LOCUS       sequence-297986-       18207 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18207)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 18207)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHSU75.2-2.2
FEATURES             Location/Qualifiers
     source          1..18207
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     524..540
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     524..540
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(882..900)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      941..2283
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    1273..1320
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        2310..2414
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2415..2483,2484..3275)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2633..2652)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3446..4034
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3935..3954
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4371..4391
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4373..4391
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(12335..13138)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(13139..13359)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     terminator      complement(15977..16276)
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
ORIGIN
        1 gtgctacacc aagcattgtg tattacgata tagttaatag ttgatagttg attgtatgct
       61 ttttgtagct tgatattcta tttaccaaga agaaacaaga agtgataaaa acaacaagag
      121 agcagtagta agagtatttc gagtgtgaaa aaagtcgcta ctggcactct atttatatgt
      181 gataggcatg ctatagcttt accaaaaagt gaaccccatt tctatgctct cctctgcctt
      241 tttttttttt tttttttcat tctctcaatc tgaaattctc ttatttctcc aacttataag
      301 ttggagatgc ccggtgttcc ggcagaggag atcagtctcg tgaagtggat ggtttcccgc
      361 ctgcgggcaa aacgtcataa catttttatg agcgaaagcc gttaatgaag acaaaatccc
      421 ttaattaaaa cattagaatg gtgattagaa aggcaggatt aatcagttac acaggctgta
      481 accggagaga cggatcataa ggcaattttt agatattaat taatctagaa ctagtggatc
      541 ccccgggctg caggaattcg atatcaagct tatcgatacc gtcatttaaa tgcttgctac
      601 gatcttgggc ataagcctgg ttcaataccc gcgcttaaag ggtaatttcg aaaacagcga
      661 gtgtccggtg tcccaaatgg ttacaaagtg caccctgtac gctagcacga cggttgccac
      721 ctcatgccca ttcttggata gacagaaaca ggattccctc gccaattttc cagcttcttt
      781 gcgttgtata ttagggataa cagggtaatg gcgcgccgag gttttcaccg tcatcaccga
      841 aacgcgctca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
      901 tttataggtt aatgtcatga taataatggt ttcttagtat gatccaatat caaaggaaat
      961 gatagcattg aaggatgaga ctaatccaat tgaggagtgg cagcatatag aacagctaaa
     1021 gggtagtgct gaaggaagca tacgataccc cgcatggaat gggataatat cacaggaggt
     1081 actagactac ctttcatcct acataaatag acgcatataa gtacgcattt aagcataaac
     1141 acgcactatg ccgttcttct catgtatata tatatacagg caacacgcag atataggtgc
     1201 gacgtgaaca gtgagctgta tgtgcgcagc tcgcgttgca ttttcggaag cgctcgtttt
