LOCUS       sequence-29796-e         742 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 742)
  TITLE     Lawrence Cohen lab plasmids
REFERENCE   2  (bases 1 to 742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Zahra73-59
FEATURES             Location/Qualifiers
     source          1..742
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      88..97
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagcgcc accatggcca cgtctgtgca ttttaaccct
      121 gggttagatt ccaaagaagt taatggtaat tcggtgaaag aagaggcaga ggtgcagatc
      181 gatgatggaa aggaagaaac caaagaccca gacacaatgt accatcaggt ccggaaaaag
      241 ataactcctt ttgtgatgtc ctttggtttt cgtgtttttg gcctggtact gatcatcttg
      301 gacatcatta tggtcattgt ggacttgtct ctttctgaga agagtcgtga tgttggaggt
      361 gcaccggaga ctgtttctct ggtcatctct ttcttttttc tcatcgatgt attgctccgt
      421 gtttatgtgg agggattcaa ggtgtatttc agctcaaaac tgaatatagt agatgcttgt
      481 attgtggtca ttactctggt ggtcaccatg atctacgcat tctccgattt ctcaggagcc
      541 agtctgattc ccagggtggt gacattcctg aggtctctga ggatcctaat tctggtacgc
      601 atcttcagac tggcctcgca gaagagagag cttgaaaaag tcaccaggag aatggtttca
      661 gagaacaaaa ganatatcgc atggtttcta aggtgaagag ctgttcactg gggtggtccc
      721 aattctcgng nactcgacgg tg
//
