LOCUS       sequence-297990-       12628 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12628)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 12628)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU42.2-2.2
FEATURES             Location/Qualifiers
     source          1..12628
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     506..522
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     506..522
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(864..882)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      923..2265
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    1255..1302
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        2292..2396
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2397..2465,2466..3257)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2615..2634)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3428..4016
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3917..3936
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4353..4373
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4355..4373
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(7076..7879)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(7880..8100)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     terminator      complement(10793..11092)
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
ORIGIN
        1 gtattacgat atagttaata gttgatagtt gattgtatgc tttttgtagc ttgatattct
       61 atttaccaag aagaaacaag aagtgataaa aacaacaaga gagcagtagt aagagtattt
      121 cgagtgtgaa aaaagtcgct actggcactc tatttatatg tgataggcat gctatagctt
      181 taccaaaaag tgaaccccat ttctatgctc tcctctgcct tttttttttt tttttttttc
      241 attctctcaa tctgaaattc tcttatttct ccaacttata agttggagat gcccggtgtt
      301 ccggcagagg agatcagtct cgtgaagtgg atggtttccc gcctgcgggc aaaacgtcat
      361 aacattttta tgagcgaaag ccgttaatga agacaaaatc ccttaattaa aacattagaa
      421 tggtgattag aaaggcagga ttaatcagtt acacaggctg taaccggaga gacggatcat
      481 aaggcaattt ttagatatta attaatctag aactagtgga tcccccgggc tgcaggaatt
      541 cgatatcaag cttatcgata ccgtcattta aatgcttgct acgatcttgg gcataagcct
      601 ggttcaatac ccgcgcttaa agggtaattt cgaaaacagc gagtgtccgg tgtcccaaat
      661 ggttacaaag tgcaccctgt acgctagcac gacggttgcc acctcatgcc cattcttgga
      721 tagacagaaa caggattccc tcgccaattt tccagcttct ttgcgttgta tattagggat
      781 aacagggtaa tggcgcgccg aggttttcac cgtcatcacc gaaacgcgct caccgtcatc
      841 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
      901 gataataatg gtttcttagt atgatccaat atcaaaggaa atgatagcat tgaaggatga
      961 gactaatcca attgaggagt ggcagcatat agaacagcta aagggtagtg ctgaaggaag
     1021 catacgatac cccgcatgga atgggataat atcacaggag gtactagact acctttcatc
     1081 ctacataaat agacgcatat aagtacgcat ttaagcataa acacgcacta tgccgttctt
     1141 ctcatgtata tatatataca ggcaacacgc agatataggt gcgacgtgaa cagtgagctg
     1201 tatgtgcgca gctcgcgttg cattttcgga agcgctcgtt ttcggaaacg ctttgaagtt
     1261 cctattccga agttcctatt ctctagaaag tataggaact tcagagcgct tttgaaaacc
     1321 aaaagcgctc tgaagacgca ctttcaaaaa accaaaaacg caccggactg taacgagcta
     1381 ctaaaatatt gcgaataccg cttccacaaa cattgctcaa aagtatctct ttgctatata
     1441 tctctgtgct atatccctat ataacctacc catccacctt tcgctccttg aacttgcatc
     1501 taaactcgac ctctacattt tttatgttta tctctagtat tactctttag acaaaaaaat
     1561 tgtagtaaga actattcata gagtgaatcg aaaacaatac gaaaatgtaa acatttccta