     1261 cggaaacgct ttgaagttcc tattccgaag ttcctattct ctagaaagta taggaacttc
     1321 agagcgcttt tgaaaaccaa aagcgctctg aagacgcact ttcaaaaaac caaaaacgca
     1381 ccggactgta acgagctact aaaatattgc gaataccgct tccacaaaca ttgctcaaaa
     1441 gtatctcttt gctatatatc tctgtgctat atccctatat aacctaccca tccacctttc
     1501 gctccttgaa cttgcatcta aactcgacct ctacattttt tatgtttatc tctagtatta
     1561 ctctttagac aaaaaaattg tagtaagaac tattcataga gtgaatcgaa aacaatacga
     1621 aaatgtaaac atttcctata cgtagtatat agagacaaaa tagaagaaac cgttcataat
     1681 tttctgacca atgaagaatc atcaacgcta tcactttctg ttcacaaagt atgcgcaatc
     1741 cacatcggta tagaatataa tcggggatgc ctttatcttg aaaaaatgca cccgcagctt
     1801 cgctagtaat cagtaaacgc gggaagtgga gtcaggcttt ttttatggaa gagaaaatag
     1861 acaccaaagt agccttcttc taaccttaac ggacctacag tgcaaaaagt tatcaagaga
     1921 ctgcattata gagcgcacaa aggagaaaaa aagtaatcta agatgctttg ttagaaaaat
     1981 agcgctctcg ggatgcattt ttgtagaaca aaaaagaagt atagattctt tgttggtaaa
     2041 atagcgctct cgcgttgcat ttctgttctg taaaaatgca gctcagattc tttgtttgaa
     2101 aaattagcgc tctcgcgttg catttttgtt ttacaaaaat gaagcacaga ttcttcgttg
     2161 gtaaaatagc gctttcgcgt tgcatttctg ttctgtaaaa atgcagctca gattctttgt
     2221 ttgaaaaatt agcgctctcg cgttgcattt ttgttctaca aaatgaagca cagatgcttc
     2281 gttcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     2341 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     2401 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     2461 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     2521 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     2581 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     2641 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     2701 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     2761 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     2821 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     2881 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     2941 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     3001 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     3061 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     3121 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     3181 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     3241 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     3301 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     3361 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     3421 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     3481 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     3541 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     3601 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     3661 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     3721 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     3781 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     3841 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     3901 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     3961 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     4021 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     4081 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaatt
