LOCUS       sequence-298097-       11198 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible expression of Human NFKB1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11198)
  AUTHORS   Rosa FF, Pires CF, Kurochkin I, Ferreira AG, Gomes AM, Palma LG, 
            Shaiv K, Solanas L, Azenha C, Papatsenko D, Schulz O, Reis e Sousa 
            C, Pereira CF
  TITLE     Direct reprogramming of fibroblasts into antigen-presenting 
            dendritic cells.
  JOURNAL   Sci Immunol. 2018 Dec 7;3(30). pii: 3/30/eaau4292. doi: 
            10.1126/sciimmunol.aau4292.
  PUBMED    30530727
REFERENCE   2  (bases 1 to 11198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2018-12-7"; volume: "3(30). pii: 3/30/eaau4292. 
            doi"; pages: " 10.1126/sciimmunol.aau4292"
COMMENT     Sequence Label: pFUW-tetO-NFKB1
FEATURES             Location/Qualifiers
     source          1..11198
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    167..284
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(341..359,360..382,383..401,402..424,425..443,444..466,
                     467..485,486..508,509..527,528..550,551..569,570..592,
                     593..611)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     misc_feature    3617..4205
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3670..3690)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4088..4099)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4208..4224)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4209..4225)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4730..4910
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4936..4953)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4942..5166
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5212..5640
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5299..5318)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5509..5530
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5649..5669)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5654..5983
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5834..5969
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5896..5915
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6031..6078
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6097..6471
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6601..6722
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6638..6657)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6692..6711
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6771..6787)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6771..6787)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6784..6806)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6795..6811
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6819..6825,6826..6843,6844..6849))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6864..6885
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7002..7019)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7173..7761)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7253..7272)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7932..8723,8724..8792))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8555..8574
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8793..8897)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8972..8991)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9163..9542
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9544..9742
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9697..9717
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9760..9940
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9987..10112
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10605..10838
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11023..11067
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
       61 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatcggc
      121 actgcgtgcg ccaattctgc agacaaatgg cagtattcat ccacaatttt aaaagaaaag
      181 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
      241 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
      301 acagcagaga tccagtttgg ttagatctcg agtttaccac tccctatcag tgatagagaa
      361 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
      421 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
      481 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
      541 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
      601 agtgatagag aaamgysawm swcgmkstyk kkaccyssrt mgaagwcacc gggaccgatc
      661 cagcctccgc ggccccgaat tcatggcaga agatgatcca tatttgggaa ggcctgaaca
      721 aatgtttcat ttggatcctt ctttgactca tacaatattt aatccagaag tatttcaacc
      781 acagatggca ctgccaacag cagatggccc ataccttcaa atattagagc aacctaaaca
      841 gagaggattt cgtttccgtt atgtatgtga aggcccatcc catggtggac tacctggtgc
      901 ctctagtgaa aagaacaaga agtcttaccc tcaggtcaaa atctgcaact atgtgggacc
      961 agcaaaggtt attgttcagt tggtcacaaa tggaaaaaat atccacctgc atgcccacag
     1021 cctggtggga aaacactgtg aggatgggat ctgcactgta actgctggac ccaaggacat
     1081 ggtggtcggc ttcgcaaacc tgggtatact tcatgtgaca aagaaaaaag tatttgaaac
     1141 actggaagca cgaatgacag aggcgtgtat aaggggctat aatcctggac tcttggtgca
     1201 ccctgacctt gcctatttgc aagcagaagg tggaggggac cggcagctgg gagatcggga
     1261 aaaagagcta atccgccaag cagctctgca gcagaccaag gagatggacc tcagcgtggt
     1321 gcggctcatg tttacagctt ttcttccgga tagcactggc agcttcacaa ggcgcctgga
     1381 acccgtggta tcagacgcca tctatgacag taaagccccc aatgcatcca acttgaaaat
     1441 tgtaagaatg gacaggacag ctggatgtgt gactggaggg gaggaaattt atcttctttg
     1501 tgacaaagtt cagaaagatg acatccagat tcgattttat gaagaggaag aaaatggtgg
     1561 agtctgggaa ggatttggag atttttcccc cacagatgtt catagacaat ttgccattgt
     1621 cttcaaaact ccaaagtata aagatattaa tattacaaaa ccagcctctg tgtttgtcca
     1681 gcttcggagg aaatctgact tggaaactag tgaaccaaaa cctttcctct actatcctga
     1741 aatcaaagat aaagaagaag tgcagaggaa acgtcagaag ctcatgccca atttttcgga
     1801 tagtttcggc ggtggtagtg gtgccggagc tggaggcgga ggcatgtttg gtagtggcgg
     1861 tggaggaggg ggcactggaa gtacaggtcc agggtatagc ttcccacact atggatttcc
     1921 tacttatggt gggattactt tccatcctgg aactactaaa tctaatgctg ggatgaagca
     1981 tggaaccatg gacactgaat ctaaaaagga ccctgaaggt tgtgacaaaa gtgatgacaa
     2041 aaacactgta aacctctttg ggaaagttat tgaaaccaca gagcaagatc aggagcccag
     2101 cgaggccacc gttgggaatg gtgaggtcac tctaacgtat gcaacaggaa caaaagaaga
     2161 gagtgctgga gttcaggata acctctttct agagaaggct atgcagcttg caaagaggca
     2221 tgccaatgcc cttttcgact acgcggtgac aggagacgtg aagatgctgc tggccgtcca
     2281 gcgccatctc actgctgtgc aggatgagaa tggggacagt gtcttacact tagcaatcat
     2341 ccaccttcat tctcaacttg tgagggatct actagaagtc acatctggtt tgatttctga
     2401 tgacattatc aacatgagaa atgatctgta ccagacgccc ttgcacttgg cagtgatcac
     2461 taagcaggaa gatgtggtgg aggatttgct gagggctggg gccgacctga gccttctgga
     2521 ccgcttgggt aactctgttt tgcacctagc tgccaaagaa ggacatgata aagttctcag
     2581 tatcttactc aagcacaaaa aggcagcact acttcttgac caccccaacg gggacggtct
     2641 gaatgccatt catctagcca tgatgagcaa tagcctgcca tgtttgctgc tgctggtggc
     2701 cgctggggct gacgtcaatg ctcaggagca gaagtccggg cgcacagcac tgcacctggc
     2761 tgtggagcac gacaacatct cattggcagg ctgcctgctc ctggagggtg atgcccatgt
     2821 ggacagtact acctacgatg gaaccacacc cctgcatata gcagctggga gagggtccac
     2881 caggctggca gctcttctca aagcagcagg agcagatccc ctggtggaga actttgagcc
     2941 tctctatgac ctggatgact cttgggaaaa tgcaggagag gatgaaggag ttgtgcctgg
     3001 aaccacgcct ctagatatgg ccaccagctg gcaggtattt gacatattaa atgggaaacc
     3061 atatgagcca gagtttacat ctgatgattt actagcacaa ggagacatga aacagctggc
     3121 tgaagatgtg aagctgcagc tgtataagtt actagaaatt cctgatccag acaaaaactg
     3181 ggctactctg gcgcagaaat taggtctggg gatacttaat aatgccttcc ggctgagtcc
     3241 tgctccttcc aaaacactta tggacaacta tgaggtctct gggggtacag tcagagagct
     3301 ggtggaggcc ctgagacaaa tgggctacac cgaagcaatt gaagtgatcc aggcagcctc
     3361 cagcccagtg aagaccacct ctcaggccca ctcgctgcct ctctcgcctg cctccacaag
     3421 gcagcaaata gacgagctcc gagacagtga cagtgtctgc gacagcggcg tggagacatc
     3481 cttccgcaaa ctcagcttta ccgagtctct gaccagtggt gcctcactgc taactctcaa
