LOCUS       sequence-298095-        9140 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9140)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 9140)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3014";
            journalName: "Nat Struct Mol Biol"; date: "2015-05"; volume: "22"; 
            issue: "5"; pages: "362-9"
COMMENT     Sequence Label: CD97-Tango
FEATURES             Location/Qualifiers
     source          1..9140
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(3464..4078,4079..4465)
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the tetracycline repressor TetR with
                     the C-terminal activation domain of herpes simplex virus 
                     VP16"
                     /label=tTA
                     /note="In the Tet-Off(R) system, tTA binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="RLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVKN
                     KRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRPT
                     EKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTDS
                     MPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTIEG
                     LLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHADA
                     LDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(3531..3550)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(4497..4514)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4503..4727
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4773..5201
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4860..4879)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5070..5091
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5210..5230)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5215..5544
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5395..5530
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5457..5476
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5611..6405
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5665..5684)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6275..6294
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6579..6700
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6616..6635)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6670..6689
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6749..6765)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6749..6765)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6762..6784)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6773..6789
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6797..6803,6804..6821,6822..6827))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6842..6863
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6980..6997)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7151..7739)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7231..7250)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7910..8701,8702..8770))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8533..8552
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8771..8875)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8950..8969)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacttaagc ttggtaccga gctcggatcc actagtccag tgtggtggaa
      721 ttctgcagat atccagcaca gtggcggccg cgccaccatg aagacgatca tcgccctgag
      781 ctacatcttc tgcctggtat tcgccgacta caaggacgat gatgacgcca gcatcgatat
      841 gggggggcgg gtgttcctgg ccttttgtgt ttggctgacg ctccccggag cggagacaca
      901 ggattcaagg gggtgtgcac ggtggtgtcc tcagaactct agctgcgtga acgccacagc
      961 atgccggtgt aatccaggct ttagtagctt ctctgagatt atcaccaccc ctactgagac
     1021 ttgcgacgat attaacgagt gtgcaacccc tagcaaagtg tcatgcggga agttttctga
     1081 ctgctggaac acagagggca gctatgactg cgtctgtagc ccaggctatg agccagtgtc
     1141 tggagctaag accttcaaga atgagagtga gaacacttgc caggatgtgg acgagtgcca
     1201 acagaaccca cgactctgta agagttatgg gacgtgtgta aacaccctgg gctcatacac
     1261 ttgccaatgc cttccgggat ttaaattcat tccagaggat cccaaagtgt gtacagacgt
     1321 caatgagtgc acatccggac agaacccttg ccattccagc acgcactgtc tgaataatgt
     1381 cgggtcttac cagtgccggt gtagacctgg ctggcagccg atccccggct ccccgaatgg
     1441 acccaataac acagtttgtg aagatgtcga tgagtgttcc tctggacagc accaatgcga
     1501 ttcatctacc gtctgtttta acaccgtcgg atcttacagt tgccgctgcc gccccggttg
     1561 gaaacctcgg catgggattc caaataacca gaaagacaca gtgtgcgaag acatgacatt
     1621 cagcacatgg actcctcccc cgggcgtcca tagtcaaacc cttagcagat tctttgacaa
     1681 agtccaggat ctgggaagag atagcaaaac aagctccgcc gaggtgacta tccagaacgt
     1741 tatcaagctg gtagatgagc tgatggaggc cccgggcgac gtcgaggcgc tcgctccccc
     1801 ggtccggcat ctgattgcca cacagctttt gagcaatctg gaggatatta tgcgcatact
     1861 ggctaaaagc ctgccaaagg gcccgttcac gtatattagc ccctccaaca cagagcttac
     1921 gctcatgatc caggaacgag gggataagaa tgtgacaatg ggccagtcct ccgcgaggat
     1981 gaagctcaac tgggcagttg ctgccggagc cgaagaccct ggccctgccg tcgctggaat
     2041 actgagtata cagaatatga ctacactgct tgccaacgct agcctgaacc tgcattccaa
     2101 aaagcaagcg gaactcgaag agatttacga aagctccatt agaggcgtgc aacttcgcag
     2161 gctgagtgca gtgaactcta tattcctgtc tcacaacaat accaaagagc tgaattcccc
     2221 tatactcttc gccttcagcc atctggaatc atccgacgga gaggccgggc gggatccccc
     2281 agcaaaggac gtgatgcctg gtcctagaca ggaactcttg tgtgccttct ggaagtccga
     2341 ctctgacagg ggaggccact gggctaccga gggctgccaa gtgctgggct ccaagaacgg
     2401 gagtactaca tgtcagtgta gtcacctctc tagtttcgcc atccttatgg ctcactacga
     2461 tgtagaagat tggaaattga cactgatcac gagggtggga ctcgccttgt ccctgttctg
     2521 cctgctcctg tgcatcttga cattcctcct cgttcgcccc atacaaggat ctaggaccac
     2581 aatccacctg catttgtgta tctgcctgtt cgttggaagc accatcttcc ttgccggcat
     2641 tgaaaatgag gggggacaag tgggactgcg gtgtagactg gtggctggtc tcctgcacta
     2701 ctgcttcctg gctgcgttct gctggatgtc tctggagggc ctcgaactgt acttccttgt
     2761 tgttagagtg ttccagggcc agggactctc tacacggtgg ctctgcctga tcggctacgg
     2821 cgtgcccctt ctgatcgtcg gtgtgagcgc cgcgatctac tcaaaaggat acggccgccc
     2881 ccgctattgt tggctggact tcgaacaggg ctttctctgg tcctttctgg gtcccgtgac
     2941 attcattatc ttgtgcaacg cagttatctt tgtcaccacc gtgtggaaat tgacacaaaa
     3001 attctccgag atcaatcccg atatgaagaa gctgaagaag gccagagctc ttaccatcac
     3061 agcaattgcg cagctgtttc tgctggggtg tacctgggtc tttggactct tcatatttga
     3121 tgacagaagt ttggtgctga cttatgtgtt tactattctg aactgccttc agggtgcctt
     3181 cctctatctt ctccattgcc tgctgaacaa gaaagtccgc gaggaatatc gaaagtgggc