     4141 aatagggata acagggtaat gtgtagatca agccctgaat tgcaaacgat tatgaagtga
     4201 cacgggtgtt acggcttgcg tccagccaga gttttttagg agcatcaacg ttgcccctgg
     4261 ggtgtagtaa ctcaccagtg gccgcccacg tgatagtatg ctccataacc tccaactagt
     4321 ttcgtctgta aggcgggcaa aatatatctt gtttgagtct cgccaagcgc gcaattaacc
     4381 ctcactaaag ggaacaaaag ctggagctcc accgcggtgg cggccgcata gcgccgatca
     4441 aagtatttgt tacgacaata tcgaataacg atggcttccc cgctatactt aagaggatct
     4501 cgcaggcttt acgtaaaaga gagatatact ttaaaattga cgattccaat acttcaattg
     4561 gtaaaaaata cgcccgtaac gatgaattgg gaacaccatt tggtatcacc atagatttcg
     4621 aaactataaa agaccaaacg gtgacgttaa gagaaagaaa ttctatgagg caagtaagag
     4681 gcactattac tgatgtgatt tcgaccattg acaaaatgct tcacaaccct gatgaatcgg
     4741 actgggataa atcaacattt ggactgtcgc ctgttaagat ataactgaaa aaagagggga
     4801 atttttagat actgaaatga tattttagaa taaccagact atatataagg ataaattaca
     4861 aaaaattaac taatagataa gatttaaata taaaagatat gcaactagaa aagtcttatc
     4921 aatctcctta caacttttgg gatgtggtct cctccgtctc gttcctttcc cctgtctcag
     4981 cggccttttc acccgagtct actacagctt tctcagcatc agccacttga acctttgcca
     5041 gctctttacc tagcggttca tctggttcgt tgtgcgcaac tccacccgag tttactacag
     5101 ttttctcagc atcaaccact tcaacctttg ccggctcttt acctagcggc tcatccggtt
     5161 cgttcgcaac tccagatgtt tctccagtct tgatggtatt gtcaatcgag cctgcaccag
     5221 ccttctcagg atcaagaacc tctcccttgg tctgctcgtc gtccaaaaac ctatcaggca
     5281 gataatgtac aagcccaaaa gtggcaagag ctccgacagc caaccaagca ctggcctgga
     5341 taaacgttac agagaaacct ttgctatacg cttgtcgtat gatgaggctc gttgccggat
     5401 ccagcgaagg taatacacta gtcgggctct tcaaaatgcc ttcaagcaat cctggagaac
     5461 tggccaatcg cgcattactg ccgagagact tttccaccga atcatttaga atcgccgtcg
     5521 aaattgcaat tgcaaccaat ccacccaatt gccgaatgaa cgtctggagg cccgtagcag
     5581 ccgccaaatc tcgtctctcg gcgcagaact ggatgatcaa ggtcgacgcg gtgatggtga
     5641 caccgataga aaagccaacc atggcttcga atccgtaaat atgggaaaac ttggttggga
     5701 cggtgacagt ggacaatagg ccagcaccga tagcaccgat ggacgaggcg aaccacatga
     5761 gaggtcggta acttttggtc agcatgacca tggcgcctgc gaagggactg aggcatccaa
     5821 cgggaaggag aaggggcagc atgcgaacac cggcggtgac aggtgaaaca ccatctacga
     5881 cctgcatgcg ctgcggaagg aagatggcat gcgagaacag accggctcct aatgccagcg
     5941 tgaaaatcaa aataaagcag atgttgcgga ctcggaatag acgtaaagga ataacgggtt
     6001 ctgggtgacg agtttcgacg aacgcgaaga ctggtagaat gcagaaggag atgatgagcg
     6061 gggcgagtac ttggacggat acccatggat atccgtctgt acctccgact tggagcgaga
     6121 acgcgaggag aacactggca gccagtaaca ggaaactgcc tatccaatct atcctttgac
     6181 tgatcttttg gatgctcata gggccgagat ctggatcact cagagcgtag aacaacaaag
     6241 atgatgcaac agctccgatg ggtaagttga cgaagaaaat ccatccccaa tggacgtgct
     6301 gaacgattgc accaccaatg agtggacctg acacactgct tgcggcgaaa actgtcccaa