     1621 tacgtagtat atagagacaa aatagaagaa accgttcata attttctgac caatgaagaa
     1681 tcatcaacgc tatcactttc tgttcacaaa gtatgcgcaa tccacatcgg tatagaatat
     1741 aatcggggat gcctttatct tgaaaaaatg cacccgcagc ttcgctagta atcagtaaac
     1801 gcgggaagtg gagtcaggct ttttttatgg aagagaaaat agacaccaaa gtagccttct
     1861 tctaacctta acggacctac agtgcaaaaa gttatcaaga gactgcatta tagagcgcac
     1921 aaaggagaaa aaaagtaatc taagatgctt tgttagaaaa atagcgctct cgggatgcat
     1981 ttttgtagaa caaaaaagaa gtatagattc tttgttggta aaatagcgct ctcgcgttgc
     2041 atttctgttc tgtaaaaatg cagctcagat tctttgtttg aaaaattagc gctctcgcgt
     2101 tgcatttttg ttttacaaaa atgaagcaca gattcttcgt tggtaaaata gcgctttcgc
     2161 gttgcatttc tgttctgtaa aaatgcagct cagattcttt gtttgaaaaa ttagcgctct
     2221 cgcgttgcat ttttgttcta caaaatgaag cacagatgct tcgttcaggt ggcacttttc
     2281 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     2341 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     2401 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     2461 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     2521 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     2581 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     2641 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     2701 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     2761 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     2821 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     2881 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     2941 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     3001 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     3061 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     3121 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     3181 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     3241 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     3301 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     3361 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     3421 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     3481 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     3541 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     3601 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     3661 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     3721 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     3781 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     3841 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     3901 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     3961 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     4021 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     4081 ttcctgcgtt atcccctgat tctgtggata accgtattaa ttaataggga taacagggta
     4141 atgtgtagat caagccctga attgcaaacg attatgaagt gacacgggtg ttacggcttg
     4201 cgtccagcca gagtttttta ggagcatcaa cgttgcccct ggggtgtagt aactcaccag
     4261 tggccgccca cgtgatagta tgctccataa cctccaacta gtttcgtctg taaggcgggc
     4321 aaaatatatc ttgtttgagt ctcgccaagc gcgcaattaa ccctcactaa agggaacaaa
     4381 agctggagct ccaccgcggt ggcggccgca tagcgccgat caaagtattt gttacgacaa
     4441 tatcgaataa cgatggcttc cccgctatac ttaagaggat ctcgcaggct ttacgtaaaa
     4501 gagagatata ctttaaaatt gacgattcca atacttcaat tggtaaaaaa tacgcccgta
