LOCUS       sequence-298017-        2602 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Diatom promoter for P. tricornutum; uLoop specific syntax; AC 
            overhangs; Lvl 0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Windhagauer , M., Abbriano, RM., Ashworth, J., Barolo, L., 
            Jaramillo-Madrid, A.C., Pernice, M., Doblin, M.A.
  TITLE     Characterisation of novel regulatory sequences compatible with 
            modular assembly in the diatom Phaeodactylum tricornutum
  JOURNAL   Algal Research , Volume 53, March 2021
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Algal 
            Research , Volume 53, March 2021"
COMMENT     Sequence Label: pL0_AC_pSVP
FEATURES             Location/Qualifiers
     source          1..2602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(737..1325)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(817..836)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1416..2207)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cattactcgc atccattctc aggctgtctc gtctcgtctc aggtctccgg agaggtccac
       61 cgcgccaatt gaaacaattg taaatttgca tggtgatgaa tttttagcag ccatcgaatt
      121 tgtagttagc atcactggtg attttctctt catattagga ttgagactga caggacaagt
      181 gacatccggt aattgtattt gcggagccaa cacttttccg tccactcctc ccgaagcgaa
      241 cccgtaccct gttaccctga ctctaaatgt aaattcatgt tgttggtacg gggtacgcct
      301 acttgtaagc tacggtatcc agttgggtgt gtagtgctcg tagtcattgt ctgtgcacat
      361 ttcaagtggt ccgtagcggg gccgggccag gccagcacga caaaaaagac cgtttggccg
      421 ccaagccgtg cgcggtccag cgcagtctag cactagagga gtaacgaaaa ataccattct
      481 catcccggca cgaacgcgag ggaacaacac aatcctgttt ttaccaaagt ccttttcccg
      541 aaacgaaaca caggttcgct ctgtaacacc agtaactctg cttacaagtt caccaatgcg
      601 agaccaccag gatacataga ttaccacaac tccgagccct tccacccaca gaatcagggg
      661 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
      721 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
      781 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
      841 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
      901 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
      961 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     1021 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     1081 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     1141 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     1201 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     1261 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     1321 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     1381 gttaagggat tttggtcatg cattctaggt gattattatt tgccgactac cttggtgatc
     1441 tcgcctttca cgtagtggac aaattcttcc aactgatctg cgcgcgaggc caagcgatct
     1501 tcttcttgtc caagataagc ctgtctagct tcaagtatga cgggctgata ctgggccggc
     1561 aggcgctcca ttgcccagtc ggcagcgaca tccttcggcg cgattttgcc ggttactgcg
     1621 ctgtaccaaa tgcgggacaa cgtaagcact acatttcgct catcgccagc ccagtcgggc
     1681 ggcgagttcc atagcgttaa ggtttcattt agcgcctcaa atagatcctg ttcaggaacc
     1741 ggatcaaaga gttcctccgc cgctggacct accaaggcaa cgctatgttc tcttgctttt
     1801 gtcagcaaga tagccagatc aatgtcgatc gtggctggct cgaagatacc agcaagaatg
     1861 tcattgcgct gccattctcc aaattgcagt tcgcgcttag ctggataacg ccacggaatg
     1921 atgtcgtcgt gcacaacaat ggtgacttct acagcgcgga gaatctcact ctctccaggg
     1981 gaagccgaag tttccaaaag gtcgttgatc aaagctcgcc gcgttgtttc atcaagcctt
     2041 acggtcaccg taaccagcaa atcaatatca ctgtgtggct tcaggccgcc atccactgcg
     2101 gagccgtaca aatgtacggc cagcaacgtc ggttcgagat ggcgctcgat gacgccaact
     2161 acctctgata gttgagtcga tacttcggcg atcaccgctt ccctcatgcg aaacgatcct
     2221 catcctgtct cttgatcaga tattgatccc ctgcgccatc agatccttgg cggcaagaaa
     2281 gccatccagt ttactttgca gggcttccca accttaccag agggcgcccc agctggcaat
     2341 tccggttcgc ttgctaagct tgcatgcctg caagtcgact ctagaggagc ggtgatcaca
     2401 ggcagcaacg ctctgtcatc gttacaatca acatgctacc ctccgcgaga tcatccgtgt
     2461 ttcaaacccg gcagcttagt tgccgttctt ccgaatagca tcggtaacat gagcaaagtc
     2521 tgccgcctta caacggctct cccgctgacg ccgtcccgga ctgatgggct gcctgtatcg
     2581 agtggtgatt ttgtgccgag ct
//
