LOCUS       sequence-298018-        2541 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Diatom terminator for P. tricornutum; uLoop specific syntax; EF 
            overhangs; Lvl 0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Windhagauer , M., Abbriano, RM., Ashworth, J., Barolo, L., 
            Jaramillo-Madrid, A.C., Pernice, M., Doblin, M.A.
  TITLE     Characterisation of novel regulatory sequences compatible with 
            modular assembly in the diatom Phaeodactylum tricornutum
  JOURNAL   Algal Research , Volume 53, March 2021
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Algal 
            Research , Volume 53, March 2021"
COMMENT     Sequence Label: pL0_EF_tNub
FEATURES             Location/Qualifiers
     source          1..2541
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(676..1264)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(756..775)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1355..2146)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cattactcgc atccattctc aggctgtctc gtctcgtctc aggtctccgc ttgcagagtg
       61 agactatgaa tattttcaaa tggattgagg gaagctttat ggtacaacaa gagttcgctg
      121 ccagcaaagt caatcgaaca atcttacaga agaggtaaac atttgtagcc gagataagga
      181 tttggtttat tttactgata tgagaggcat atacagagat ttgcgggtag ttttcgctgc
      241 gtctcgttgc tcatttgcta aggccatcaa ggcactccgg agatcagctc ctcgcgagaa
      301 tagtgccgct gaagggtcgg gatgagattt gacatgcttc gctccttacg ggagcaagac
      361 attgaagagg gaaacactac acccgaaaca ggctcgttct tacagtggac aaccatctat
      421 tcaccggaat gtgtatagac tcttcacagc cactcaacct tttgacttgg aacacctcct
      481 tgaaagtgaa gctcattgga attcgaaatc ctgtcagaat cgtttaacct aggcgctcga
      541 gaccaccagg atacatagat taccacaact ccgagccctt ccacccacag aatcagggga
      601 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      661 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
      721 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      781 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      841 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
      901 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      961 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     1021 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     1081 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     1141 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     1201 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     1261 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     1321 ttaagggatt ttggtcatgc attctaggtg attattattt gccgactacc ttggtgatct
     1381 cgcctttcac gtagtggaca aattcttcca actgatctgc gcgcgaggcc aagcgatctt
     1441 cttcttgtcc aagataagcc tgtctagctt caagtatgac gggctgatac tgggccggca
     1501 ggcgctccat tgcccagtcg gcagcgacat ccttcggcgc gattttgccg gttactgcgc
     1561 tgtaccaaat gcgggacaac gtaagcacta catttcgctc atcgccagcc cagtcgggcg
     1621 gcgagttcca tagcgttaag gtttcattta gcgcctcaaa tagatcctgt tcaggaaccg
     1681 gatcaaagag ttcctccgcc gctggaccta ccaaggcaac gctatgttct cttgcttttg
     1741 tcagcaagat agccagatca atgtcgatcg tggctggctc gaagatacca gcaagaatgt
     1801 cattgcgctg ccattctcca aattgcagtt cgcgcttagc tggataacgc cacggaatga
     1861 tgtcgtcgtg cacaacaatg gtgacttcta cagcgcggag aatctcactc tctccagggg
     1921 aagccgaagt ttccaaaagg tcgttgatca aagctcgccg cgttgtttca tcaagcctta
     1981 cggtcaccgt aaccagcaaa tcaatatcac tgtgtggctt caggccgcca tccactgcgg
     2041 agccgtacaa atgtacggcc agcaacgtcg gttcgagatg gcgctcgatg acgccaacta
     2101 cctctgatag ttgagtcgat acttcggcga tcaccgcttc cctcatgcga aacgatcctc
     2161 atcctgtctc ttgatcagat attgatcccc tgcgccatca gatccttggc ggcaagaaag
     2221 ccatccagtt tactttgcag ggcttcccaa ccttaccaga gggcgcccca gctggcaatt
     2281 ccggttcgct tgctaagctt gcatgcctgc aagtcgactc tagaggagcg gtgatcacag
     2341 gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc tccgcgagat catccgtgtt
     2401 tcaaacccgg cagcttagtt gccgttcttc cgaatagcat cggtaacatg agcaaagtct
     2461 gccgccttac aacggctctc ccgctgacgc cgtcccggac tgatgggctg cctgtatcga
     2521 gtggtgattt tgtgccgagc t
//