     3541 caaaatgccc catgattatg ggcaggaagg acctctagaa ggcaaaattt aggaattcga
     3601 tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     3661 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     3721 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     3781 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     3841 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     3901 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     3961 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     4021 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     4081 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     4141 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     4201 cccgcatcga taccgtcgac ctcgagacct agaaaaacat ggagcaatca caagtagcaa
     4261 tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg
     4321 ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct
     4381 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     4441 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta
     4501 cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc
     4561 agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc
     4621 tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag
     4681 ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg
     4741 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     4801 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     4861 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gggcccgttt
     4921 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     4981 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     5041 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     5101 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     5161 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
     5221 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     5281 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     5341 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     5401 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     5461 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     5521 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     5581 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     5641 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     5701 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     5761 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     5821 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     5881 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     5941 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
     6001 tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc
     6061 ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt
     6121 ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg
     6181 ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg
     6241 ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg
     6301 cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac
     6361 gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg
     6421 cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacgtgcta
     6481 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     6541 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     6601 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     6661 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     6721 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     6781 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     6841 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     6901 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     6961 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     7021 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     7081 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     7141 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     7201 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     7261 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     7321 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     7381 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     7441 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     7501 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     7561 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     7621 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     7681 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     7741 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     7801 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     7861 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     7921 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     7981 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     8041 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     8101 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     8161 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     8221 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     8281 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     8341 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     8401 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     8461 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     8521 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     8581 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     8641 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     8701 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     8761 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     8821 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     8881 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga
     8941 gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     9001 gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt
     9061 aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc
     9121 gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta
     9181 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     9241 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     9301 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     9361 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     9421 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     9481 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     9541 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
     9601 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
     9661 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
     9721 ggtgggaggt ctatataagc agcgcgtttt gcctgtactg ggtctctctg gttagaccag
     9781 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     9841 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     9901 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
     9961 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
    10021 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
    10081 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
    10141 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
    10201 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
    10261 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
    10321 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
    10381 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
    10441 cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
    10501 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
    10561 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
    10621 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
    10681 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
    10741 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
    10801 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
    10861 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
    10921 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
    10981 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
    11041 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
    11101 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
    11161 gtactttcta tagtgaatag agttaggcag ggatattc
//