     6361 tgatgggtgt gtatgcacca actttctccc taggaacaat cgtactgatc gtcaccacaa
     6421 cgccagagta gattgctgaa cctccgattc cctgcaacgc cctaaacaca atgagctgtg
     6481 tcattgactt ggcccctcca cacgctccgg accataacat aaagaacaga gtggatacaa
     6541 tcagaactgg ctttctcccc acgatatctg tgagcttgga gactactgga agaaacgccg
     6601 tgtaggtcaa caggtaggag gtaacgagcc aactggtttg ctgatactca ttaaagtccg
     6661 cgccaatcgt gggtaacgcg accgcgacga tagttgagtc caacgcagag ataaagaggg
     6721 tcagcgcgag tccaagcgca acgagatatc tcttgtgacc gtaaatataa taggcctcag
     6781 cgggtatatc cggcattgca acagacatag tgtattataa tataattgac agttgacagt
     6841 tgattgtatg cttgttgtag cttgatattt tatatactaa ggagaaacaa gaaagaagta
     6901 ataaaaacgc tggaagagca gtagcaagcg tatttcgaag atgaaaaaaa gccattatcg
     6961 gcactctatt tatatgtaat aagcatactg taactttgcc aaaaagtgaa ccccattttt
     7021 atgctctcta cctttttttt ttcatcctct gaaaatctga aattctctta tttctccaat
     7081 ttatgagttg gagatggccg gtgttccggc agaggaggtc aggcccgtga agatgggcag
     7141 cttcacgcct gcgggcaaaa cgtcatactt ttttttgggc ggaagccgtt gatgaagaca
     7201 aaatccctta attaaacagt agaatggtga ttagaaaggc aggattaatc agttacacag
     7261 gccgtgactg gagagacgga ctataaaggc aatttttaga taagactagc gaaaagccaa
     7321 ttagtgtgat actaagtgct ttatcgaaaa tccgtgatgc cggtccttca ggcatcaaat
     7381 ttcagtggcc taattattca cagagttctc atgtgacaag tattgatgat agtagtgtca
     7441 gttatgcttc aggttatgtt actataggat aatgatcacg gctaaaacgg tcgaatgtaa
     7501 gcatatatct ttcgattgta taattgttcc caaatactac agcatctcaa ggaaaaaaaa
     7561 acaaaaactt ccaaaaaaat cgaatccctg aggaatcttt aatacatttt caatctattt
     7621 aagttttata aacgtgtata tgagatgtca tgagcatgaa ttattaataa taaaaactaa
     7681 atcattaaag taacttaagg agttaaattc agtagccccc atcatcagtc gtacgatgcg
     7741 acgccatctg cctcctctgt gtctcctcaa gcttgggaca attgctaatc ccatggccca
     7801 ggccaccaca aacggggcat cccttcaacg atccgccttg agcagcacga ggatcctcaa
     7861 tagtagcaag gaacggagga actttttgcc ccgcctccat aagcaaatac ttgagatcca
     7921 acaacgtctg ctccggtgta ttcatattca caaatgtcgt tgcaataccc gtctttccac
     7981 tacgacctgt acgtccgatt tgatgcacgt agtcttcaat ttctttaggc atcgaaaaga
     8041 tgatgacatg ctgaatatcg ttgaagtcga gtccttttga ggcgacgccg gatgctacca
     8101 tgacgtcttt tgcacccgac ttgaaggact tgatagcgta ttgacgctct tcttggctct
     8161 tggaaccatg gatcgctacg gcctcaacac ctttgagcaa tagatactct tgaatatcgt
     8221 cgacctcatt cttgttctca ctgaagatga ttaccggtgg aggtgttttt tgtaggcact
     8281 ccaacaagta caccatcttg gcttcttgct tgacgtattc gacgacttgc aggacatcca
     8341 agttggcagc accagcacgg cctacattga cgagaaccgg tttgatgagg gattgttgcg
     8401 caaaatcctg gatcttccga ggcatcgttg cggagaagag taatgtttgt cgttgacgct
     8461 tgaaaaagct cataatattc cgcacatcat cttcaaaacc cagatcaatc atccggtcgg
     8521 cttcgtccat gcaaagatat ttgcagttgt taaatgtaaa acgcttcttt tccagcatgt
     8581 cgataagcct tccaggagta gccaccacaa tgtgcagacc tttgctcaat gtatggcttt
     8641 gttcattcat ggaaatgcca cctatacata acagggtatt cagccgtgga tatttgccgt
