca gtacacggtt tccttgaaat
     2161 ttttttgatt cggtaatctc cgaacagaag gaagaacgaa ggaaggagca cagacttaga
     2221 ttggtatata tacgcatatg tagtgttgaa gaaacatgaa attgcccagt attcttaacc
     2281 caactgcaca gaacaaaaac gtgcaggaaa cgaagataaa tcatgtcgaa agctacatat
     2341 aaggaacgtg ctgctactca tcctagtcct gttgctgcca agctatttaa tatcatgcac
     2401 gaaaagcaaa caaacttgtg tgcttcattg gatgttcgta ccaccaagga attactggag
     2461 ttagttgaag cattaggtcc caaaatttgt ttactaaaaa cacatgtgga tatcttgact
     2521 gatttttcca tggagggcac agttaagccg ctaaaggcat tatccgccaa gtacaatttt
     2581 ttactcttcg aggacagaaa atttgctgac attggtaata cagtcaaatt gcagtactct
     2641 gcgggtgtat acagaatagc agaatgggca gacattacga atgcacacgg tgtggtgggc
     2701 ccaggtattg ttagcggttt gaagcaggcg gcagaagaag taacaaagga acctagaggc
     2761 cttttgatgt tagcagaatt gtcatgcaag ggctccctat ctactggaga atatactaag
     2821 ggtactgttg acattgcgaa gagcgacaaa gattttgtta tcggctttat tgctcaaaga
     2881 gacatgggtg gaagagatga aggttacgat tggttgatta tgacacccgg tgtgggttta
     2941 gatgacaagg gagatgcatt gggtcaacag tatagaaccg tggatgatgt ggtttctaca
     3001 ggatctgaca ttattattgt tggaagagga ctatttgcaa agggaaggga tgctaaggta
     3061 gagggtgaac gttacagaaa agcaggctgg gaagcatatt tgagaagatg cggccagcaa
     3121 aactaaaaaa ctgtattata agtaaatgca tgtatactaa actcacaaat tagagcttca
     3181 atttaattat atcagttatt acccgagtaa cgaagcatct gtgcttcatt ttgtagaaca
     3241 aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca tttttacaga
     3301 acagaaatgc aacgcgaaag cgctatttta ccaacgaaga atctgtgctt catttttgta
     3361 aaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa agaatctgag ctgcattttt
     3421 acagaacaga aatgcaacgc gagagcgcta ttttaccaac aaagaatcta tacttctgcg
     3481 ctatttttct aacaaagcat cttagattac tttttttctc ctttgtgcgc tctataatgc
     3541 agtctcttga taactttttg cactgtaggt ccgttaaggt tagaagaagg ctactttggt
     3601 gtctattttc tcttccataa aaaaagcctg actccacttc ccgcgtttac tgattactag
     3661 cgaagctgcg ggtgcatttt ttcaagataa aggcatcccc gattatattc tataccgatg
     3721 tggattgcgc atactttgtg aacagaaagt gatagcgttg atgattcttc attggtcaga
     3781 aaattatgaa cggtttcttc tattttgtct ctatatacta cgtataggaa atgtttacat
     3841 tttcgtattg ttttcgattc actctatgaa tagttcttac tacaattttt ttgtctaaag
     3901 agtaatacta gagataaaca taaaaaatgt agaggtcgag tttagatgca agttcaagga
     3961 gcgaaaggtg gatgggtagg ttatataggg atatagcaca gagatatata gcaaagagat
     4021 acttttgagc aatgtttgtg gaagcggtat tcgcaatatt ttagtagctc gttacagtcc
     4081 ggtgcgtttt tggttttttg aaagtgcgtc atcagagcgc ttttggtttt caaaagcgct
     4141 ctgaagttcc tatactttct agctagagaa taggaacttc ccgagcggcc gcgtgttaca
     4201 agcttgactc cagcgtaact ggactgcaat caactcactg gctcaccttc acgggtgggc
     4261 ctttcttcgg tagaaaatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     4321 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     4381 accaactctt tttccgaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     4441 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     4501 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     4561 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     4621 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     4681 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     4741 agggtcggaa caggagagcg cacgagggag cttccagggg taaacgcctg gtatctttat
     4801 agtcctgtcg ggtttcgcca cctctgactt gagcatcgat ttttgtgatg ctcgtcaggg
     4861 gggcggagcc tatggaaaaa cgccagcaac gcagaaaggc ccacccgaag gtgagccagg
     4921 tgattacatt tgggccctca tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag
     4981 ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc
     5041 gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc
     5101 atgttaaggg cggttttttc ctgtttggtc acttaccaat gcttaatcag tgaggcacct
     5161 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     5221 actacgatgc gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     5281 cgctcaccgg ctccagattt atcagcaata aaccagccag ccgggagggc cgagcgcaga
     5341 agtgatcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     5401 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     5461 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     5521 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     5581 gccagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     5641 cttactgtca tgccatccgt gagatgcttt tctgtgactg gtgagtactc aaccaagtca
     5701 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     5761 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcgt
     5821 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     5881 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     5941 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact cattttagct
     6001 tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca
     6061 ttatggtgaa agttggaacc tcttacgtgc cgatcaagtc aaaagcctcc ggtcggaggc
     6121 ttttgacttt ctgctatgga ggtcaggtat gatttaaatg gtcagttaga
//
