LOCUS       sequence-297775-        9417 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid with 4xMmaPylT cassette and MmaPylRS AF for amber 
            suppression; for transient or stable piggyBac-mediated integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9417)
  AUTHORS   Meineke B, Heimgartner J, Lafranchi L, Elsasser SJ
  TITLE     Methanomethylophilus alvus Mx1201 provides basis for mutual 
            orthogonal pyrrolysyl tRNA/aminoacyl-tRNA synthetase pairs in 
            mammalian cells.
  JOURNAL   ACS Chem Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571.
  PUBMED    30260624
REFERENCE   2  (bases 1 to 9417)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571."
COMMENT     Sequence Label: pAS_4xMma PylT_FLAG-Mma PylRS AF
FEATURES             Location/Qualifiers
     source          1..9417
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     39..61
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     53..70
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     54..70
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   200..262
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        2053..2264
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2277..2545
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      2577..2586
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2586..2609
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    join(3993..4567,4568..4570,4571..4579)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4160..4177)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4387..4406
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4592..5191
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4592..4611)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5088..5108
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(5251..5271)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    5313..5434
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5350..5369)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5404..5423
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   6197..6231
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6722..6738)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6722..6738)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6735..6757)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6746..6762
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6770..6776,6777..6794,6795..6800))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6815..6836
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6953..6970)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7124..7712)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7204..7223)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7883..8674,8675..8743))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8506..8525
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8744..8848)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8874..9329)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9011..9032)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9223..9242
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
       61 gacggccagt gagcgcgcct cgttcattca cgtttttgaa cccgtggagg acgggcagac
      121 tcgcggtgca aatgtgtttt acagcgtgat ggagcagatg aagatgctcg acacgctgca
      181 gaacacgcag ctagattaac cctagaaaga taatcatatt gtgacgtacg ttaaagataa
      241 tcatgtgtaa aattgacgca tgtgttttat cggtctgtat atcgaggttt atttattaat
      301 ttgaatagat attaagtttt attatattta cacttacata ctaataataa attcaacaaa
      361 caatttattt atgtttattt atttattaaa aaaaacaaaa actcaaaatt tcttctataa
      421 agtaacaaaa cttttatgag ggacagcccc cccccaaagc ccccagggat gtaattacgt
      481 ccctcccccg ctagggggca gcagcgagcc gcccggggct ccgctccggt ccggcgctcc
      541 ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg gcacggggaa ggtggcacgg
      601 gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc tgcagacacc tggggggata
      661 cggggaaaag gcctccacgg ccaactagtg aaaaaccgca cttgtccgga aaccccggga
      721 atctaacccg gctgaacgga tttagagtcc gttcgatcta catgatcagg tttccgaggt
      781 acccaggcgg cgcacaagct atataaacct gaaggaaatc tcaactttac acttaggtca
      841 agttacttat cgtactagag cttcagcagg aaatttaact aaaatctaat ttaaccagca
      901 tagcaaatat catttattcc caaaatgcta aagtttgaga taaacggact tgatttccgg
      961 ctgttttgac actatccaga atgccttgca gatgggtggg gcatgctaaa tactgcagga
     1021 aaaaccgcac ttgtccggaa accccgggaa tctaacccgg ctgaacggat ttagagtccg
     1081 ttcgatctac atgatcaggt ttccgaggta cccaggcggc gcacaagcta tataaacctg
     1141 aaggaaatct caactttaca cttaggtcaa gttacttatc gtactagagc ttcagcagga
     1201 aatttaacta aaatctaatt taaccagcat agcaaatatc atttattccc aaaatgctaa
