LOCUS       sequence-29798-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  TITLE     Lawrence Cohen lab plasmids
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Zahra76-77
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      88..97
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagcgcc accatggcca cgtctgtgca ttttaaccct
      121 gggttagatt ccaaagaagt taatggtaat tcggtgaaag aagaggcaga ggtgcagatc
      181 gatgatggaa aggaagaaac caaagaccca gacacaatgt accatcaggt ccggaaaaag
      241 ataactcctt ttgtgatgtc ctttggtttt cgtgtttttg gcctggtact gatcatcttg
      301 gacatcatta tggtcattgt ggacttgtct ctttctgaga agagtcgtga tgttggaggt
      361 gcaccggaga ctgtttctct ggtcatctct ttcttttttc tcatcgatgt attgctccgt
      421 gtttatgtgg agggattcaa ggtgtatttc agctcaaaac tgaatatagt agatgcttgt
      481 attgtggtca ttactctggt ggtcaccatg atctacgcat tctccgattt ctcaggagcc
      541 agtctgattc ccagggtggt gagattcctg aggtctctga ggatcctaat tctggtacgc
      601 atcttcagac tggcctcgca gaagagagag cttgnnaagt caccaggaga atggtttcag
      661 agaacaaaag aagatatcgc atggtttctn anggntgaag agctgttcac tggggtggtc
      721 ccaattctcg tgnactcgac ggtgacgtca acggccacaa attttctgtc tcnggagaag
      781 gagagggtga cgcaacctat gggaaactca ctctcaaatt catctgc
//