     4561 acgatgaatt gggaacacca tttggtatca ccatagattt cgaaactata aaagaccaaa
     4621 cggtgacgtt aagagaaaga aattctatga ggcaagtaag aggcactatt actgatgtga
     4681 tttcgaccat tgacaaaatg cttcacaacc ctgatgaatc ggactgggat aaatcaacat
     4741 ttggactgtc gcctgttaag atataactga aaaaagaggg gaatttttag atactgaaat
     4801 gatattttag aataaccaga ctatatataa ggataaatta caaaaaatta actaatagat
     4861 aagatttaaa tataaaagat atgcaactag aaaagtctta tcaatctcct tacgtggaat
     4921 ccggggcggt caagcggacc aataacgcca tctccagtgt acggggtttg atcgtgcatt
     4981 tgaaaggaag tgggcggctc tggaaacccg acgtatactg tgccgtgggc tcttcgacac
     5041 gcccgtcggc gtcgagcgct ttctcaatgt cgaacgccgc gacggtggta gcgatcatga
     5101 tccagagcgc gtcgatggca aagaaccgac ccggacaaat acgtctgccg aagccgaaca
     5161 cggcgtccgg gtcggggtta gggtccgagc ccatgaagcg gtttgggtcg aattccgagg
     5221 tgtgggggta aacgtgctca tcgtgcagta tggcccagat atttccggcc actatagatc
     5281 ccttgggaat tctgtaaccg agcccaacgt catcttgggt gacccgatgt ggaatgccca
     5341 gcgggacggg cggcctccat ctaagacact cctttgcgat ggcgcgtata tatggcaagt
     5401 cgtccgagaa gtcaggaagc cggccttcct tggtgactct gctgacctcc ttccgcgcgt
     5461 tctcttgaac gtctggatac aacagcatag ccagaaagaa ggtattcaag gctgcgacgt
     5521 tggtttcggc gccggccccg tagagtaccg atgtactccg cttcgtcgtc atctccacct
     5581 cccgtcgttg ctcagcagtt gcgctatcgt caagttctcc aagaagctcg gagacgatag
     5641 attcctcgtt attgcgcgtc aacatcatcc tcttcgcgtg atcatacgga tcgtgaagca
     5701 agctttcccc gaccttcttg aattcaagcg cgtctctttt aaaccctgtg ccagggaacc
     5761 aagacggaag gtatttgagt tttggaaata cgtccacaag gtaggatccc gggcttatag
     5821 ccgcgtttcc gatctcgttg gctctctctg ccagattgat ccagggatcg ccgttgggta
     5881 acgcgtgtat gtcgtatgtc acattgagga tattagaccc tacagagtag cgtgtatgtt
     5941 taaagagttc tccgggcgca tctagcagag agcgaccgaa tgcgtacgcg gccaaggtct
     6001 gctttcgcct ccattgtttc gacctttcgc tgctaagaac tctctggtac tgaacgcggc
     6061 ttgccttcca tctagcgttg ttggacatta acgctatatc ccagtccgcg tggttcgcca
     6121 gatcgcacat catggtcagc catgggcgat ccgagtatat cgtcgacttc ttatcaagca
     6181 attcagccgc acttcgtgct gtattgatga caactagatg gaatcccaac aattgaatat
     6241 gcaccacgtc tgagcaatat ctgcgccccc attctacata ggttttccat gggtaatgcg
     6301 ttggcatatc caagagattg cctatcaaag ggagtccctt gggccccggt ggcaggggag
     6361 gacgctgctt cgaacgtcga agcgccaaga aaaggagaat cgagaccagc aagacgccga
     6421 ccgctcctat cggaagggat tccatgttgt tattttatta tggaataatt agttgcgtga
     6481 gtttggtttt ttttttttat ttttgagtta aacaaatata attattgttt aagctgaaat
     6541 aaagatatat atgtatatct gggaaaggct gttggttatc tgtcttactt ctgtatattt
     6601 aagaagtaag tagaaatact gattacacat ggaagagaag ttacacacat tacaaagact
     6661 tctctgccct tatatacctt ttttttgtgg tcttctatac tctgcccaat gttgctatcc
     6721 ccagccaagc cttacttaat acacaaacgt accattgtcc aaccagaagc gtctaagctt
     6781 tacaatcctg tttgggccct ttatccgcct atccataatg ttaagcagcc taaaacttgc
     6841 tagtctagcc ggcatctttc ccttgtataa cttaaaatac ccggagcttc agtgatatgt
     6901 agaacacagc cgcgccggat gaaaggaagc tcggtttttt cctatcctga tgcggtattt
     6961 tctccttacg catctgtgcg gtatttcaca ccgcataggg taataactga tataattaaa
     7021 ttgaagctct aatttgtgag tttagtatac atgcatttac ttataataca gttttttagt
     7081 tttgctggcc gcatcttctc aaatatgctt cccagcctgc ttttctgtaa cgttcaccct
     7141 ctaccttagc atcccttccc tttgcaaata gtcctcttcc aacaataata atgtcagatc
     7201 ctgtagagac cacatcatcc acggttctat actgttgacc caatgcgtct cccttgtcat
