LOCUS       sequence-29807-e         667 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Lindsten K, de Vrij FM, Verhoef LG, Fischer DF, van Leeuwen FW, Hol 
            EM, Masucci MG, Dantuma NP
  TITLE     Mutant ubiquitin found in neurodegenerative disorders is a ubiquitin
            fusion degradation substrate that blocks proteasomal degradation.
  JOURNAL   J Cell Biol. 2002 Apr 29. 157(3):417-27.
  PUBMED    11980917
REFERENCE   2  (bases 1 to 667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2002 Apr 29. 157(3):417-27."
COMMENT     Sequence Label: pcDNA3 Ub2-UBB+1
FEATURES             Location/Qualifiers
     source          1..667
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(84..100)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(85..101)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             114..338
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRG"
     CDS             348..572
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRG"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct tatcgatacc gtcgacctcg agggggggcc gccatgcaga
      121 tcttcgtgaa aacccttacc ggcaagacca tcacccttga ggtggagccc agtgacacca
      181 tcgaaaatgt gaaggccaag atccaggata aggaaggcat tccccccgac cagcagaggc
      241 tcatctttgc aggcaagcag ctggaagatg gccgtactct ttctgactac aacatccaga
      301 aggagtcgac cctgcacctg gtcctgcgtc tgagagggta tgctagaatg cagatcttcg
      361 tgaagactct gactggtaag accatcaccc tcgaggttga gcccagtgac accattgaga
      421 atgtcaaggc aaagatccaa gataaggaag gcatccctcc tgaccagcag aggctgatct
      481 ttgctggaaa acagctggaa gatgggcgca ccctgtctga ctacaacatc cagaaagagt
      541 ccaccctgca cctggtactc cgtctcagag gtgtggctag aatgcagatc ttcgtgaaga
      601 ctctgactgg taagaccatc accctcgagg ttgagcccag tgacaccatt gagaatgtca
      661 aggcaaa
//