     8701 cctgagcgag agcgtcggtc catgtgagca cgttttcgta ggtttggttt gctagttctc
     8761 gggatggaca taggatgaca ccgacgggcc cttcgcctcg aacgagagga agtttggctt
     8821 cttcctcgat tgcgagcatg atcaggggca tgcagaaagc tagcgttttc cctgaacctg
     8881 tgaatgctat tccgatgaga tctctcccag cgaaggccgt cggaataccc tgaagctgaa
     8941 ttggtgtcgg ttttgtaatg cgcttcgact tgagatgctg caaaatggag tcgggtatct
     9001 tcatgtcgct gaagtgttcg attggaggcg gaacatcttc tccttctaca atgatatggt
     9061 atttctcgcg aagctgttcg tgctgttcct ttgtcttttc gcgaatatat cttgggggtc
     9121 gccagctagt ttttaaagat tcggtatatt gtatgccttt ggccagctct ctgtctgaag
     9181 ctagcttcct gcgaatcttt atagcctcca ggatctcttc atccacttcc cgtgcctttt
     9241 cctcctgagt cttcttcgcg tcttcagcgg ccttcttgga atgcacctct tgagcttcta
     9301 gtagtaaggt tctctccttc ctcgactttt ctctttgtcg ttcttcttcc tggttctctt
     9361 cttcgcgttc ctcctcctct ctctgcttgc tattccggtc tctctccgtc gaagcaccca
     9421 gagatgagag ttttgcgagc tttgcttgtc gtctttgggc tatcgggata tacggttcgt
     9481 aattactatc gtcgtcgagc ctatatgtcg gagaggggga gcgctgaatg cgacgacgtt
     9541 ttgcaggttg ttccattttc ttaattcttt tatgtgcttt tactactttg tttagttcaa
     9601 aacaatagtc gttattctta ggtactatag cataagacaa gaaaagaaaa ataagggaca
     9661 aataacatta gcagaagtac ggtatatttt actgttactt atatactttc aagaagatga
     9721 gttaaatcgg tagccagtgt agaaaaataa taataagggt catcgatcct tcgcatttta
     9781 ttatccaatt aaagatacga atcacggcaa actatattca aagctcatag ataatcgtcg
     9841 taaggctgac actgcagaag aaaagtcata atttgaatac tagccggtat gaaactgtga
     9901 ttgattaacc tggggttacc taaagagaac ataagtaata ctcatgacag aatcaaaaca
     9961 caatacaaaa tttatccgaa cctcggcccg actgccggct cgccgggaaa ggggacaacc
    10021 gcttctatcc gtcgactaac ttcatcggcc caatggaggt atcatctcca tctcccatat
    10081 gcatatcact gtggtcgtgg cccgcatttc cttccatctt gtgattcatg ctatccatct
    10141 tttttgagta tccaattaac gaagacgtta ccagctgatt gaaggttctc aaagtgactg
    10201 tactccatgt tttcttatca tccatgtagt tatttttcaa actgcaaatt caagaaaaag
    10261 ccacgcgtgt gcaccttttt tttctccttc cagtgcatta tgcaatagac agcacgagtc
    10321 tttgaaaaag taacttataa aactgtatca atttttaaac ctaaatagat tcataaacta
    10381 ttcgttaata taaagtgttc taaactatga tgaaaaaata agcagaaaag actaataatt
    10441 cttagttaaa agcactctag gcaaccgcca tgagcaagct atcaacgtgt tggtgggcag
    10501 gaagaacagg tttgggctgc agaataggaa gagtggcgtc gaacagagac ggctgtcggt
    10561 ctggagcaga agcatgctca aggctggcag tggaaaactc aaaaccacta tcccaatctt
    10621 ccttcgactt gacgagcgat gacgaacggc cagggtgcgg ggttggtaac tcacgaacag
    10681 tagtcgttgc tacctttgga gcaagagcag acggcggcgg ggtgtgaggt ggtgatggaa
    10741 gtgatggtga cgatgccgga gaaggtgctt ggatgtgctg gacagatgcc ctgtattcat
    10801 ccttggctgt aaggaagata tcttgtaacg taaggccaat gtggtcatga cattccgtga
    10861 atgcggcatg tttatgaagc atgtccagcc atgcgggcca aatgccttct gcaatgaaga
    10921 gattgtagtc tagcaatgag aggccctggt gttcccacaa tctcgcgtct tccagagaga
    10981 cttccatgca ttcagcgatc tttgagaggg accaaggaat atcttctagc cagataaagg
    11041 caaagtggag agcgagtagg tatgacctat acatgatggt agcgcgatcg cgatcactaa
