LOCUS       sequence-297807-        2898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of codon-optimized SARS-CoV2 Nsp12 (RdRp) in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Meng W, Guo S, Cao S, Shuda M, Robinson-McCarthy LR, McCarthy KR, 
            Shuda Y, Paniz Mondolfi AE, Bryce C, Grimes Z, Sordillo EM, 
            Cordon-Cardo C, Li P, Zhang H, Perlman S, Guo H, Gao SJ, Chang Y, 
            Moore PS
  TITLE     Development and characterization of a new monoclonal antibody 
            against SARS-CoV-2 NSP12 (RdRp).
  JOURNAL   J Med Virol. 2023 Jan;95(1):e28246. doi: 10.1002/jmv.28246. Epub 
            2022 Nov 2.
  PUBMED    36271490
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Med 
            Virol."; date: "2023-01"; volume: "95(1):e28246. doi"; pages: " 
            10.1002/jmv.28246. Epub 2022 Nov 2"
COMMENT     Sequence Label: p3xFLAG SARS-CoV2 RdRp.co
FEATURES             Location/Qualifiers
     source          1..2898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2821..2886
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 gaattcaaca tgtccgcaga tgcgcaatct tttttgaaca gagtttgtgg tgtaagtgca
       61 gcccgtctta caccgtgcgg cacaggcact agtactgatg tcgtctatag ggcgttcgat
      121 atttataacg ataaagtggc gggcttcgca aaattcctta aaactaactg ctgtaggttc
      181 caggaaaaag acgaagatga caatcttata gacagctatt tcgtcgttaa acgacatact
      241 tttagcaact accaacatga ggaaacgatc tacaacttgc ttaaagattg tccggcggta
      301 gcgaagcacg acttcttcaa atttcggatc gatggagata tggttcctca catttccaga
      361 caacgcctga ctaaatacac tatggcagat cttgtttacg ccttgcgaca ttttgacgag
      421 ggtaactgcg atactcttaa ggagatcctc gttacctata attgctgcga tgatgattac
      481 tttaataaga aagactggta cgattttgta gagaaccctg atatcctccg ggtctatgcg
      541 aatctcgggg agcgagttag acaggcgctc cttaagacag tgcaattctg cgacgcaatg
      601 cggaatgccg gaatagtggg tgtacttacg cttgataatc aagacctcaa cggtaactgg
      661 tatgacttcg gagatttcat ccagaccacg cctggatcag gcgtccccgt cgtggattca
      721 tattactctc tgttgatgcc tattcttacc cttaccagag ctcttacagc tgaatctcat
      781 gtggatactg atctcactaa accatacatc aagtgggacc tcttgaaata tgattttact
      841 gaagaaaggc tgaagctgtt cgacaggtac tttaagtatt gggaccaaac atatcatccg
      901 aactgtgtga attgtcttga tgatcgatgc atactccatt gtgcgaattt taatgtactc
      961 ttctcaacag tcttcccacc tacaagtttt ggaccgctcg taagaaaaat ttttgtggac
     1021 ggagtccctt tcgtcgtctc tactggctat cactttcgag agttgggtgt cgttcacaac
     1081 caggacgtaa accttcacag tagtcgactt tcctttaaag aactgcttgt ctacgccgcc
     1141 gacccagcta tgcacgcagc cagtggaaac ctcttgctcg ataaacgaac tacctgcttt
     1201 agcgttgcgg ccttgacaaa caacgtcgca ttccagactg tgaagccagg taattttaac
     1261 aaagacttct atgactttgc agttagtaag ggattcttta aggaaggaag ttccgttgaa
     1321 ctcaaacatt tttttttcgc gcaagatggc aatgccgcta tctcagacta cgactattac
     1381 cgatataacc tgcccaccat gtgcgacata cgccaactgc tttttgtggt cgaagttgtg
     1441 gataagtact ttgactgcta tgatgggggt tgcattaatg caaaccaagt cattgttaat
     1501 aaccttgata aatccgctgg cttccccttt aataaatggg ggaaagcacg gttgtattat
     1561 gactctatgt cctacgagga tcaagatgcc ttgtttgcgt ataccaaaag gaacgttata
     1621 cccacaatta cgcagatgaa tctgaagtat gccataagtg cgaaaaatag ggcacgcacc
     1681 gtcgcaggag taagcatatg ttcaacaatg accaataggc aatttcatca aaaactgttg
     1741 aagtcaattg ccgccacgag aggcgctact gtcgtaattg gaacatcaaa attttatggg
     1801 ggctggcata acatgcttaa aacggtgtat tcagatgtcg agaatccgca cctcatgggc
     1861 tgggattacc cgaagtgcga ccgggcaatg ccgaacatgc tcaggatcat ggctagtttg
     1921 gtactggccc ggaaacatac cacatgctgt tcactcagtc atcgatttta tcgacttgcg
     1981 aacgagtgcg cccaagtcct gagcgaaatg gtaatgtgtg gaggctctct ctatgtaaaa
     2041 cccggtggta cctcctcagg agatgcaact accgcgtacg cgaactctgt atttaatatt
     2101 tgccaggcag taactgcgaa tgttaatgct ctgctgagca cagatgggaa taagatagcc
     2161 gataaatacg tgcgcaacct ccaacaccgc ttgtatgagt gtctgtatag aaatcgggac
     2221 gtagataccg actttgtgaa tgaattttac gcgtatctgc ggaagcattt ttctatgatg
     2281 attctgtccg acgatgcagt agtctgcttc aattcaactt atgcttccca aggcttggtc
     2341 gcctctataa aaaattttaa gtcagtcctg tattatcaaa ataatgtatt tatgtcagaa
     2401 gctaagtgtt ggacggagac cgatctgacg aaaggtcccc atgagttctg ctcccaacat
     2461 acaatgctgg tgaagcaagg tgatgattac gtttacctcc catacccaga tccgtcccgg
     2521 attcttgggg ctgggtgttt cgtggatgat attgtgaaaa ctgatggcac actcatgata
     2581 gagcggtttg tttccctggc tattgatgca taccctctga cgaagcaccc aaatcaagag
     2641 tacgcggacg tgtttcactt gtatctgcag tacattcgga agctgcacga cgaactgaca
     2701 ggacacatgc ttgatatgta cagtgtcatg ctcactaatg ataacacttc ccgctattgg
     2761 gagccggagt tttacgaagc gatgtataca ccccatactg tgcttcaggg gtcccgggcg
     2821 gactataagg accatgatgg ggattacaaa gatcatgaca tcgattataa agatgatgat
     2881 gacaaatgat aaggatcc
//
