LOCUS       sequence-297841-        2996 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial Expression plasmid for SARS-CoV-2 N (nucleocapsid).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2996)
  TITLE     SARS-CoV-2 protein E. coli expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4046785
FEATURES             Location/Qualifiers
     source          1..2996
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    43..67
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             82..104
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1369..1389
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1390..1413
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     rep_origin      1534..2122
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2023..2042
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             2228..2887
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2294..2313)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      2958..2992
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 catcgatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa
       61 caattcccct ctagaaataa ttttgtttaa ctttaagaag gagactcttc tatgtcagat
      121 aatgggcctc aaaaccagcg caacgctcca cgtataacct tcggtggccc gtctgactcc
      181 acaggcagca accagaatgg tgagcgtagt ggagccaggt ctaagcagcg ccgtccccag
      241 ggtctgccga acaacacggc gagctggttt actgcgctta cccaacacgg taaagaagac
      301 ttaaaattcc cgcgtggcca gggcgtcccg atcaacacta actcttcccc ggatgatcag
      361 attggttatt accgcagagc aactcgtcgg attcgcggcg gcgacggtaa aatgaaagat
      421 ttgtctccgc gttggtactt ttactatctc ggcaccggtc cggaagctgg actgccctac
      481 ggggcaaaca aggacgggat catttgggtg gctaccgaag gtgccctgaa tactccaaaa
      541 gatcatatcg gcacccgtaa ccctgctaat aacgctgcga tcgttctgca actgcctcag
      601 ggtacaactc tgccaaaagg cttctatgca gaagggtcgc gtggtggcag ccaggcgagt
      661 agccgtagca gctcccgctc tcgcaactcg tcccgtaact ccactccggg ctcttctcgc
      721 ggcacgtccc cggctcgtat ggcaggtaac ggtggtgacg ctgctctggc gctgctactg
      781 ctggacaggc tgaaccagct ggagtcgaag atgagtggta aaggccaaca gcagcagggc
      841 cagaccgtta ccaaaaagag cgcagctgaa gcatctaaaa aaccgcgaca gaaacgtacc
      901 gcgactaaag cgtacaatgt aacacaggcc ttcggtcgtc gaggtccgga gcaaactcaa
      961 ggaaactttg gcgatcagga attaatccgc caggggaccg actacaaaca ctggccgcaa
     1021 attgcccagt tcgccccatc cgctagtgca ttcttcggta tgagccgcat cggcatggaa
     1081 gtaacgccaa gtggtacgtg gctgacctac accggtgcta tcaagcttga tgacaaggac
     1141 ccgaacttta aagaccaggt tattctgctt aacaaacata ttgatgccta taaaactttc
     1201 ccgccgaccg aacccaaaaa ggacaaaaag aaaaaagctg atgaaaccca ggctctaccg
     1261 cagcgtcaga aaaaacagca aactgtgact ctgctgccag ctgcggacct ggatgatttt
     1321 tccaagcagc tgcaacagtc tatgagctct gcggactcta cgcaggctga gaacctgtac
     1381 tttcaaagct ggagccatcc gcagtttgaa aaataaccgg cttatcggtc agtttcacct
     1441 gatttacgta aaaacccgct tcggcgggtt tttgcttttg gaggggcaga aagatgaatg
     1501 actgtccacg acgctatacc caaaagaaaa agcttgagat cctttttttc tgcgcgtaat
     1561 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     1621 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     1681 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     1741 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     1801 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     1861 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     1921 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     1981 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     2041 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     2101 aggggggcgg agcctatgga aattgatcgg gcacgtaaga ggttccaact ttcaccataa
     2161 tgaaataaga tcactaccgg gcgtattttt tgagttatcg agattttcag gagctaagga
     2221 agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg
     2281 taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca
     2341 gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc
     2401 ctttattcac attcttgccc gcctgatgaa tgctcatccg gaatttcgta tggcaatgaa
     2461 agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca
     2521 aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca
     2581 catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt
     2641 tattgagaat atgtttttcg tttcagccaa tccctgggtg agtttcacca gttttgattt
     2701 aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac
     2761 gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg tttgtgatgg
     2821 cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg
     2881 ggcgtaattt gatatcgagc tcgcttggac tcctgttgat agatccagta atgacctcag
     2941 aactccatct ggatttgttc agaacgctcg gttgccgccg ggcgtttttt attggt
//