    11101 atgtttcgcc gattttgatg ccacgaatga gcctctccag gtagataaga gcggcaagag
    11161 cgatcgagtt gtgcgggtcg aggtagtcca agacgctaac gagtttcccg ctcatttcct
    11221 tcatgataat atgattttcg acaaacctga actcaggcgt agaaaatctg tcacacagat
    11281 attcaccaat ccagaggccc agtgcaacct tgcttgccca gtcacggcaa ggaagctctg
    11341 gaggtgtggc tgggggagag gtagttgtgc ttgtgggctt tccctctgtg tgtgccgatg
    11401 tggtagaaaa tggaagcggg aaatctgtat ctgtctggat tctacggcgc ttgggctctg
    11461 gaatgaccag ataactgtct cgagatggag gctgtccgtc cttggtttca tcacgggatc
    11521 gcttcacacc acatttgggc ccagatgccg atggaggaat ggaggcagac gaagtggata
    11581 cgcaaccgct tccggagaga atggaagaga gaacagagcc acgacgactg gtgttcggct
    11641 gcgatggctc gggctgttgc tgtgcgtcgg ggaagaaata cgggaggtga ctgactgatt
    11701 ccatgttgtt attttattat ggaataatta gttgcgtgag tttggttttt tttttttatt
    11761 tttgagttaa acaaatataa ttattgttta agctgaaata aagatatata tgtatatctg
    11821 ggaaaggctg ttggttatct gtcttacttc tgtatattta agaagtaagt agaaatactg
    11881 attacacatg gaagagaagt tacacacatt acaaagactt ctctgccctt atataccttt
    11941 tttttgtggt cttctatact ctgcccaatg ttgctatccc cagccaagcc ttacttaata
    12001 cacaaacgta ccattgtcca accagaagcg tctaagcttt acaatcctgt ttgggccctt
    12061 tatccgccta tccataatgt taagcagcct aaaacttgct agtctagccg gcatctttcc
    12121 cttgtataac ttaaaatacc cggagcttca gtgatatgta gaacacagcc gcgccggatg
    12181 aaaggaagct cggttttttc ctatcctgat gcggtatttt ctccttacgc atctgtgcgg
    12241 tatttcacac cgcatagggt aataactgat ataattaaat tgaagctcta atttgtgagt
    12301 ttagtataca tgcatttact tataatacag ttttttagtt ttgctggccg catcttctca
    12361 aatatgcttc ccagcctgct tttctgtaac gttcaccctc taccttagca tcccttccct
    12421 ttgcaaatag tcctcttcca acaataataa tgtcagatcc tgtagagacc acatcatcca
    12481 cggttctata ctgttgaccc aatgcgtctc ccttgtcatc taaacccaca ccgggtgtca
    12541 taatcaacca atcgtaacct tcatctcttc cacccatgtc tctttgagca ataaagccga
    12601 taacaaaatc tttgtcgctc ttcgcaatgt caacagtacc cttagtatat tctccagtag
    12661 atagggagcc cttgcatgac aattctgcta acatcaaaag gcctctaggt tcctttgtta
    12721 cttcttctgc cgcctgcttc aaaccgctaa caatacctgg gcccaccaca ccgtgtgcat
    12781 tcgtaatgtc tgcccattct gctattctgt atacacccgc agagtactgc aatttgactg
    12841 tattaccaat gtcagcaaat tttctgtctt cgaagagtaa aaaattgtac ttggcggata
    12901 atgcctttag cggcttaact gtgccctcca tggaaaaatc agtcaagata tccacatgtg
    12961 tttttagtaa acaaattttg ggacctaatg cttcaactaa ctccagtaat tccttggtgg
    13021 tacgaacatc caatgaagca cacaagtttg tttgcttttc gtgcatgata ttaaatagct
    13081 tggcagcaac aggactagga tgagtagcag cacgttcctt atatgtagct ttcgacatga
    13141 tttatcttcg tttcctgcag gtttttgttc tgtgcagttg ggttaagaat actgggcaat
    13201 ttcatgtttc ttcaacacta catatgcgta tatataccaa tctaagtctg tgctccttcc
    13261 ttcgttcttc cttctgttcg gagattaccg aatcaaaaaa atttcaagga aaccgaaatc
    13321 aaaaaaaaga ataaaaaaaa aatgatgaat tgaattgaat gagttatgtc cttcgcgcac
    13381 tgattcatct ttgaaactaa atttgtatat tgtttgtcag aaataggacc tgatatcctc
