LOCUS       sequence-297945-        2926 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses MobR-regulated 3WJdB RNA aptamer driven by T7 promoter 
            with the mobO sequence 5BP downstream from the promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Jung JK, Alam KK, Verosloff MS, Capdevila DA, Desmau M, Clauer PR, 
            Lee JW, Nguyen PQ, Pasten PA, Matiasek SJ, Gaillard JF, Giedroc DP, 
            Collins JJ, Lucks JB
  TITLE     Cell-free biosensors for rapid detection of water contaminants.
  JOURNAL   Nat Biotechnol. 2020 Jul 6. pii: 10.1038/s41587-020-0571-7. doi: 
            10.1038/s41587-020-0571-7.
  PUBMED    32632301
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2020 Jul 6. pii: 10.1038/s41587-020-0571-7. doi: 
            10.1038/s41587-020-0571-7."
COMMENT     Sequence Label: pJBL718
FEATURES             Location/Qualifiers
     source          1..2926
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(79..98)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(758..1549,1550..1618))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1381..1400
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1619..1723)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1791..1809
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1847..1869)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1969..1988
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2182..2204
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2196..2213
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2197..2213
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(2239..2285)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(2462..2480)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2523..2539)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2523..2539)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2536..2558)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2547..2563
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2571..2577,2578..2595,2596..2601))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2616..2637
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2754..2771)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(2925..2926,1..587))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
       61 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
      121 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
      181 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
      241 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
      301 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
      361 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
      421 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
      481 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
      541 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
      601 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
      661 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
      721 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
      781 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
      841 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
      901 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
      961 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     1021 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     1081 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     1141 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     1201 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     1261 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     1321 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     1381 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     1441 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     1501 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     1561 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     1621 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     1681 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     1741 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     1801 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     1861 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     1921 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc
     1981 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     2041 aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
     2101 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
     2161 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattcg
     2221 agctcggtac ccggggatca aaaaacccct caagacccgt ttagaggccc caaggggtta
     2281 tgctacccac atacacatgg caagagccca cactctactc gacagatacg aatatctgga
     2341 cccgaccgtc tcttgccatg aatgatccga gcccacactc tactcgacag atacgaatat
     2401 ctggacccga ccgtctcgga tcatcagagt atgtgggtca gtccgcacac aaatagtacc
     2461 tcctatagtg agtcgtatta tctagagtcg acctgcaggc atgcaagctt ggcgtaatca
     2521 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     2581 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     2641 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     2701 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     2761 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     2821 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     2881 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttt
//
