LOCUS       sequence-29787-e         645 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Vinkenborg JL, Nicolson TJ, Bellomo EA, Koay MS, Rutter GA, Merkx M
  TITLE     Genetically encoded FRET sensors to monitor intracellular Zn(2+) 
            homeostasis.
  JOURNAL   Nat Methods. 2009 Oct;6(10):737-40. doi: 10.1038/nmeth.1368.
  PUBMED    19718032
REFERENCE   2  (bases 1 to 645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2009-10"; volume: "6"; issue: 
            "10"; pages: "737-40"
COMMENT     Sequence Label: peZinCh-NB
FEATURES             Location/Qualifiers
     source          1..645
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(132..383)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(174..195)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     523..542
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
       61 gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tctagagtcg
      121 cggccgcttt acttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac
      181 tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc ggactggtag
      241 ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg
      301 tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc
      361 ttgatgccgt tcttctgctt gtcggccatg atatagacgt tgtggctgtt gtagttgtac
      421 tcaagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg
      481 cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg
      541 tccttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag
      601 tcgtgctgct tcatgtggtc ggggtagcgg gcgaagcaca tcagg
//
