LOCUS       sequence-297977-       14339 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14339)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 14339)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHSU71.2-2.2
FEATURES             Location/Qualifiers
     source          1..14339
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     492..508
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     492..508
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(850..868)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      909..2251
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    1241..1288
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        2278..2382
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2383..2451,2452..3243)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2601..2620)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3414..4002
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3903..3922
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4339..4359
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4341..4359
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(8637..9440)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(9441..9661)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     terminator      complement(12560..12859)
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
ORIGIN
        1 gttaatagtt gatagttgat tgtatgcttt ttgtagcttg atattctatt taccaagaag
       61 aaacaagaag tgataaaaac aacaagagag cagtagtaag agtatttcga gtgtgaaaaa
      121 agtcgctact ggcactctat ttatatgtga taggcatgct atagctttac caaaaagtga
      181 accccatttc tatgctctcc tctgcctttt tttttttttt tttttcattc tctcaatctg
      241 aaattctctt atttctccaa cttataagtt ggagatgccc ggtgttccgg cagaggagat
      301 cagtctcgtg aagtggatgg tttcccgcct gcgggcaaaa cgtcataaca tttttatgag
      361 cgaaagccgt taatgaagac aaaatccctt aattaaaaca ttagaatggt gattagaaag
      421 gcaggattaa tcagttacac aggctgtaac cggagagacg gatcataagg caatttttag
      481 atattaatta atctagaact agtggatccc ccgggctgca ggaattcgat atcaagctta
      541 tcgataccgt catttaaatg cttgctacga tcttgggcat aagcctggtt caatacccgc
      601 gcttaaaggg taatttcgaa aacagcgagt gtccggtgtc ccaaatggtt acaaagtgca
      661 ccctgtacgc tagcacgacg gttgccacct catgcccatt cttggataga cagaaacagg
      721 attccctcgc caattttcca gcttctttgc gttgtatatt agggataaca gggtaatggc
      781 gcgccgaggt tttcaccgtc atcaccgaaa cgcgctcacc gtcatcaccg aaacgcgcga
      841 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
      901 cttagtatga tccaatatca aaggaaatga tagcattgaa ggatgagact aatccaattg
      961 aggagtggca gcatatagaa cagctaaagg gtagtgctga aggaagcata cgataccccg
     1021 catggaatgg gataatatca caggaggtac tagactacct ttcatcctac ataaatagac
     1081 gcatataagt acgcatttaa gcataaacac gcactatgcc gttcttctca tgtatatata
     1141 tatacaggca acacgcagat ataggtgcga cgtgaacagt gagctgtatg tgcgcagctc
     1201 gcgttgcatt ttcggaagcg ctcgttttcg gaaacgcttt gaagttccta ttccgaagtt
     1261 cctattctct agaaagtata ggaacttcag agcgcttttg aaaaccaaaa gcgctctgaa
     1321 gacgcacttt caaaaaacca aaaacgcacc ggactgtaac gagctactaa aatattgcga
     1381 ataccgcttc cacaaacatt gctcaaaagt atctctttgc tatatatctc tgtgctatat