     3241 ctgtctggtc gccgggggga gtaagtactc tgagtttacc tccactacat ctggaacggg
     3301 ccacaatcag actagagctc tccgggccag cgagagtgga atcatcgata ccggtggacg
     3361 caccccaccc agcctgggtc cccaagatga gtcctgcacc accgccagct cctccctggc
     3421 caaggacact tcatcgaccg gtgagaacct gtacttccag ctaagattag ataaaagtaa
     3481 agtgattaac agcgcattag agctgcttaa tgaggtcgga atcgaaggtt taacaacccg
     3541 taaactcgcc cagaagctag gtgtagagca gcctacattg tattggcatg taaaaaataa
     3601 gcgggctttg ctcgacgcct tagccattga gatgttagat aggcaccata ctcacttttg
     3661 ccctttagaa ggggaaagct ggcaagattt tttacgtaat aacgctaaaa gttttagatg
     3721 tgctttacta agtcatcgcg atggagcaaa agtacattta ggtacacggc ctacagaaaa
     3781 acagtatgaa actctcgaaa atcaattagc ctttttatgc caacaaggtt tttcactaga
     3841 gaatgcatta tatgcactca gcgctgtggg gcattttact ttaggttgcg tattggaaga
     3901 tcaagagcat caagtcgcta aagaagaaag ggaaacacct actactgata gtatgccgcc
     3961 attattacga caagctatcg aattatttga tcaccaaggt gcagagccag ccttcttatt
     4021 cggccttgaa ttgatcatat gcggattaga aaaacaactt aaatgtgaaa gtgggtccgc
     4081 gtacagccgc gcgcgtacga aaaacaatta cgggtctacc atcgagggcc tgctcgatct
     4141 cccggacgac gacgcccccg aagaggcggg gctggcggct ccgcgcctgt cctttctccc
     4201 cgcgggacac acgcgcagac tgtcgacggc ccccccgacc gatgtcagcc tgggggacga
     4261 gctccactta gacggcgagg acgtggcgat ggcgcatgcc gacgcgctag acgatttcga
     4321 tctggacatg ttgggggacg gggattcccc gggtccggga tttacccccc acgactccgc
     4381 cccctacggc gctctggata tggccgactt cgagtttgag cagatgttta ccgatgccct
     4441 tggaattgac gagtacggtg ggtagtctag agggcccgtt taaacccgct gatcagcctc
     4501 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     4561 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     4621 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     4681 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     4741 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     4801 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     4861 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     4921 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     4981 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     5041 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     5101 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     5161 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     5221 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     5281 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     5341 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     5401 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     5461 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     5521 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     5581 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     5641 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     5701 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     5761 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
     5821 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     5881 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     5941 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     6001 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     6061 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     6121 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     6181 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     6241 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     6301 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     6361 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     6421 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
     6481 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     6541 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     6601 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     6661 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     6721 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     6781 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     6841 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     6901 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     6961 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     7021 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     7081 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     7141 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     7201 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     7261 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     7321 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     7381 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     7441 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     7501 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     7561 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     7621 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     7681 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     7741 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     7801 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     7861 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     7921 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     7981 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     8041 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     8101 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     8161 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     8221 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     8281 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     8341 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     8401 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     8461 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     8521 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     8581 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     8641 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     8701 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     8761 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     8821 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     8881 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg
     8941 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg
     9001 tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt
     9061 gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt
     9121 acgggccaga tatacgcgtt
//
