LOCUS       sequence-29809-e         642 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Beskow A, Grimberg KB, Bott LC, Salomons FA, Dantuma NP, Young P
  TITLE     A conserved unfoldase activity for the p97 AAA-ATPase in proteasomal
            degradation.
  JOURNAL   J Mol Biol. 2009 Dec 11. 394(4):732-46.
  PUBMED    19782090
REFERENCE   2  (bases 1 to 642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2009 Dec 11. 394(4):732-46."
COMMENT     Sequence Label: UbG76V-eGFP-V5His_15aa
FEATURES             Location/Qualifiers
     source          1..642
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             63..287
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRG"
     regulatory      324..333
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(375..396)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 agctggttta gtgaccgtca gatccgctag cgctaccgga ctcagatctc gagctcggat
       61 ccatgcagat cttcgtgaag actctgactg gtaagaccat caccctcgag gttgagccca
      121 gtgacaccat tgagaatgtc aaggcaaaga tccaagataa ggaaggcatc cctcctgacc
      181 agcagaggct gatctttgct ggaaaacagc tggaagatgg gcgcaccctg tctgactaca
      241 acatccagaa agagtccacc ctgcacctgg tactccgtct cagaggtgtg gtggggaagc
      301 ttggtcgaca ggatccaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg
      361 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
      421 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
      481 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
      541 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
      601 aaggctacgt ccaggagcgc accatcttct tcaaggacga cg
//