     1441 ccctatataa cctacccatc cacctttcgc tccttgaact tgcatctaaa ctcgacctct
     1501 acatttttta tgtttatctc tagtattact ctttagacaa aaaaattgta gtaagaacta
     1561 ttcatagagt gaatcgaaaa caatacgaaa atgtaaacat ttcctatacg tagtatatag
     1621 agacaaaata gaagaaaccg ttcataattt tctgaccaat gaagaatcat caacgctatc
     1681 actttctgtt cacaaagtat gcgcaatcca catcggtata gaatataatc ggggatgcct
     1741 ttatcttgaa aaaatgcacc cgcagcttcg ctagtaatca gtaaacgcgg gaagtggagt
     1801 caggcttttt ttatggaaga gaaaatagac accaaagtag ccttcttcta accttaacgg
     1861 acctacagtg caaaaagtta tcaagagact gcattataga gcgcacaaag gagaaaaaaa
     1921 gtaatctaag atgctttgtt agaaaaatag cgctctcggg atgcattttt gtagaacaaa
     1981 aaagaagtat agattctttg ttggtaaaat agcgctctcg cgttgcattt ctgttctgta
     2041 aaaatgcagc tcagattctt tgtttgaaaa attagcgctc tcgcgttgca tttttgtttt
     2101 acaaaaatga agcacagatt cttcgttggt aaaatagcgc tttcgcgttg catttctgtt
     2161 ctgtaaaaat gcagctcaga ttctttgttt gaaaaattag cgctctcgcg ttgcattttt
     2221 gttctacaaa atgaagcaca gatgcttcgt tcaggtggca cttttcgggg aaatgtgcgc
     2281 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     2341 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     2401 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     2461 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     2521 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     2581 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     2641 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     2701 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     2761 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     2821 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     2881 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     2941 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     3001 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     3061 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     3121 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     3181 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     3241 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     3301 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     3361 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     3421 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     3481 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     3541 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     3601 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     3661 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     3721 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     3781 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     3841 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     3901 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     3961 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     4021 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     4081 cctgattctg tggataaccg tattaattaa tagggataac agggtaatgt gtagatcaag
     4141 ccctgaattg caaacgatta tgaagtgaca cgggtgttac ggcttgcgtc cagccagagt
     4201 tttttaggag catcaacgtt gcccctgggg tgtagtaact caccagtggc cgcccacgtg
