LOCUS       sequence-29810-e         763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Beskow A, Grimberg KB, Bott LC, Salomons FA, Dantuma NP, Young P
  TITLE     A conserved unfoldase activity for the p97 AAA-ATPase in proteasomal
            degradation.
  JOURNAL   J Mol Biol. 2009 Dec 11. 394(4):732-46.
  PUBMED    19782090
REFERENCE   2  (bases 1 to 763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2009 Dec 11. 394(4):732-46."
COMMENT     Sequence Label: UbG76V-eGFP-V5His_15aa
FEATURES             Location/Qualifiers
     source          1..763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      21..30
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(67..291)
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRG"
     promoter        complement(351..554)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(380..400)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 acgctcctcg cccttgctca ccatggtggc gaccggtgga tcctgtcgac caagcttccc
       61 caccacacct ctgagacgga gtaccaggtg cagggtggac tctttctgga tgttgtagtc
      121 agacagggtg cgcccatctt ccagctgttt tccagcaaag atcagcctct gctggtcagg
      181 agggatgcct tccttatctt ggatctttgc cttgacattc tcaatggtgt cactgggctc
      241 aacctcgagg gtgatggtct taccagtcag agtcttcacg aagatctgca tggatccgag
      301 ctcgagatct gagtccggta gcgctagcgg atctgacggt tcactaaacc agctctgctt
      361 atatagacct cccaccgtac acgcctaccg cccatttgcg tcaatggggc ggagttgtta
      421 cgacattttg gaaagtcccg ttgattttgg tgccaaaaca aactcccatt gacgtcaatg
      481 gggtggagac ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt gatgtactgc
      541 caaaaccgca tcaccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga
      601 aagtcccata aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg
      661 tcaatagggg gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac
      721 cgtantactc cacccattga cgtcaatggn aanntcccta ttg
//
