LOCUS       sequence-297902-        5785 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Triggers HilA overexpression from Salmonella upon induction with 
            arabinose without any synthetic protein expressed.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5785)
  AUTHORS   Chabloz A, Schaefer JV, Kozieradzki I, Cronin SJF, Strebinger D, 
            Macaluso F, Wald J, Rabbitts TH, Pluckthun A, Marlovits TC, 
            Penninger JM
  TITLE     Salmonella-based platform for efficient delivery of functional 
            binding proteins to the cytosol.
  JOURNAL   Commun Biol. 2020 Jul 3;3(1):342. doi: 10.1038/s42003-020-1072-4.
  PUBMED    32620833
REFERENCE   2  (bases 1 to 5785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2020-07-3"; volume: "3(1):342. doi"; pages: " 
            10.1038/s42003-020-1072-4"
COMMENT     Sequence Label: pCASP-HilA empty vector
FEATURES             Location/Qualifiers
     source          1..5785
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      23..109
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(217..1095)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1122..1406
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1329..1348
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     misc_feature    3645..3703
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(3736..3753)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      3906..3977
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(4134..4722)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4214..4233)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(4810..4904)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(4925..5584)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     5499..5518
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(5585..5687)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     5753..5771
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 taaagcggcc gctgattggc atcaaataaa acgaaaggct cagtcgaaag actgggactt
       61 tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgcccta
      121 gaccgatgca taatgtgcct gtcaaatgga cgaagcaggg attctgcaaa ccctatgcta
      181 ctccgtcaag ccgtcaattg tctgattcgt taccaattat gacaacttga cggctacatc
      241 attcactttt tcttcacaac cggcacggaa ctcgctcggg ctggccccgg tgcatttttt
      301 aaatacccgc gagaaataga gttgatcgtc aaaaccaaca ttgcgaccga cggtggcgat
      361 aggcatccgg gtggtgctca aaagcagctt cgcctggctg atacgttggt cctcgcgcca
      421 gcttaagacg ctaatcccta actgctggcg gaaaagatgt gacagacgcg acggcgacaa
      481 gcaaacatgc tgtgcgacgc tggcgatatc aaaattgctg tctgccaggt gatcgctgat
      541 gtactgacaa gcctcgcgta cccgattatc catcggtgga tggagcgact cgttaatcgc
      601 ttccatgcgc cgcagtaaca attgctcaag cagatttatc gccagcagct ccgaatagcg
      661 cccttcccct tgcccggcgt taatgatttg cccaaacagg tcgctgaaat gcggctggtg
      721 cgcttcatcc gggcgaaaga accccgtatt ggcaaatatt gacggccagt taagccattc
      781 atgccagtag gcgcgcggac gaaagtaaac ccactggtga taccattcgc gagcctccgg
      841 atgacgaccg tagtgatgaa tctctcctgg cgggaacagc aaaatatcac ccggtcggca
      901 aacaaattct cgtccctgat ttttcaccac cccctgaccg cgaatggtga gattgagaat
      961 ataacctttc attcccagcg gtcggtcgat aaaaaaatcg agataaccgt tggcctcaat
     1021 cggcgttaaa cccgccacca gatgggcatt aaacgagtat cccggcagca ggggatcatt
     1081 ttgcgcttca gccatacttt tcatactccc gccattcaga gaagaaacca attgtccata
     1141 ttgcatcaga cattgccgtc actgcgtctt ttactggctc ttctcgctaa ccaaaccggt
     1201 aaccccgctt attaaaagca ttctgtaaca aagcgggacc aaagccatga caaaaacgcg
     1261 taacaaaagt gtctataatc acggcagaaa agtccacatt gattatttgc acggcgtcac
     1321 actttgctat gccatagcat ttttatccat aagattagcg gatcctacct gacgcttttt
     1381 atcgcaactc tctactgttt ctccataccc gtttttttgg gctagcgaat tcgagctcgg
     1441 tacccgagcc cgtagaatat gacattaagc tcataataaa agctcaacct gaccgttagt
     1501 actaacagca gaattactga aacagtagat tctatcctaa cgacttgtat tagttattat
     1561 aacttttcac cctgtaagag aatacactat tatcatgcca cattttaatc ctgttcctgt
     1621 atcgaataaa aaattcgtct ttgatgattt catactcaac atggacggct ccctgctacg
     1681 ctcagaaaag aaagtcaata ttccgccaaa agaatatgcc gttctggtca tcctgctcga
     1741 agccgccggc gagattgtga gtaaaaacac cttactggac caggtatggg gcgacgcgga
     1801 agttaacgaa gaatctctta cccgctgtat ttatgcctta cgacgtattc tgtcggaaga
     1861 taaagagcat cgttacattg aaacactgta cggacagggc tatcggttta atcgtccggt
     1921 cgtagtggtg tctccgccag cgccgcaacc tacgactcat acattggcga tacttccttt
     1981 tcagatgcag gatcaggttc aatccgagag tctgcattac tctatcgtga agggattatc