     4261 atagtatgct ccataacctc caactagttt cgtctgtaag gcgggcaaaa tatatcttgt
     4321 ttgagtctcg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctccac
     4381 cgcggtggcg gccgcatagc gccgatcaaa gtatttgtta cgacaatatc gaataacgat
     4441 ggcttccccg ctatacttaa gaggatctcg caggctttac gtaaaagaga gatatacttt
     4501 aaaattgacg attccaatac ttcaattggt aaaaaatacg cccgtaacga tgaattggga
     4561 acaccatttg gtatcaccat agatttcgaa actataaaag accaaacggt gacgttaaga
     4621 gaaagaaatt ctatgaggca agtaagaggc actattactg atgtgatttc gaccattgac
     4681 aaaatgcttc acaaccctga tgaatcggac tgggataaat caacatttgg actgtcgcct
     4741 gttaagatat aactgaaaaa agaggggaat ttttagatac tgaaatgata ttttagaata
     4801 accagactat atataaggat aaattacaaa aaattaacta atagataaga tttaaatata
     4861 aaagatatgc aactagaaaa gtcttatcaa tctccttatg ctatggttct atggccttca
     4921 agagacaagg cttcttgtag agcttgatca accttcgata ttaggataga ctcatcttcc
     4981 agtccagcac tgattcttat gatgtgcttg gctatcccgt gagactccgc ccagtcgagt
     5041 tcataagcgt gtgccaactg ggaataaggt aagatcagcg taaagttggc gccgagactc
     5101 gggcccttgg gtaggtcaat agcgtcgtaa aaacgaatcg cactgttagg gtctctaaag
     5161 acgagactaa gtaaggcccc gtatccgcca ctgggtcgac ggtattgttc atatagggat
     5221 cgagtggcta ccattgaagg atggttcagc tgcaaaattg actcgcagga tttgagcaag
     5281 ttggcaattg ctagggcgtt ggtattcatt tttcgtatgc gggttggaaa gtcgagacag
     5341 ttgtgtagaa ggacctttgc gtccaaagga aacacgagat cctcatgtag ttcgttgagc
     5401 ttcgacttca gcaaatcata ccatggagac tttatgttca aaactgcgct tcctcccata
     5461 acattgcaag ccccgctgaa agccttcgtt aagctagtaa tgacgacgtc aacatagggt
     5521 aggacatcca cattgatgaa tgtgcctatt gtctcatcac ataccactgc gaagtgatat
     5581 tgatttgcaa gactacgaat gcgatggagg tctggcgtcg cgcatagagg attcgacgga
     5641 acttcgcaga agagactagc gatccgattg ccagatgcta gtgacgcttc cagctggtcc
     5701 aattcttctg atgtaccttg agaaaagaat gtgaaacgct catactcact cgccctaaca
     5761 cacctaggtg tcgatccgta gggccatccg aacacaactg cctcagaagc cttcgctgat
     5821 cctggagcaa gtatacttgc cacactgcga atggcacaca tgcctttggg gaaaaggtag
     5881 acatcatctg gattgactgc ctcttgccca ggttgatctg acgtgacaag tcttgcaata
     5941 attgttgtaa tgtctttttt ggcgtcggca tcggaatctt gccacggttc cataggaatc
     6001 gtttcgccat agctaccggt ttcaccggtt tgtagatcgg ggactaacga aaccgagctc
     6061 atgaattgga atctgtccag gcaaaattgt gcgtggcgac tggagattcc atcccccatc
     6121 tctccccaga actcattcgc agcgtctttg tcctcctggg aaaacagaac agcactgaag
     6181 cgggcccaag tttgattcgc ttgggatacg tgttgagtaa gagagaagga tacatcatga
     6241 accataatat taggcccctt tctcttgcga atgtacgcgc agcatctctt tgccgcgtcc
     6301 ttggaaggaa aaaccataca tcgcgtattc tccgggcttc ccaccctatc aagaacagct
     6361 tggttcaatt tctgcacagt tgggtgctga tagaacctcg gatagtagat ctgcaatgcg
     6421 tcgacaacag cctgatcatt cgccattttc ttaattcttt tatgtgcttt tactactttg
     6481 tttagttcaa aacaatagtc gttattctta ggtactatag cataagacaa gaaaagaaaa
     6541 ataagggaca aataacatta gcagaagtac ggtatatttt actgttactt atatactttc
     6601 aagaagatga gttaaatcgg tagccagtgt agaaaaataa taataagggt catcgatcct
     6661 tcgcatttta ttatccaatt aaagatacga atcacggcaa actatattca aagctcatag
     6721 ataatcgtcg taaggctgac actgcagaag aaaagtcata atttgaatac tagccggtat