     7261 ctaaacccac accgggtgtc ataatcaacc aatcgtaacc ttcatctctt ccacccatgt
     7321 ctctttgagc aataaagccg ataacaaaat ctttgtcgct cttcgcaatg tcaacagtac
     7381 ccttagtata ttctccagta gatagggagc ccttgcatga caattctgct aacatcaaaa
     7441 ggcctctagg ttcctttgtt acttcttctg ccgcctgctt caaaccgcta acaatacctg
     7501 ggcccaccac accgtgtgca ttcgtaatgt ctgcccattc tgctattctg tatacacccg
     7561 cagagtactg caatttgact gtattaccaa tgtcagcaaa ttttctgtct tcgaagagta
     7621 aaaaattgta cttggcggat aatgccttta gcggcttaac tgtgccctcc atggaaaaat
     7681 cagtcaagat atccacatgt gtttttagta aacaaatttt gggacctaat gcttcaacta
     7741 actccagtaa ttccttggtg gtacgaacat ccaatgaagc acacaagttt gtttgctttt
     7801 cgtgcatgat attaaatagc ttggcagcaa caggactagg atgagtagca gcacgttcct
     7861 tatatgtagc tttcgacatg atttatcttc gtttcctgca ggtttttgtt ctgtgcagtt
     7921 gggttaagaa tactgggcaa tttcatgttt cttcaacact acatatgcgt atatatacca
     7981 atctaagtct gtgctccttc cttcgttctt ccttctgttc ggagattacc gaatcaaaaa
     8041 aatttcaagg aaaccgaaat caaaaaaaag aataaaaaaa aaatgatgaa ttgaattgaa
     8101 tgagttatgt ccttcgcgca ctgattcatc tttgaaacta aatttgtata ttgtttgtca
     8161 gaaataggac ctgatatcct ccttttaaac agttgatgag aacctttttc gcaagttcaa
     8221 ggtgctctaa tttttaaaat ttttactttt cgcgacacaa taaagtcttc acgacgctaa
     8281 actattagtg cacataatgt agttacttgg acgctgttca ataatgtata aaatttattt
     8341 cctttgcatt acgtacatta tataaccaaa tcttaaaaat atagaaatat gatatgtgta
     8401 taataatata agcaaaattt acgtatcttt gcttataata tagctttaat gttctttagg
     8461 tatatattta agagcgattt gtctaagccc ttggcgtcag aacgaccggc atcgggccgc
     8521 gcgtagccgt gaagtagtct ccgattttct cctcccattc attcgcgttc caaccatcgg
     8581 cgaagcggag ttggaaggta tgcaaaacga ggcagacaac catcttcatc tccatcgtgg
     8641 cgaatttctt tgcgacgcac atagcaggtc cgaacgagaa cgggatgaag gctctagttt
     8701 cgagaatgat cgagttgggg tccttgtact ccacgacctc gttcgatttt cgctgcgcgt
     8761 ggtccagcca gcggtcgggc cagaagcctt cgggagctgg ccagaaatag cgagggtcac
     8821 gttggatcga gtaggtattg atttggacct gcgttccctt gggaatccag tgggttcccg
     8881 ccatcttgcc gtcagtgtac tgcgtactgc gctgcgcgcc actaggtacc ggtggctgca
     8941 agcggagagt ttcgttgata acggcttcga gatatggcag ctcgctttgt ttagcaacat
     9001 ctgttgccgg tgttccacga gggaacgcgg catcgatttc cgcttgcaga cgctcgtagc
     9061 atgatgggtg ccgtaagagg aaccaaaata tgttcgacat cacatgggaa gtcgtgtcgc
     9121 tcccggccgc tatggctaga ataccgtcac tgagagcctc ctcgagaatc ggcgggtggg
     9181 gttcaacgcc agcctcatcg aagatataat ggaacaagtc cttccggagc gaaccttgct
     9241 tgcgacgcag gtcggcgcga ttgatcgcga aatctctgaa cttggagttt ccgaacttgg
     9301 gaagtttggc aaggtagcgg aacagccacg gaacgtgacc agtcatagac gtagactggg
     9361 tgcccttctc gatcaggtgc agaatattgc cctggtcacc gtccctcatc atctcgaagc
     9421 cgccaccgaa cgccatgtca cccatgagat cgaatgagaa gtagcttatc caccgagcga
     9481 gattgacagg gttgttctgc tttcgcaaac catcaatgag ctgaagagct cggctttcaa
     9541 gaatctcttc atagcccttc agcgacgccg agctgaaccc gcggttccag ggcttgcggc
     9601 gacgtgaatg ttcagcgggg ttgcgttctc caatcaagct agtggtggcg ccgggaggga
     9661 cacgcccatc ccaaagagga cccttaggta aaccggtggt gcccaagata ggttggatgg
     9721 catcgacgtc gcagatggat aattcgttgg ggccggtccg gacccaagtg ccaaactgcc
     9781 tatgcatatg ttgatagaag aagtgctgtt tgccttgaga cgtgatgtgc atcatccaga
     9841 atttgctgag tttgttcaga atcggtccgg ggtaccgcgc caaagggtgg aacggcgaga
     9901 cacgataaag tagtaccgac gcgccgagga ctccgtagaa ctcaagaaac agtaggggcc