    13441 cttttaaaca gttgatgaga acctttttcg caagttcaag gtgctctaat ttttaaaatt
    13501 tttacttttc gcgacacaat aaagtcttca cgacgctaaa ctattagtgc acataatgta
    13561 gttacttgga cgctgttcaa taatgtataa aatttatttc ctttgcatta cgtacattat
    13621 ataaccaaat cttaaaaata tagaaatatg atatgtgtat aataatataa gcaaaattta
    13681 cgtatctttg cttataatat agctttaatg ttctttaggt atatatttaa gagcgatttg
    13741 tttaaaacgg agatccttca atagggcaca cagtgcctgg ctctgggaga gtcccgttga
    13801 caaaatactc gcgaattatg ttataggtgc agaccgaggg ccctgccagg attgaagtat
    13861 ggccagcgca atcctgctga agcacgactg atccggggaa accttttgaa accttgtgtg
    13921 cagcagagag gggtgtgatg ggatcagcag tatttcctat gaggaggatc ggatgactcg
    13981 tgtttcccga aataggacct ctgaagggcg cctttggaat gttgggccaa ccactgcatt
    14041 taattctcat ggctgatgac atactacccc attccgatac tttcagggac tcctcatagt
    14101 gctgttgtgc atgttccagg ctaggtggca cagcgtcacc gtcattgcag actatcacag
    14161 tctgagactc atcgttgttt gcaaaagaca ccggagcgtc gtctgaatcg ggggaagatg
    14221 atggcactag cccgctcatc tgagccaacg tggtaccatt tcccttttcc aagtcggcca
    14281 aggcagttgc caaagtggac caccgggtca atggatagta cagcgcattg aatattacga
    14341 accgcagtga tgcataatca atgaggccag cgccgcgtac cggtaccgga gctcggataa
    14401 tggactggta taatctgttg agtctttctt cgatggcttc ggccgacgac tcatagaagg
    14461 agcatttttc tgctcccgca tcatggcagt ctttgaagaa ccacttcagg gtggcatcgg
    14521 tatcggaaac gacatccttc catcccatgg tgtagtagtc atcttgaacg tcaagcactc
    14581 cgtcgattac caagcgttgg accttgtctg gaaacatagc tgcaaaggtc gaaccaagag
    14641 ccgtgccata tgagatgccc caatactgga gcttctctct accgtaagcc tccgtaatgc
    14701 gaagcatgtc tcgtgctgtg tggtcagtgt tgatataagg caggaaatcc ccatgacgtt
    14761 cttcagcaag ttgggcgatt atctttgcac gagcccaata actcgcaaca ttgtcttgca
    14821 aagaggagtt gagctcccaa attccagacc tttgccatga cagacgctca tcaagagttt
    14881 catagattgt cacgcgtgga gtggagcgcg atacacctct ggggtcgaat cccactaaat
    14941 cgaactgagg ccccaagatt tctcttaatt ttgtgcccaa cgaaactata tagtcgacac
    15001 cactaccgcc aggtcctccg ggattgaaga ggataggacc aagataatcc gtactatcgg
    15061 cagagacgtt ggctttcatg cgcacgagtg ctatggcagc agattgtcct tctgggtcgg
    15121 cataattcaa tgggacctgg aagcgaccac attcaaggcc tccgtcgtag catggcaccc
    15181 aggtcaagtt ctgagtaggt gtgatagaca accaggacga ctcgttccat gtagcttgag
    15241 atacagcacc tctagtaagg catacaagta tgcaagctgt ggtaagtaag gtttttgttg
    15301 tccggttcat tttgtattgt attttgagga tagagttgac agttgatagt atgcttaata
    15361 tagcttgata tacttatata ctaagtagaa acaagagaat tgtaacctga aaaagggaga
    15421 agaccactaa caagacagat tttgagataa acagggtggc aatggccact ctatttatac
    15481 gcacccagta tgcttgatct gaccgactgt gtgtgagtgt gtgacataag ggaactaact
    15541 ccatttgcgc tgtgctgtac cttttttttt tcttccatta aacctgcgat gctcttaatt
    15601 tccccaactt gcaagttgga gatggccgtt gttccggtgg taggtcaggc cggtggaaaa
    15661 tgggtagttt cgtgtctatg ggttaaaaaa aacgtcattt ttgggcgcat gccgttgatg
    15721 gagacaaaaa tcccttaatt aagcatcagc ataggtgatt agaaagaaag gtttgaccag
    15781 ataaaggctg tgtcactgga gaactggatc gtgagggttc aataattgaa attatagggt
