LOCUS       sequence-298071-        5907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast Expression plasmid for SARS-CoV-2 ORF7b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5907)
  TITLE     SARS-CoV-2 protein Yeast expression constructs, tagged, for protein 
            expression and/or purification
REFERENCE   2  (bases 1 to 5907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4046189
FEATURES             Location/Qualifiers
     source          1..5907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             502..519
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(1662..1680)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             2031..2834
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      2917..3899
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     terminator      3977..4006
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      4028..4615
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(4627..4654)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(4678..4869)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(4870..5661,5662..5730))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5493..5512
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5731..5833)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(5834..5865)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 gcggccgccc cacacaccat agcttcaaaa tgtttctact ccttttttac tcttccagat
       61 tttctcggac tccgcgcatc gccgtaccac ttcaaaacac ccaagcacag catactaaat
      121 ttcccctctt tcttcctcta gggtgtcgtt aattacccgt actaaaggtt tggaaaagaa
      181 aaaagagacc gcctcgtttc tttttcttcg gatttttttc tctttcgatg acctcccatt
      241 gatatttaag ttaataaacg gtcttcaatt tctcaagttt cagtttcatt tttcttgttc
      301 tattacaact ttttttactt cttgctcatt agaaagaaag catagcaatc taatctaagt
      361 tttaattaca aaatgatcga gttatccttg atagatttct atctatgttt tttggcattc
      421 ctgctttttc tcgtgttgat tatgttgatt atcttctggt tcagtttaga attgcaagac
      481 cataatgaaa catgccacgc cctcgtgccg agaggttcta aaatcgagga aggtaagcta
      541 gtcatttgga taaatggaga taagggttac aacggtttgg cagaagtagg caagaaattt
      601 gaaaaggaca caggtatcaa ggtgacggtt gaacatccag acaagttaga agaaaaattc
      661 cctcaagttg ctgctactgg tgatggtccc gacattattt tctgggccca cgatcgtttt
      721 ggtggatatg ctcagtcagg tttgctggct gagatcaccc cagacaaggc cttccaagat
      781 aaattgtacc cattcacttg ggatgctgtc agatacaacg gtaagcttat agcatatcca
      841 attgctgttg aagccttatc gttgatctac aataaggact tgttacctaa cccaccaaag
      901 acatgggaag aaattccagc tttggataaa gagctaaagg ccaagggaaa aagcgctttg
      961 atgtttaact tacaagaacc ttatttcacc tggccattaa tcgctgctga cggtggttac
     1021 gcatttaaat acgaaaacgg taagtatgac attaaggatg tcggcgttga taatgctggt
     1081 gcaaaggctg gtttgacctt cttggttgat ttaataaaga ataaacacat gaacgctgac
     1141 actgactact ctatcgccga agccgctttc aacaagggtg aaactgctat gaccataaat
     1201 ggtccatggg cctggagtaa cattgatact tctaaagtaa actatggtgt cactgttttg
     1261 cctacattca aaggccaacc atccaagccc tttgtcggtg ttctatccgc tggcatcaat
     1321 gctgcttctc caaacaagga gctagctaaa gaatttttgg aaaattactt gttaactgac
     1381 gaaggtttgg aggccgttaa caaggacaag ccattgggtg ctgtcgcttt gaaatcttac
     1441 gaagaagaat tggctaagga tcccagaatt gccgctacca tggagaacgc ccaaaagggt
     1501 gaaattatgc caaacatccc acaaatgtca gccttctggt acgcagttag gactgccgtg
     1561 attaatgctg cttccggtag acaaaccgtc gacgaagcat taaaagatgc tcaaacgtaa
     1621 acaggcccct tttcctttgt cgatatcatg taattagtta tgtcacgctt acattcacgc
     1681 cctcctccca catccgctct aaccgaaaag gaaggagtta gacaacctga agtctaggtc
     1741 cctatttatt ttttttaata gttatgttag tattaagaac gttatttata tttcaaattt
     1801 ttcttttttt tctgtacaaa gcggccgccg aaccactagt gcactgcagt acacggtttc
     1861 cttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca
     1921 gacttagatt ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat
     1981 tcttaaccca actgcacaga acaaaaacgt gcaggaaacg aagataaatc atgtcgaaag
     2041 ctacatataa ggaacgtgct gctactcatc ctagtcctgt tgctgccaag ctatttaata
     2101 tcatgcacga aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc accaaggaat
     2161 tactggagtt agttgaagca ttaggtccca aaatttgttt actaaaaaca catgtggata
     2221 tcttgactga tttttccatg gagggcacag ttaagccgct aaaggcatta tccgccaagt
     2281 acaatttttt actcttcgag gacagaaaat ttgctgacat tggtaataca gtcaaattgc
