LOCUS       sequence-297873-       10220 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses PTPN11 T73I.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10220)
  AUTHORS   Cremer A, Ellegast JM, Alexe G, Frank ES, Ross L, Chu SH, Pikman Y, 
            Robichaud A, Goodale A, Haupl B, Mohr S, Rao AV, Walker AR, Blachly 
            JS, Piccioni F, Armstrong SA, Byrd JC, Oellerich T, Stegmaier K
  TITLE     Resistance Mechanisms to SYK Inhibition in Acute Myeloid Leukemia.
  JOURNAL   Cancer Discov. 2020 Feb;10(2):214-231. doi: 
            10.1158/2159-8290.CD-19-0209. Epub 2019 Nov 26.
  PUBMED    31771968
REFERENCE   2  (bases 1 to 10220)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-19-0209"; journalName: "Cancer Discov"; date: 
            "2020-02"; volume: "10"; issue: "2"; pages: "214-231"
COMMENT     Sequence Label: pLX307/PTPN11 T73I
FEATURES             Location/Qualifiers
     source          1..10220
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2823..3033
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        2845..3041
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2892..3027
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3073..3675
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(3073..3092)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3569..3589
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3718..3835
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3973..5151
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4204..5142
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5099..5119
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    5171..5195
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7006..7030)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7038..7079
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    7130..7718
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7183..7203)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7601..7612)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7790..8023
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8101..8222
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8138..8157)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8192..8211
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8262..8397
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8417..8436)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8418..8436)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8446..8463)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8446..8462)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8455..8477)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8604..9059
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8691..8710)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8901..8922
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9085..9189
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9190..9258,9259..10050)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9408..9427)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccatgc attgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     2821 cagcaggcag aagtatgcaa agcatgcgtc tcaattagtc agcaaccata gtcccgcccc
     2881 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     2941 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     3001 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctttct agaggtacca
     3061 ccggagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     3121 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     3181 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     3241 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgccgt ggcggtctgg
     3301 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     3361 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     3421 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     3481 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     3541 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     3601 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     3661 cccggtgcca gttaacgaat tctagatctt gagacaaatg gcagtattca tccacaattt
     3721 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     3781 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
     3841 ttattacagg gacagcagag atccactttg gcgccggctc gaggcctgca ggtgcaaaga
     3901 tggataaagt tttaaacaga gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa
     3961 ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     4021 gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta
     4081 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     4141 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     4201 caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc
     4261 gtgccttgaa ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt
     4321 ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga
     4381 gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc
     4441 tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg
     4501 ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg
     4561 gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg
     4621 cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct
     4681 gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc
     4741 cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc
     4801 tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa
     4861 agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc
     4921 aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg
     4981 ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg
     5041 cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc
     5101 aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg aggctagcat
     5161 cgattgatca acaagtttgt acaaaaaagc aggctccgcg gccgccccct tcaccatggc
     5221 atcgcggaga tggtttcacc caaatatcac tggtgtggag gcagaaaacc tactgttgac
     5281 aagaggagtt gatggcagtt ttttggcaag gcctagtaaa agtaaccctg gagacttcac
     5341 actttccgtt agaagaaatg gagctgtcac ccacatcaag attcagaaca ctggtgatta
     5401 ctatgacctg tatggagggg agaaatttgc cattttggct gagttggtcc agtattacat
     5461 ggaacatcac gggcaattaa aagagaagaa tggagatgtc attgagctta aatatcctct
     5521 gaactgtgca gatcctacct ctgaaaggtg gtttcatgga catctctctg ggaaagaagc
     5581 agagaaatta ttaactgaaa aaggaaaaca tggtagtttt cttgtacgag agagccagag
     5641 ccaccctgga gattttgttc tttctgtgcg cactggtgat gacaaagggg agagcaatga
     5701 cggcaagtct aaagtgaccc atgttatgat tcgctgtcag gaactgaaat acgacgttgg
     5761 tggaggagaa cggtttgatt ctttgacaga tcttgtggaa cattataaga agaatcctat
     5821 ggtggaaaca ttgggtacag tactacaact caagcagccc cttaacacga ctcgtataaa
     5881 tgctgctgaa atagaaagca gagttcgaga actaagcaaa ttagctgaga ccacagataa
     5941 agtcaaacaa ggcttttggg aagaatttga gacactacaa caacaggagt gcaaacttct
     6001 ctacagccga aaagagggtc aaaggcaaga aaacaaaaac aaaaatagat ataaaaacat
     6061 cctgcccttt gatcatacca gggttgtcct acacgatggt gatcccaatg agcctgtttc
     6121 agattacatc aatgcaaata tcatcatgcc tgaatttgaa accaagtgca acaattcaaa
     6181 gcccaaaaag agttacattg ccacacaagg ctgcctgcaa aacacggtga atgacttttg
     6241 gcggatggtg ttccaagaaa actcccgagt gattgtcatg acaacgaaag aagtggagag
     6301 aggaaagagt aaatgtgtca aatactggcc tgatgagtat gctctaaaag aatatggcgt
     6361 catgcgtgtt aggaacgtca aagaaagcgc cgctcatgac tatacgctaa gagaacttaa
     6421 actttcaaag gttggacaag ggaatacgga gagaacggtc tggcaatacc actttcggac
     6481 ctggccggac cacggcgtgc ccagcgaccc tgggggcgtg ctggacttcc tggaggaggt
     6541 gcaccataag caggagagca tcatggatgc agggccggtc gtggtgcact gcagtgctgg
     6601 aattggccgg acagggacgt tcattgtgat tgatattctt attgacatca tcagagagaa
     6661 aggtgttgac tgcgatattg acgttcccaa aaccatccag atggtgcggt ctcagaggtc
     6721 agggatggtc cagacagaag cacagtaccg atttatctat atggcggtcc agcattatat
     6781 tgaaacacta cagcgcagga ttgaagaaga gcagaaaagc aagaggaaag ggcacgaata
     6841 tacaaatatt aagtattctc tagcggacca gactagtgga gatcagagcc ctctcccgcc
     6901 ttgtactcca acgccaccct gtgcagaaat gagagaagac agtgctagag tctatgaaaa
     6961 cgtgggcctg atgcaacagc agaaaagtta agggtgggcg cgccgaccca gctttcttgt
     7021 acaaagtggt tgatatcggt aagcctatcc ctaaccctct cctcggtctc gattctacgt
     7081 agtaatgaac tagtaccggt taagtcgaca atcaacgcgt taagtcgaca atcaacctct
     7141 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     7201 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     7261 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     7321 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     7381 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     7441 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     7501 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     7561 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     7621 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     7681 tcagacgagt cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact
     7741 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     7801 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
     7861 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     7921 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     7981 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtacgta tagtagttca
     8041 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     8101 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     8161 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     8221 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc
     8281 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     8341 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     8401 ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt
     8461 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     8521 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     8581 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     8641 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     8701 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     8761 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     8821 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     8881 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     8941 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     9001 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta
     9061 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     9121 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     9181 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     9241 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     9301 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     9361 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     9421 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     9481 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     9541 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     9601 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     9661 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     9721 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     9781 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     9841 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     9901 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     9961 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
    10021 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
    10081 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
    10141 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
    10201 gaaaagatca aaggatcttc
//