     1261 agtttgagat aaacggactt gatttccggc tgttttgaca ctatccagaa tgccttgcag
     1321 atgggtgggg catgctaaat actgcaggaa aaaccgcact tgtccggaaa ccccgggaat
     1381 ctaacccggc tgaacggatt tagagtccgt tcgatctaca tgatcaggtt tccgaggtac
     1441 ccaggcggcg cacaagctat ataaacctga aggaaatctc aactttacac ttaggtcaag
     1501 ttacttatcg tactagagct tcagcaggaa atttaactaa aatctaattt aaccagcata
     1561 gcaaatatca tttattccca aaatgctaaa gtttgagata aacggacttg atttccggct
     1621 gttttgacac tatccagaat gccttgcaga tgggtggggc atgctaaata ctgcaggaaa
     1681 aaccgcactt gtccggaaac cccgggaatc taacccggct gaacggattt agagtccgtt
     1741 cgatctacat gatcaggttt ccgaggtacc caggcggcgc acaagctata taaacctgaa
     1801 ggaaatctca actttacact taggtcaagt tacttatcgt actagagctt cagcaggaaa
     1861 tttaactaaa atctaattta accagcatag caaatatcat ttattcccaa aatgctaaag
     1921 tttgagataa acggacttga tttccggctg ttttgacact atccagaatg ccttgcagat
     1981 gggtggggca tgctaaatac tgcagaacta gtgctctaga aaggatctgc gatcgctccg
     2041 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg
     2101 tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg
     2161 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg
     2221 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag cttcgagggg
     2281 ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca cgccggttga
     2341 gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa
     2401 gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc ctacctagac
     2461 tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc tttgtttcgt
     2521 tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctactcta gagctagcca
     2581 ccatggacta caaggacgac gacgacaaga tggacaaaaa accgctgaat accctgatct
     2641 ctgctactgg tctgtggatg agtcgtaccg gaaccattca taaaatcaaa caccacgagg
     2701 ttagccgttc gaaaatctat attgagatgg cgtgtggcga tcatctggtt gtgaacaata
     2761 gccgctcttc tcgtacagca cgtgcactgc gtcaccacaa atatcgtaaa acctgtaaac
     2821 gttgccgtgt gtccgatgag gatctgaaca aattcctgac aaaagccaat gaggaccaaa
     2881 caagcgtgaa agtgaaagtc gttagcgctc ctacccgtac taaaaaagca atgccgaaat
     2941 ccgttgctcg tgcccctaaa ccactggaaa acactgaagc agcacaggca cagccgtctg
     3001 gaagcaaatt ctctccggcc attcctgttt ctacccagga gtccgtttct gttccagcaa
     3061 gtgtgagcac cagcattagc agtattagca ccggtgccac cgctagcgcc ctggttaaag
     3121 gcaataccaa tccgattaca agcatgtctg ccccggttca agcatcagct ccagcactga
     3181 caaaatccca aaccgatcgt ctggaggttc tgctgaatcc gaaagacgaa atcagcctga
     3241 attccggcaa accgtttcgt gaactggaga gcgaactgct gtcacgtcgt aaaaaagacc
     3301 tgcaacaaat ctatgccgaa gaacgtgaga actatctggg gaaactggaa cgtgaaatca
     3361 cccgcttttt cgtggatcgt ggctttctgg agatcaaatc cccgattctg attcctctgg
     3421 agtatatcga gcgtatgggc atcgacaatg ataccgaact gagcaaacaa attttccgtg
     3481 tggataaaaa cttctgtctg cgccctatgc tagcaccaaa tctggctaac tatctgcgca
     3541 aactggaccg tgccctgcct gatcctatca aaatcttcga gatcggcccg tgttatcgta
     3601 aagagtccga cggtaaagaa catctggagg agtttaccat gctgaacttt tgccaaatgg
     3661 gttcaggttg tactcgtgag aacctggaaa gcatcatcac cgattttctg aaccacctgg
     3721 gcattgactt caaaattgtg ggcgacagct gtatggtgtt tggcgacacc ctggatgtca
     3781 tgcacggcga cctggaactg tctagtgccg ttgttggacc aattccgctg gaccgtgagt
     3841 ggggtatcga caaaccgtgg atcggagcag gattcggtct ggaacgcctg ctgaaagtga
     3901 aacacgactt caaaaacatc aaacgtgccg cccgttctga atcgtattat aacgggatct
     3961 ctacgaacct gtaataagga tccgcggccg cgcccctctc cctccccccc ccctaacgtt
     4021 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc
     4081 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc
     4141 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag
     4201 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg
     4261 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat
     4321 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga
     4381 gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc
     4441 cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg
     4501 ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
     4561 tgataatatg gccacaacca tggcgtccgg aatgaccgag tacaagccca cggtgcgcct
     4621 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     4681 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     4741 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     4801 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     4861 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     4921 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     4981 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     5041 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     5101 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     5161 ctggtgcatg acccgcaagc ccggtgcctg agtcgacaat caacctctgg attacaaaat