     9961 atgcgagggc gataccagcg tacttcaacg caaggccagt ggtgactggg acgccgagca
    10021 gcaacgccga cagcggccat gctttctcac attcgaagcg attgaagatg gcgtgcaccg
    10081 ccatagcggc gcctgcgacg gccaccacgt tgtcctccaa acgcattttg tattgtattt
    10141 tgaggataga gttgacagtt gatagtatgc ttaatatagc ttgatatact tatatactaa
    10201 gtagaaacaa gagaattgta acctgaaaaa gggagaagac cactaacaag acagattttg
    10261 agataaacag ggtggcaatg gccactctat ttatacgcac ccagtatgct tgatctgacc
    10321 gactgtgtgt gagtgtgtga cataagggaa ctaactccat ttgcgctgtg ctgtaccttt
    10381 tttttttctt ccattaaacc tgcgatgctc ttaatttccc caacttgcaa gttggagatg
    10441 gccgttgttc cggtggtagg tcaggccggt ggaaaatggg tagtttcgtg tctatgggtt
    10501 aaaaaaaacg tcatttttgg gcgcatgccg ttgatggaga caaaaatccc ttaattaagc
    10561 atcagcatag gtgattagaa agaaaggttt gaccagataa aggctgtgtc actggagaac
    10621 tggatcgtga gggttcaata attgaaatta tagggtggac gtcaagacga aaagtgaaaa
    10681 attacatccg tatagaatta tataacttga tgagatgaga tgagtaaatg acagaagaat
    10741 taccgtttca tcattgaact tcgatcattt caatgctggc atgcgaagga aaatgagaaa
    10801 tatcgaggga gacgattcag aggagcagga caaactataa ccgactgttt gttggaggat
    10861 gccgtacata acgaacactg ctgaagctac catgtctaca gtttagagga atgggtacaa
    10921 ctcacaggcg agggatggtg ttcactcgtg ctagcaaacg cggtgggagc aaaaagtaga
    10981 atattatctt ttattcgtga aacttcgaac actgtcatct aaagatgcta tatactaata
    11041 taggcatact tgataatgaa aactataaat cgtaaagaca taagagatcc gctcaatcca
    11101 cattgcgccg cctcagtcgt acccagtcat gagtgggctt atgcataagc ttctccgtgg
    11161 tctgtagcac aagggactgg ggagtgatgt cgtatcgctg gagtaacgtg gccacgatca
    11221 tcttcagctc aagtatggca aggcttttgc caaggcagcc tctggtaccc gagctgaatg
    11281 ccatgaagta cttggtgagg gacggatctt ctatccagcg ctcgggtctg aatacgtctg
    11341 catcttctcc ccaggctgca cgatcgcgag acatggtcca cgtaggcaca gatacatcgg
    11401 tacctggcgg gaagtagtag tcccggtacg tgagaccccc ctccggtaca atccgaggca
    11461 agcccattcc ggttggtgag tgcaggcgga tagcctcatg gatgcatgcg ttcaggtagg
    11521 gtagatcgtt cagatccgat gtagggccct ggaggccccc tggagagagc gcctcatcga
    11581 gctcttccct gagtttggca agtggtcgag ggttgactga cagcgcatgg atgattgccg
    11641 tgatggcatt cgaagtcgcg tctgtgccag cgattagcag agtcagcgcc tcagatgtaa
    11701 gctcctccac gtccagatgg tttttgtatt ccatgtgggc tgcaatgaga tgacccagga
    11761 tgtcactctt ggcatcgtcg ctgccacttg cgagtcgttg cttgatgcac atccgggcga
    11821 tgtcgcgcaa ctcgtcgctg ctcttgctac ccttctggaa gaatggatcc gggatgaggc
    11881 gtgcaatgta cgcaagagcg ggcgcccagc caataacggt caggtaatgc tcgcgcacgt
    11941 ccgtgttctc tatggcgcct ttcggtgatc ctgcctggag aacgtcggat tccttctcca
    12001 acatgccgag tggcgtccca aaagccagcg tcgacatgat gtcaaagacg aggtagtttg
    12061 accagatgag catgtcgatt ggggccttgt caggctcgcc aggcagcgca gctcgcttgt
    12121 cgagtttcac cagcagttcc tcaaggaacc gatggaccgt tgtcgtatac gaccgaatcg
    12181 ttttaggcgc aaatgcacca gaataattac gacgcttctg tgcgtgctct tgcttgtccc
    12241 gagtgctgaa gatggacggc ttctcggaca ggaaggcgtc gtaaaacggc gacttgttga
    12301 aggccgccat gccttgaccg tagatagagg gtaatagact catgtctgcg atcgagacat
    12361 ggttcggcga gatcctcacg acaggtccat atgtcttatg gacagcatgg atggctttgt
    12421 aacggatgcc gaagcgtgtg tagaacgcct gccataggtt cgaccaacga gcaaggagcg
    12481 gtccagggac cttttgcaag gggtcgcggc tgacggcgac gatatagcca acaactacag
    12541 cggggagaaa gaccagccag gtgtagtcct gcatggcctc aggcaggttg tgtgttgctg
    12601 aggaaaatag ggtggagttc tgcattgt
//