    15841 ggacgtcaag acgaaaagtg aaaaattaca tccgtataga attatataac ttgatgagat
    15901 gagatgagta aatgacagaa gaattaccgt ttcatcattg aacttcgatc atttcaatgc
    15961 tggcatgcga aggaaaatga gaaatatcga gggagacgat tcagaggagc aggacaaact
    16021 ataaccgact gtttgttgga ggatgccgta cataacgaac actgctgaag ctaccatgtc
    16081 tacagtttag aggaatgggt acaactcaca ggcgagggat ggtgttcact cgtgctagca
    16141 aacgcggtgg gagcaaaaag tagaatatta tcttttattc gtgaaacttc gaacactgtc
    16201 atctaaagat gctatatact aatataggca tacttgataa tgaaaactat aaatcgtaaa
    16261 gacataagag atccgcctag agtctagata aatctcttcc ccacacacca cgaataatcc
    16321 aatcaatcat caacaacgct ctagtcctag ccgacacttg ctcattccaa tatacacttc
    16381 tccacgcgta catggcagcc aatccacccg tgaacgacca aggtcccaaa tcaaacacag
    16441 cagcgttgcc aatatatgcc aacatgccca gatggtgata tttgaatggc gtccctccaa
    16501 catccattgc ccttgtctgt tggaactctc tttgccgagc gagcctgtgg agcttcttcc
    16561 cgatgtattt gccttgttga gaagctacct gcgcagtagc aggtaaagcg gtgatcttgt
    16621 tcccgaggtc ttccaacaac cggacgagtt cgttgagggt taaactgtta tccgaatctg
    16681 tgtcatacat ctcgaagagt tctctaactt tgctgacatg gttctcagcc atgggtatct
    16741 ttttcttgat cctcttgacc attacttccc actctccgaa atctatcttg ccgttcttgt
    16801 ctttatctgc ctcgtccacc aattccatga agtggctcac gagcgaagtt tcaatcgtag
    16861 cacaatcccc gacagcatag atctctccga ccggtgcacc cttcacccgt agatgcgcat
    16921 ccgtctcaat ggccttttta tggacttgat tgggtagcaa ctctgtgact ctctttgtaa
    16981 agggattcat cgctattccc gtcgaccata gaacgaaatt ggtagggacc gtgtgttgag
    17041 taatttgacc agtttcattg tctcgcgctg tgaaaatgac ttgtgtaggc gttacggcgc
    17101 tcacgcgtga gttcgtaatg agattgacac catcgcgttc aaatttctcc tcggcatatt
    17161 ttgatatggc ttccgagtaa gtgttcagga tatgttctct agactggata acatgaatgg
    17221 aaacctcttc tcggcagatc ttggggaagt aattcatgat atcctcttga cagaaatcat
    17281 atatttccgc ggctgtttca acgccagtag gaccaccacc acaaacgaca aagctcaaca
    17341 gcctctttct ctcttcagga gtagtagtcg gcagactagc gatttcaaag ttgtccatga
    17401 tgcgtctccg catagcttga gcatctgaga tggtcttgag ctggaaacag ttctcgaggc
    17461 cggggacacc gtgagtactg gaggtcgatc cgactgcgat aacgaggtgg tcgtaaggga
    17521 catatatacg ctctttagtg ccattagagc tccaagtttc aacttcaagt agtttatcct
    17581 tgatgcaaag gtccacagct tttccagcaa caaaatgtcc gcgcagacgg gcaatgatct
    17641 ttcgaatagg ctcgataagt gaacgaactt gaacggtacc aacagcagcg gagggtagca
    17701 atggcgtgaa ggtggtgaaa gtttctgcgg atacgacggt tacatggtag tctcctggat
    17761 gaagcgttcg tagcgttcca atagcccccc aaccaccacc gacgataacc aactttggtt
    17821 tctcagccaa tttcctcgtc tcctcatcct cctggtcatc catctgcact ttgactatcg
    17881 gaagattctt gggccctcct cgctctggat gcaacgccaa tggatgtact ggtacccgtt
    17941 ccacgtgtcg ttcgttgtag gtaaacgcgt cgtgaatgaa tatcccagca cctacgacga
    18001 caaggccaaa gactgaagat agaccgacat aagcagtgac ttttgcaacg gttaatgccc
    18061 ggcgaataat ggtagatcga ggtttaggtg gtggaatttc cgtggaagtt gagttgaacc
    18121 gggcatgccg agttgaagaa ggaattgtcc agggatttcg tgacgaacga tggagaaata
    18181 aattctgccg agagcgagcg ccggcgc
//
