LOCUS       sequence-297834-        2096 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial Expression plasmid for SARS-CoV-2 ORF7a protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2096)
  TITLE     SARS-CoV-2 protein E. coli expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4046901
FEATURES             Location/Qualifiers
     source          1..2096
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    20..44
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             59..81
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             452..472
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             473..490
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     rep_origin      611..1199
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1100..1119
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1305..1964
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1371..1390)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      2035..2069
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
       61 tgtttaactt taagaaggag actcttctat gaagatcata ctattcctcg ccttgatcac
      121 cctggcaacg tgtgaactgt atcactacca agagtgcgtt cgcggtacta ctgtcctgct
      181 taaagaacca tgcagctcag ggacctacga aggcaactct ccgtttcatc ctctggcgga
      241 caataaattc gctttaacat gcttttcgac tcagttcgct ttcgcttgtc ccgatggcgt
      301 aaaacacgtg taccagctgc gtgcgcgttc cgtttctccg aaactgttta ttcgacagga
      361 ggaagtgcag gaactgtatt ccccgatttt cctgatcgta gccgcaatcg ttttcatcac
      421 cctgtgcttt acccttaagc gcaaaactga ggagaacctg tactttcaaa gccaccatca
      481 tcaccatcat taaccggctt atcggtcagt ttcacctgat ttacgtaaaa acccgcttcg
      541 gcgggttttt gcttttggag gggcagaaag atgaatgact gtccacgacg ctatacccaa
      601 aagaaaaagc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
      661 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
      721 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
      781 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
      841 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
      901 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
      961 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     1021 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     1081 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     1141 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaat
     1201 tgatcgggca cgtaagaggt tccaactttc accataatga aataagatca ctaccgggcg
     1261 tattttttga gttatcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca
     1321 ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc
     1381 agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa
     1441 agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc
     1501 tgatgaatgc tcatccggaa tttcgtatgg caatgaaaga cggtgagctg gtgatatggg
     1561 atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct
     1621 ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt
     1681 gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgttt
     1741 cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact
     1801 tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc
     1861 cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta
     1921 atgaattaca acagtactgc gatgagtggc agggcggggc gtaatttgat atcgagctcg
     1981 cttggactcc tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga
     2041 acgctcggtt gccgccgggc gttttttatt ggtcatcgat ctcgatcccg cgaaat
//
