LOCUS       sequence-297793-         418 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector expressed CasRx and sgRNA_Ptbp1 for AAV package.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Zhou H, Su J, Hu X, Zhou C, Li H, Chen Z, Xiao Q, Wang B, Wu W, Sun 
            Y, Zhou Y, Tang C, Liu F, Wang L, Feng C, Liu M, Li S, Zhang Y, Xu 
            H, Yao H, Shi L, Yang H
  TITLE     Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of 
            Neurological Disease in Mice.
  JOURNAL   Cell. 2020 Apr 30;181(3):590-603.e16. doi: 
            10.1016/j.cell.2020.03.024. Epub 2020 Apr 8.
  PUBMED    32272060
REFERENCE   2  (bases 1 to 418)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.03.024"; journalName: "Cell"; date: 
            "2020-04-30- 30"; volume: "181"; issue: "3"; pages: "590-603.e16"
COMMENT     Sequence Label: AAV-GFAP-NLS-CasRx-NLS-FLAG-U6-DR-SgRNA_Ptbp1-DR
FEATURES             Location/Qualifiers
     source          1..418
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..249
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1..21
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     172..191
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        251..286
                     /label=RfxCas13d DR36
                     /note="36-base direct repeat for an RfxCas13d pre-gRNA 
                     (Konermann et al., 2018)"
     misc_RNA        317..352
                     /label=RfxCas13d DR36
                     /note="36-base direct repeat for an RfxCas13d pre-gRNA 
                     (Konermann et al., 2018)"
     misc_RNA        383..418
                     /label=RfxCas13d DR36
                     /note="36-base direct repeat for an RfxCas13d pre-gRNA 
                     (Konermann et al., 2018)"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg caagtaaacc cctaccaact ggtcggggtt tgaaactgta gatgggctgt
      301 ccacgaagca ctggcgcaag taaaccccta ccaactggtc ggggtttgaa acgcttggag
      361 aagtcgatgc gcagcgtgca gccaagtaaa cccctaccaa ctggtcgggg tttgaaac
//