     5221 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     5281 tgctttaatg cctttgtatc atgcgttaac taaacttgtt tattgcagct tataatggtt
     5341 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     5401 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggaattga ctcaaatgat
     5461 gtcaattagt ctatcagaag ctcatctggt ctcccttccg ggggacaaga catccctgtt
     5521 taatatttaa acagcagtgt tcccaaactg ggttcttata tcccttgctc tggtcaacca
     5581 ggttgcaggg tttcctgtcc tcacaggaac gaagtcccta aagaaacagt ggcagccagg
     5641 tttagccccg gaattgactg gattcctttt ttagggccca ttggtatggc tttttccccg
     5701 tatcccccca ggtgtctgca ggctcaaaga gcagcgagaa gcgttcagag gaaagcgatc
     5761 ccgtgccacc ttccccgtgc ccgggctgtc cccgcacgct gccggctcgg ggatgcgggg
     5821 ggagcgccgg accggagcgg agccccgggc ggctcgctgc tgccccctag cgggggaggg
     5881 acgtaattac atccctgggg gctttggggg ggggctgtcc ctgatatcta taacaagaaa
     5941 atatatatat aataagttat cacgtaagta gaacatgaaa taacaatata attatcgtat
     6001 gagttaaatc ttaaaagtca cgtaaaagat aatcatgcgt cattttgact cacgcggtcg
     6061 ttatagttca aaatcagtga cacttaccgc attgacaagc acgcctcacg ggagctccaa
     6121 gcggcgactg agatgtccta aatgcacagc gacggattcg cgctatttag aaagagagag
     6181 caatatttca agaatgcatg cgtcaatttt acgcagacta tctttctagg gttaatctag
     6241 ctgcatcagg atcatatcgt cgggtctttt ttccggctca gtcatcgccc aagctggcgc
     6301 tatctgggca tcggggagga agaagcccgt gccttttccc gcgaggttga agcggcatgg
     6361 aaagagtttg ccgaggatga ctgctgctgc attgacgttg agcgaaaacg cacgtttacc
     6421 atgatgattc gggaaggtgt ggccatgcac gcctttaacg gtgaactgtt cgttcaggcc
     6481 acctgggata ccagttcgtc gcggcttttc cggacacagt tccggatggt cagcccgaag
     6541 cgcatcagca acccgaacaa taccggcgac agccggaact gccgtgccgg tgtgcagatt
     6601 aatgacagcg gtgcggcgct gggatattac gtcagcgagg acgggtatcc tggctggatg
     6661 ccgcagaaat ggacatggat accccgtgag ttacccggcg ggcgcgcttg gcgtaatcat
     6721 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     6781 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     6841 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     6901 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     6961 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     7021 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     7081 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     7141 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     7201 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     7261 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     7321 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     7381 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     7441 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     7501 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     7561 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     7621 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     7681 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     7741 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     7801 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     7861 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     7921 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     7981 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     8041 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     8101 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     8161 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     8221 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     8281 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     8341 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     8401 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     8461 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     8521 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     8581 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     8641 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     8701 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     8761 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     8821 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt
     8881 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     8941 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     9001 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     9061 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     9121 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     9181 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     9241 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     9301 cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg
     9361 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatg
//