     6781 gaaactgtga ttgattaacc tggggttacc taaagagaac ataagtaata ctcatgacag
     6841 aatcaaaaca caatacaaaa tttatccgaa cctcggcccg actgccggct cgccgggaaa
     6901 ggggacaacc gcttctatcc gtcgactaac ttcatcggcc caatggaggt atcatctcca
     6961 tctcccatat gcatatcact gtggtcgtgg cccgcatttc cttccatctt gtgattcatg
     7021 ctatccatct tttttgagta tccaattaac gaagacgtta ccagctgatt gaaggttctc
     7081 aaagtgactg tactccatgt tttcttatca tccatgtagt tatttttcaa actgcaaatt
     7141 caagaaaaag ccacgcgtgt gcaccttttt tttctccttc cagtgcatta tgcaatagac
     7201 agcacgagtc tttgaaaaag taacttataa aactgtatca atttttaaac ctaaatagat
     7261 tcataaacta ttcgttaata taaagtgttc taaactatga tgaaaaaata agcagaaaag
     7321 actaataatt cttagttaaa agcacttcat gtcttcttca acactaacat ccttcctccc
     7381 gtctctgtcc agtccaaaat cccaaattct tccgtgctca caatctgcaa aatcccatca
     7441 gactgtaact tttcaacttt cgctttgtag ccaccacttt cccaaatgtc attatgcact
     7501 gtagcaacaa gcaatccacc catgacagtc acacgggcaa gttctcctaa cacctcaggt
     7561 ccgacgtgac ccttggtcaa ggttcccaca caagttacaa catcataact cccatcagcc
     7621 ttatcaatct tctcgttcag attcgccctt tcaagttccc gatacacgcc ctttttgcgc
     7681 gccgctccta acatgcccac gctaagatcc acgccatcta gaacgaattt gttcgcaagg
     7741 gttgactggg ccaaacagtc gcccaccaga ccagtgccgc agccggcatc gaagatcttg
     7801 agtgcttccg aggagctagg tgagaggttg ttaacaatcg cctccacgca gcgccgggga
     7861 gaagcgtagc cattcgcatt atctgcatca tagacgtcgg cccattcatc atacagattt
     7921 tccgcttccg agctctcatt cagagcgtat gcgcgccgga gaaaatgatg aacacgtaaa
     7981 tcggaagtgt taggaggatg agacatgttg ttattttatt atggaataat tagttgcgtg
     8041 agtttggttt ttttttttta tttttgagtt aaacaaatat aattattgtt taagctgaaa
     8101 taaagatata tatgtatatc tgggaaaggc tgttggttat ctgtcttact tctgtatatt
     8161 taagaagtaa gtagaaatac tgattacaca tggaagagaa gttacacaca ttacaaagac
     8221 ttctctgccc ttatatacct tttttttgtg gtcttctata ctctgcccaa tgttgctatc
     8281 cccagccaag ccttacttaa tacacaaacg taccattgtc caaccagaag cgtctaagct
     8341 ttacaatcct gtttgggccc tttatccgcc tatccataat gttaagcagc ctaaaacttg
     8401 ctagtctagc cggcatcttt cccttgtata acttaaaata cccggagctt cagtgatatg
     8461 tagaacacag ccgcgccgga tgaaaggaag ctcggttttt tcctatcctg atgcggtatt
     8521 ttctccttac gcatctgtgc ggtatttcac accgcatagg gtaataactg atataattaa
     8581 attgaagctc taatttgtga gtttagtata catgcattta cttataatac agttttttag
     8641 ttttgctggc cgcatcttct caaatatgct tcccagcctg cttttctgta acgttcaccc
     8701 tctaccttag catcccttcc ctttgcaaat agtcctcttc caacaataat aatgtcagat
     8761 cctgtagaga ccacatcatc cacggttcta tactgttgac ccaatgcgtc tcccttgtca
     8821 tctaaaccca caccgggtgt cataatcaac caatcgtaac cttcatctct tccacccatg
     8881 tctctttgag caataaagcc gataacaaaa tctttgtcgc tcttcgcaat gtcaacagta
     8941 cccttagtat attctccagt agatagggag cccttgcatg acaattctgc taacatcaaa
     9001 aggcctctag gttcctttgt tacttcttct gccgcctgct tcaaaccgct aacaatacct
     9061 gggcccacca caccgtgtgc attcgtaatg tctgcccatt ctgctattct gtatacaccc
     9121 gcagagtact gcaatttgac tgtattacca atgtcagcaa attttctgtc ttcgaagagt
     9181 aaaaaattgt acttggcgga taatgccttt agcggcttaa ctgtgccctc catggaaaaa
     9241 tcagtcaaga tatccacatg tgtttttagt aaacaaattt tgggacctaa tgcttcaact
