LOCUS       sequence-297803-        3045 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast Expression plasmid for SARS-CoV-2 ORF10 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3045)
  TITLE     SARS-CoV-2 protein Yeast expression constructs, tagged, for protein 
            expression and/or purification
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4046615
FEATURES             Location/Qualifiers
     source          1..3045
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(36..827,828..896))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     659..678
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(897..999)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(1000..1031)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     gap             1033..1043
                     /estimated_length=11
     promoter        1098..1539
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    1098..1215
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     1510..1533
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     complement(1709..1727)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             2078..2881
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
ORIGIN
        1 gccatgttaa gggcggtttt ttcctgtttg gtcacttacc aatgcttaat cagtgaggca
       61 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
      121 ataactacga tgcgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
      181 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccgggag ggccgagcgc
      241 agaagtgatc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
      301 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
      361 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
      421 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
      481 gttgccagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
      541 tctcttactg tcatgccatc cgtgagatgc ttttctgtga ctggtgagta ctcaaccaag
      601 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
      661 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
      721 cgtaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
      781 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
      841 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatttta
      901 gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt
      961 tcattatggt gaaagttgga acctcttacg tgccgatcaa gtcaaaagcc tccggtcgga
     1021 ggcttttgac ttnnnnnnnn nnnggtcagg tatgatttaa atggtcagtt agagcggccg
     1081 cttgctatat tgaagtacgg attagaagcc gccgagcggg cgacagccct ccgacggaag
     1141 actctcctcc gtgcgtcctc gtcttcaccg gtcgcgttcc tgaaacgcag atgtgcctcg
     1201 cgccgcactg ctccgaacaa taaagattct acaatactag cttttatggt tatgaagagg
     1261 aaaaattggc agtaacctgg ccccacaaac cttcaaatta acgaatcaaa ttaacaacca
     1321 taggatgata atgcgattag ttttttagcc ttatttctgg ggtaattaat cagcgaagcg
     1381 atgatttttg atctattaac agatatataa atggaaaagc tgcataacca ctttaactaa
     1441 tactttcaac attttcagtt tgtattactt cttattcaaa tgtcataaaa gtatcaacaa
     1501 aaaattgtta atatacctct atactttaac gtcaaggaga aaaaactata atgggttaca
     1561 taaatgtctt tgcattccct ttcaccatct atagtcttct attatgcaga atgaactccc
     1621 gaaactacat tgcccaggtt gacgtagtta actttaattt gacttaaaca ggcccctttt
     1681 cctttgtcga tatcatgtaa ttagttatgt cacgcttaca ttcacgccct cctcccacat
     1741 ccgctctaac cgaaaaggaa ggagttagac aacctgaagt ctaggtccct atttattttt
     1801 tttaatagtt atgttagtat taagaacgtt atttatattt caaatttttc ttttttttct
     1861 gtacaaagcg gccgccgaac cactagtgca ctgcagtaca cggtttcctt gaaatttttt
     1921 tgattcggta atctccgaac agaaggaaga acgaaggaag gagcacagac ttagattggt
     1981 atatatacgc atatgtagtg ttgaagaaac atgaaattgc ccagtattct taacccaact
     2041 gcacagaaca aaaacgtgca ggaaacgaag ataaatcatg tcgaaagcta catataagga
     2101 acgtgctgct actcatccta gtcctgttgc tgccaagcta tttaatatca tgcacgaaaa
     2161 gcaaacaaac ttgtgtgctt cattggatgt tcgtaccacc aaggaattac tggagttagt
     2221 tgaagcatta ggtcccaaaa tttgtttact aaaaacacat gtggatatct tgactgattt
     2281 ttccatggag ggcacagtta agccgctaaa ggcattatcc gccaagtaca attttttact
     2341 cttcgaggac agaaaatttg ctgacattgg taatacagtc aaattgcagt actctgcggg
     2401 tgtatacaga atagcagaat gggcagacat tacgaatgca cacggtgtgg tgggcccngg
     2461 tattgttagc ggtttgaagc aggcggcaga agaagtaaca aaggaaccta gaggcctttt
     2521 gatgttagca gaattgtcat gcaagggctc cctatctact ggagaatata ctaagggtac
     2581 tgttgacatt gcgaagagcg acaaagattt tgttatcggc tttattgctc aaagagacat
     2641 gggtggaaga gatgaaggtt acgattggtt gattatgaca cccggtgtgg gtttagatga
     2701 caagggagat gcattgggtc aacagtatag aaccgtggat gatgtggttt ctacaggatc
     2761 tgacattatt attgttggaa gaggactatt tgcaaaggga agggatgcta aggtagaggg
     2821 tgaacgttac agaaaagcag gctgggaagc atatttgaga agatgcggcc agcaaaacta
     2881 aaaaactgta ttataagtaa atgcatgtat actaaactca caaattagag cttcaattta
     2941 attatatcag ttattacccg agtaacgatg catctgtgct tcattttgta gaacaaaaat
     3001 gcaacgcgag agcgctaatt tttcaaacaa agaatctgag ctgca
//