     2341 agtactctgc gggtgtatac agaatagcag aatgggcaga cattacgaat gcacacggtg
     2401 tggtgggccc aggtattgtt agcggtttga agcaggcggc agaagaagta acaaaggaac
     2461 ctagaggcct tttgatgtta gcagaattgt catgcaaggg ctccctatct actggagaat
     2521 atactaaggg tactgttgac attgcgaaga gcgacaaaga ttttgttatc ggctttattg
     2581 ctcaaagaga catgggtgga agagatgaag gttacgattg gttgattatg acacccggtg
     2641 tgggtttaga tgacaaggga gatgcattgg gtcaacagta tagaaccgtg gatgatgtgg
     2701 tttctacagg atctgacatt attattgttg gaagaggact atttgcaaag ggaagggatg
     2761 ctaaggtaga gggtgaacgt tacagaaaag caggctggga agcatatttg agaagatgcg
     2821 gccagcaaaa ctaaaaaact gtattataag taaatgcatg tatactaaac tcacaaatta
     2881 gagcttcaat ttaattatat cagttattac ccgagtaacg aagcatctgt gcttcatttt
     2941 gtagaacaaa aatgcaacgc gagagcgcta atttttcaaa caaagaatct gagctgcatt
     3001 tttacagaac agaaatgcaa cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca
     3061 tttttgtaaa acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag aatctgagct
     3121 gcatttttac agaacagaaa tgcaacgcga gagcgctatt ttaccaacaa agaatctata
     3181 cttctttttt gttctacaaa aatgcatccc gagagcgcta tttttctaac aaagcatctt
     3241 agattacttt ttttctcctt tgtgcgctct ataatgcagt ctcttgataa ctttttgcac
     3301 tgtaggtccg ttaaggttag aagaaggcta ctttggtgtc tattttctct tccataaaaa
     3361 aagcctgact ccacttcccg cgtttactga ttactagcga agctgcgggt gcattttttc
     3421 aagataaagg catccccgat tatattctat accgatgtgg attgcgcata ctttgtgaac
     3481 agaaagtgat agcgttgatg attcttcatt ggtcagaaaa ttatgaacgg tttcttctat
     3541 tttgtctcta tatactacgt ataggaaatg tttacatttt cgtattgttt tcgattcact
     3601 ctatgaatag ttcttactac aatttttttg tctaaagagt aatactagag ataaacataa
     3661 aaaatgtaga ggtcgagttt agatgcaagt tcaaggagcg aaaggtggat gggtaggtta
     3721 tatagggata tagcacagag atatatagca aagagatact tttgagcaat gtttgtggaa
     3781 gcggtattcg caatatttta gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa
     3841 gtgcgtcatc agagcgcttt tggttttcaa aagcgctctg aagttcctat actttctagc
     3901 tagagaatag gaacttcccg agcggccgcg tgttacaagc ttgactccag cgtaactgga
     3961 ctgcaatcaa ctcactggct caccttcacg ggtgggcctt tcttcggtag aaaatcaaag
     4021 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     4081 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaggtaac
     4141 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     4201 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     4261 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     4321 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     4381 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     4441 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     4501 gagggagctt ccaggggtaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     4561 ctgacttgag catcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     4621 cagcaacgca gaaaggccca cccgaaggtg agccaggtga ttacatttgg gccctcatca
     4681 gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg
     4741 gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc
     4801 cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg
     4861 tttggtcact taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     4921 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatgcggg agggcttacc
     4981 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     5041 agcaataaac cagccagccg ggagggccga gcgcagaagt gatcctgcaa ctttatccgc
     5101 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     5161 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     5221 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     5281 caaaaaagcg gttagctcct tcggtcctcc gatcgttgcc agaagtaagt tggccgcagt
     5341 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtgag
     5401 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     5461 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     5521 aaaagtgctc atcattggaa aacgttcttc ggggcgtaaa ctctcaagga tcttaccgct
     5581 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     5641 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     5701 aagggcgaca cggaaatgtt gaatactcat tttagcttcc ttagctcctg aaaatctcga
     5761 taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct
     5821 tacgtgccga tcaagtcaaa agcctccggt cggaggcttt tgactttctg ctatggaggt
     5881 caggtatgat ttaaatggtc agttaga
//