     9301 aactccagta attccttggt ggtacgaaca tccaatgaag cacacaagtt tgtttgcttt
     9361 tcgtgcatga tattaaatag cttggcagca acaggactag gatgagtagc agcacgttcc
     9421 ttatatgtag ctttcgacat gatttatctt cgtttcctgc aggtttttgt tctgtgcagt
     9481 tgggttaaga atactgggca atttcatgtt tcttcaacac tacatatgcg tatatatacc
     9541 aatctaagtc tgtgctcctt ccttcgttct tccttctgtt cggagattac cgaatcaaaa
     9601 aaatttcaag gaaaccgaaa tcaaaaaaaa gaataaaaaa aaaatgatga attgaattga
     9661 atgagttatg tccttcgcgc actgattcat ctttgaaact aaatttgtat attgtttgtc
     9721 agaaatagga cctgatatcc tccttttaaa cagttgatga gaaccttttt cgcaagttca
     9781 aggtgctcta atttttaaaa tttttacttt tcgcgacaca ataaagtctt cacgacgcta
     9841 aactattagt gcacataatg tagttacttg gacgctgttc aataatgtat aaaatttatt
     9901 tcctttgcat tacgtacatt atataaccaa atcttaaaaa tatagaaata tgatatgtgt
     9961 ataataatat aagcaaaatt tacgtatctt tgcttataat atagctttaa tgttctttag
    10021 gtattattta agagcgattt gttcagtatg aaactcgaag aacagcatct tgtttacatt
    10081 ttctttcaac agcgcctcga agcactttct tatccagctt ggctgagcta ttgagcggcc
    10141 agtcagcaag ctctaattgc gcaagtgtat aaactgcgcc tagaatgtac tccgagccaa
    10201 gatgggctaa gacgccatgg ataatcgctt gtgcagctga ttcgcttgcg tttgatggga
    10261 aatgatttga agagctcagc tccgtttttg tgagatggct atgccctttg tctgtggttg
    10321 ttgatggcag tgatttcaac acagcaacgg gcatctctcc gtagtcgaca tcaggtacac
    10381 cgatgactgt tgcctggacg ccgaaagaat cctttatata cccttcaatt accggaggga
    10441 aaagtccaat ccctctgcac ttgattgtat cttttatcct tccgactacg tataccaccc
    10501 cgtcatcatc catcaccgcc caatcaccgg ttcgaaacca gcgcccctgg gcgtcttcat
    10561 agaaactgga acttgacaca ttgccgagat agctatgaat cagcacagga gaagacacgt
    10621 gaagttctcc tcggaccccc ctaggcacag gcgttcgaga tgattccttg gccgaacaaa
    10681 tctttgcttt ggttcccggc aacgcgcgac caatgcccgt atcgccgctt tccaacatcg
    10741 gaagcgaggc caaggtgtta tctgcaactg agaagccaaa cattggcgaa ccttctgaag
    10801 cgccccaatg aggaacaaac cgacgcggct tcaaggcttg tcgagcgcta cgaatgagct
    10861 ccttgtcgga cacatcaccc gctggggaaa tgaacaagat ctcgtgttcg agcgagtgat
    10921 tctgctgcac aaattcggtt actagagcgc gaagctgcgt cggcagtatc atgagatgcg
    10981 tccctcgctc cgccttcaag ccctctatcg atgctgctgg atcaaaaaaa ggggatgtaa
    11041 agatgacgct gccgcccgcg atccacgaac ttagacaacc caaatagcac aatgggcgga
    11101 agagactagt ggtcaccaga aacctcgccg cctgcgacca gctgctatat gcgaatgcgt
    11161 ggtgcccctc aatttcggcg tggatattgg caaacgtaag gcaacaaccc tttggcgtac
    11221 tggttgtgcc ggatgtgaac atgatcagag ctggattcgt aggtggcgac acggatggtg
    11281 gaggagacga tgaggaattt gccgggtagg tcaagtgtga tagtgatgac cacctttcgc
    11341 tgcaggctcc ggcgtttcca ccgcaaatga gccaaatgac tgcgttcttc tctcccacgt
    11401 cgttcaccac ccaagacttc gtgtctatcc cgtcaccgag gaccacgacc tttggtttca
    11461 acagtttgta aacctcattc agttctttcg ttcgcgccaa cagggaaggc tctactggag
    11521 caaaaacggc tccaatcctc caacatgtca acagaagaag ggtccactcg actgcgtaat
    11581 tgttgccaat gaaagaagcc actacatcac tctcgcggac acccttttca taaagaatgg
    11641 ttgccaagtt ctcaacggca gtgacaagtt gctcgtagga gcatcggaac ccaacatcgg
    11701 cggatgacga accgggagat gatttagagg gcgcgaaaag attagatagg tgtcccgggg
    11761 gttgatgacg agatacaaga gccaatcttg aagcctgatg gcgggcacca agagagagca
