LOCUS       sequence-297921-        4488 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for SARS-CoV-2 nsp7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4488)
  TITLE     SARS-CoV-2 protein Mammalian expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 4488)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4134036
FEATURES             Location/Qualifiers
     source          1..4488
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             531..551
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             552..578
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    631..752
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(642..661)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     696..715
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      867..913
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      943..1079
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    1124..1195
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     rep_origin      complement(1421..2009)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1501..1520)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(2755..2823,2824..3615)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2973..2992)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      3628..3764
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    3916..3987
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(3992..4038)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    4056..4104
                     /note="synthetic polyadenylation signal"
     enhancer        4185..4488
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ctgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatcagatc tttgtcgatc
      241 ctaccatcca ctcgacacac ccgccagcgg ccgccaccat gagcaaaatg tctgacgtca
      301 agtgtacatc cgtagttctg ttatcagtgc ttcaacagct gcgggtggag agttcgtcaa
      361 agctctgggc ccagtgcgtg cagctgcaca atgatatatt gctcgcgaaa gatactacgg
      421 aagctttcga gaagatggtc agcttgctat ctgttctact gagcatgcaa ggagcagtag
      481 acatcaacaa actttgcgaa gagatgttag acaaccgagc caccctgcaa gaaaaccttt
      541 actttcagtc gtacccctat gacgtaccag attatgcgta agctagcttg actgactgag
      601 atacagcgta ccttcagctc acagacatga taagatacat tgatgagttt ggacaaacca
      661 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      721 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
      781 ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
      841 gtattggccc atctctatcg gtatcgtagc ataacccctt ggggcctcta aacgggtctt
      901 gaggggtttt ttgtgcccct cgggccggat tgctatctac cggcattggc gcagaaaaaa
      961 atgcctgatg cgacgctgcg cgtcttatac tcccacatat gccagattca gcaacggata
     1021 cggcttcccc aacttgccca cttccatacg tgtcctcctt accagaaatt tatccttaag
     1081 gtcgtcagct atcctgcagg cgatctctcg atttcgatca agacattcct ttaatggtct
     1141 tttctggaca ccactagggg tcagaagtag ttcatcaaac tttcttccct ccctaatctc
     1201 attggttacc ttgggctatc gaaacttaat taaccagtca agtcagctac ttggcgagat
     1261 cgacttgtct gggtttcgac tacgctcaga attgcgtcag tcaagttcga tctggtcctt
     1321 gctattgcac ccgttctccg attacgagtt tcatttaaat catgtgagca aaaggccagc
     1381 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     1441 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     1501 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     1561 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     1621 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     1681 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     1741 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     1801 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     1861 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     1921 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     1981 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     2041 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     2101 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     2161 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     2221 gtctatttcg ttcatccata gttgcattta aatttccgaa ctctccaagg ccctcgtcgg
     2281 aaaatcttca aacctttcgt ccgatccatc ttgcaggcta cctctcgaac gaactatcgc
     2341 aagtctcttg gccggccttg cgccttggct attgcttggc agcgcctatc gccaggtatt
     2401 actccaatcc cgaatatccg agatcgggat cacccgagag aagttcaacc tacatcctca
     2461 atcccgatct atccgagatc cgaggaatat cgaaatcggg gcgcgcctgg tgtaccgaga
     2521 acgatcctct cagtgcgagt ctcgacgatc catatcgttg cttggcagtc agccagtcgg
     2581 aatccagctt gggacccagg aagtccaatc gtcagatatt gtactcaagc ctggtcacgg
     2641 cagcgtaccg atctgtttaa acctagatat tgatagtctg atcggtcaac gtataatcga
     2701 gtcctagctt ttgcaaacat ctatcaagag acaggatcag caggaggctt tcgcatgagt
     2761 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     2821 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcgcgagtg
     2881 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     2941 cgctttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     3001 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     3061 tattcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     3121 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gattggagga
     3181 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     3241 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     3301 gcaatggcaa caaccttgcg taaactatta actggcgaac tacttactct agcttcccgg
     3361 caacagttga tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     3421 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     3481 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     3541 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     3601 attaagcatt ggtaaccgat tctaggtgca ttggcgcaga aaaaaatgcc tgatgcgacg
     3661 ctgcgcgtct tatactccca catatgccag attcagcaac ggatacggct tccccaactt
     3721 gcccacttcc atacgtgtcc tccttaccag aaatttatcc ttaagatccc gaatcgttta
     3781 aactcgactc tggctctatc gaatctccgt cgtttcgagc ttacgcgaac agccgtggcg
     3841 ctcatttgct cgtcgggcat cgaatctcgt cagctatcgt cagcttacct ttttggcagc
     3901 gatcgcggct cccgacatct tggaccatta gctccacagg tatcttcttc cctctagtgg
     3961 tcataacagc agcttcagct acctctcaat tcaaaaaacc cctcaagacc cgtttagagg
     4021 ccccaagggg ttatgctatc aatcgttgcg ttacacacac aaaaaaccaa cacacatcca
     4081 tcttcgatgg atagcgattt tattatctaa ctgctgatcg agtgtagcca gatctagtaa
     4141 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     4201 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     4261 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     4321 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     4381 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     4441 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatg
//