     2041 gcagtatgcg ccctttggcc tgagcgtgct gccggtgacc attacgaaga actgccgcag
     2101 tgttaaggat attcttgagc tcatggatca attacgcccc gattattata tctccgggca
     2161 gatgataccc gatggtaatg ataatattgt acagattgag atagttcggg ttaaaggtta
     2221 tcacctgctg caccaggaaa gcattaagtt gatagaacac caacccgctt ctctcttgca
     2281 aaacaaaatt gcgaatcttt tgctcagatg tattcccgga cttcgctggg acacaaagca
     2341 gattagcgag ctaaattcga ttgacagtac tatggtttac ttacgcggta agcatgagtt
     2401 aaatcaatac accccctata gcttacagca agcgcttaaa ttgctgactc aatgcgttaa
     2461 catgtcgcca aacagcattg cgccttactg tgcgctggca gaatgctacc tcagcatggc
     2521 gcaaatgggg atttttgata aacaaaacgc tatgatcaaa gctaaagaac atgcgattaa
     2581 ggcgacagag ctggaccaca ataatccaca agctttagga ttactggggc taattaatac
     2641 gattcactca gaatacatcg tcgggagttt gctattcaaa caagctaact tactttcgcc
     2701 catttctgca gatattaaat attattatgg ctggaatctt ttcatggctg gtcagttgga
     2761 ggaggcctta caaacgatta acgagtgttt aaaattggac ccaacgcgcg cagccgcagg
     2821 gatcactaag ctgtggatta cctattatca taccggtatt gatgatgcta tacgtttagg
     2881 cgatgaatta cgctcacaac acctgcagga taatccaata ttattaagta tgcaggttat
     2941 gtttctttcg cttaaaggta aacatgaact ggcacgaaaa ttaactaaag aaatatccac
     3001 gcaggaaata acaggactta ttgctgttaa tcttctttac gctgaatatt gtcagaatag
     3061 tgagcgtgcc ttaccgacga taagagaatt tctggaaagt gaacagcgta tagataataa
     3121 tccgggatta ttaccgttag tgctggttgc ccacggcgaa gctattgccg agaaaatgtg
     3181 gaataaattt aaaaacgaag acaatatttg gttcaaaaga tggaaacagg atccccgctt
     3241 gattaaatta cggaatctga gagaggagat atgcattatt tttttatcat cgtaatctgg
     3301 ttgcttagca taaatacggc atgggctgat tgctggcttc aggctgaaaa aatgttcaat
     3361 attgaatccg aactacttta cgctatcgcc cagcaggaat cggcgatgaa acctggcgcc
     3421 attggtcata accgagatgg ttcaaccgat cttggcctga tgcaaattaa cagcttccat
     3481 atgaaaaggc tgaaaaaaat ggggattagt gaaaaacagt tgttacagga cccctgcatt
     3541 tctgtcattg tgggcgcctc cattttatca gatatgatga aaatctacgg ttatagctgg
     3601 gaggccgttg gcgcttataa tgccgggacg tcgccgaaac gatcgaattc gagctcggta
     3661 cccgggggga tcctctagag tcgacctgca ggcatgcaag cttggctgtt ttggcggatg
     3721 agagaagatt ttcagcctga tacagattaa atcagaacgc agaagcggtc tgataaaaca
     3781 gaatttgcct ggcggcagta gcgcggtggt cccacctgac cccatgccga actcagaagt
     3841 gaaacgccgt agcgccgatg gtagtgtggg gtctccccat gcgagagtag ggaactgcca
     3901 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     3961 tgtcggtgaa cgctctcctg agtctagggc gttcggctgc ggcgagcggt atcagctcac
     4021 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     4081 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     4141 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     4201 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     4261 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     4321 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     4381 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     4441 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     4501 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     4561 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     4621 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     4681 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     4741 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgact
     4801 agtgcttgga ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc
     4861 cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat
     4921 caaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc
     4981 cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct
     5041 tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat
     5101 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca
     5161 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt
     5221 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa
     5281 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca
     5341 gctcaccgtc tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa
     5401 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg
     5461 taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa
     5521 aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg atttttttct
     5581 ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg
     5641 atcttatttc attatggtga aagttggaac ctcttacgtg ccgatcaacg tctcattttc
     5701 gccagatatc gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     5761 tatcacgagg ccctttcgtc ttcac
//