    11821 attgccaaaa gttggcaggg gaaggcgagg ctgggccacc gtccgctgtt gaaagaagct
    11881 gtgaatgggt cattttgtat tgtattttga ggatagagtt gacagttgat agtatgctta
    11941 atatagcttg atatacttat atactaagta gaaacaagag aattgtaacc tgaaaaaggg
    12001 agaagaccac taacaagaca gattttgaga taaacagggt ggcaatggcc actctattta
    12061 tacgcaccca gtatgcttga tctgaccgac tgtgtgtgag tgtgtgacat aagggaacta
    12121 actccatttg cgctgtgctg tacctttttt ttttcttcca ttaaacctgc gatgctctta
    12181 atttccccaa cttgcaagtt ggagatggcc gttgttccgg tggtaggtca ggccggtgga
    12241 aaatgggtag tttcgtgtct atgggttaaa aaaaacgtca tttttgggcg catgccgttg
    12301 atggagacaa aaatccctta attaagcatc agcataggtg attagaaaga aaggtttgac
    12361 cagataaagg ctgtgtcact ggagaactgg atcgtgaggg ttcaataatt gaaattatag
    12421 ggtggacgtc aagacgaaaa gtgaaaaatt acatccgtat agaattatat aacttgatga
    12481 gatgagatga gtaaatgaca gaagaattac cgtttcatca ttgaacttcg atcatttcaa
    12541 tgctggcatg cgaaggaaaa tgagaaatat cgagggagac gattcagagg agcaggacaa
    12601 actataaccg actgtttgtt ggaggatgcc gtacataacg aacactgctg aagctaccat
    12661 gtctacagtt tagaggaatg ggtacaactc acaggcgagg gatggtgttc actcgtgcta
    12721 gcaaacgcgg tgggagcaaa aagtagaata ttatctttta ttcgtgaaac ttcgaacact
    12781 gtcatctaaa gatgctatat actaatatag gcatacttga taatgaaaac tataaatcgt
    12841 aaagacataa gagatccgct tactcgccgt cacgcttgcc ctccacaaga gccaagtcta
    12901 cctctgtctc aactgcgtcg taggcctcgt tcaacagtct cacaagatcc tcaaactcgt
    12961 cgtctgatgc attataagga ggcgcaagta tgacatggtc ccccttgaaa ccgtccgctg
    13021 tgcccgcccc gggataaatt gacaggtggc ggcggaaagc ctcatcaacc agcttccacg
    13081 taaagcgaag ggctggcgga aagggctcct tagtggcccg attctggaca aattcgattc
    13141 cccagaacag gcccctcccg cggatattcc cgacatactt gcgctggcct attgttgcat
    13201 tcagggccgc ctcgaaccat cgccccttgg ccgcacagcg agcgacaagg ccatcccggc
    13261 gaagcacttt ctgcacggct agggcggcgg cacaacccac ggcatgggcc tgataggtat
    13321 gcgaatgaac aaagtaggag ctgccactct gcagcccgcg gatcaccttt tcgtgcgcca
    13381 acacggctcc catgggggcg tatcctcctc ccagcccctt acccatggtg agcaggtccg
    13441 gatacacgcc cgggccctcc tgctcaaacg cgaaatacgt gcccgtccgg ccactaccgc
    13501 acatgacttc gtcgaggatg agcaggatgc cgtacctgtc acacagccgg cggacacctt
    13561 ggtagtagcc ctttggggcc gtgatgcatc ccgctgtggc gcctcctaca ggctccgcca
    13621 cgaaggcgat gaccgtttca ggcccgacag actggaactg ttcatcaagc tcctggacaa
    13681 gcctcgcgct gtactcttcc tcggattctc cctcgagttg cccgcggtag gcataggttg
    13741 cgctcacgta gctcacgtta ttcaagacca acgcatccag gaaaggcgcc cgtcttgaca
    13801 catggctcga cacactcagc gcgccgactg tgttgccatg gtatgcgcgc tgccgggaca
    13861 cgagtcgggt tcgctgcggt tggccgatct caacgtagta ctgtcttgcg agcttgagag
    13921 cggaatccat agcctcgctg ccggatgaga caaagaacgc tcgagagagc ccatgtgggt
    13981 ttccctgaag aatttcgttc gctaaggctt cggcgacggg ggttgtgaat gccacggtgt
    14041 gcgtgtagga caccttggac atctgctcct gaacagcagc cagcacctca gggttgccat
    14101 gcccgatgat ggcgacggcc gcaccagtac acccatcaag gacacgccga ccagtaccga
    14161 aagtcacata ggaaccctca gcagatacca ccgtcggagg gatggagatg agcgagttgt
    14221 gcagaacgtg ggatgcctca agatctagct ggccattggt gccgttaatc tcctttttct
    14281 ctgatgggct ggttcccgtc tgggtttgag tatcggaaac cattgtgtat tacgatata
//
