LOCUS       sequence-298109-       11721 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast Expression plasmid for SARS-CoV-2 nsp3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11721)
  TITLE     SARS-CoV-2 protein Yeast expression constructs, tagged, for protein 
            expression and/or purification
REFERENCE   2  (bases 1 to 11721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4046323
FEATURES             Location/Qualifiers
     source          1..11721
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        25..466
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    25..142
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     437..460
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             6316..6333
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(7476..7494)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             7845..8648
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      8731..9713
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     terminator      9791..9820
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      9842..10429
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(10441..10468)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(10492..10683)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(10684..11475,11476..11544))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11307..11326
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11545..11647)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(11648..11679)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 gcggccgctt gctatattga agtacggatt agaagccgcc gagcgggcga cagccctccg
       61 acggaagact ctcctccgtg cgtcctcgtc ttcaccggtc gcgttcctga aacgcagatg
      121 tgcctcgcgc cgcactgctc cgaacaataa agattctaca atactagctt ttatggttat
      181 gaagaggaaa aattggcagt aacctggccc cacaaacctt caaattaacg aatcaaatta
      241 acaaccatag gatgataatg cgattagttt tttagcctta tttctggggt aattaatcag
      301 cgaagcgatg atttttgatc tattaacaga tatataaatg gaaaagctgc ataaccactt
      361 taactaatac tttcaacatt ttcagtttgt attacttctt attcaaatgt cataaaagta
      421 tcaacaaaaa attgttaata tacctctata ctttaacgtc aaggagaaaa aactataatg
      481 gcccccacta aggtcacatt cggggatgac accgtgatag aggttcaggg ttacaaatcc
      541 gttaacatca cttttgaatt agatgaaaga attgacaagg tattgaatga aaagtgctca
      601 gcatataccg ttgaactggg tacggaggtc aacgaatttg cttgtgtcgt tgcggatgct
      661 gttatcaaaa ctttgcaacc tgtgtctgaa cttctaaccc cattaggaat tgacttggat
      721 gaatggtcta tggctactta ctatctattc gatgaaagtg gtgagttcaa gttggcttcc
      781 cacatgtact gttcgttcta cccaccagac gaagacgaag aagaaggcga ctgtgaagaa
      841 gaagagtttg aaccaagcac ccaatatgaa tacggtacgg aagatgatta ccaaggtaag
      901 ccattggaat ttggtgccac ttctgctgca ttgcaacctg aagaagagca agaagaagat
      961 tggttagacg atgactctca gcaaacagtc ggtcaacaag acggttccga ggataatcaa
     1021 actaccacca ttcaaacaat cgttgaggta caaccacaac ttgaaatgga attgactcca
     1081 gtcgttcaga ccatagaagt taactcgttc tcaggttact tgaagctaac tgataatgtt
     1141 tatattaaaa acgctgacat cgtcgaagaa gcaaagaaag ttaagcctac cgtcgtggtt
     1201 aacgccgcta acgtttattt gaagcatggt ggcggtgtag ctggtgcttt aaacaaagcc
     1261 actaataacg ctatgcaagt cgaatctgac gattacattg ctaccaatgg tccattgaag
     1321 gtgggaggct cttgcgtctt aagtggacac aacttggcga aacattgtct ccacgttgtt
     1381 ggtcccaatg ttaacaaggg tgaagacatt caactgttga agtccgccta cgaaaatttc
     1441 aaccaacatg aggttctctt ggcaccatta ctgagtgctg gtatcttcgg cgctgaccca
     1501 attcactctt taagggtctg tgtggatact gtaagaacaa acgtctactt ggccgttttc
     1561 gataaaaacc tttatgacaa gttagtctct tcctttttgg aaatgaagtc agaaaagcaa
     1621 gttgaacaga aaatcgcgga aatccctaag gaagaagtaa agccgtttat aaccgaatcg
     1681 aagccctctg ttgagcaaag aaagcaagat gacaaaaaga ttaaagcttg tgttgaggaa
     1741 gtaacaacta cattagagga aactaagttt ctcacggaaa atctattgtt gtacatagat
     1801 atcaatggta acttgcaccc agactccgct actttggtca gcgatatcga cattacattc
     1861 ttgaagaagg acgctccata tattgtgggt gacgttgtcc aagaaggtgt tttaactgct
     1921 gttgtcatcc caactaagaa ggcagggggt accacggaga tgctagctaa ggccttgcgt
     1981 aaagtcccga ctgataatta tattaccacc taccctggtc agggtctaaa cggttacacg
     2041 gttgaagaag ctaaaactgt tctaaaaaag tgtaagtctg ctttttacat attgccatca
     2101 attatttcca acgaaaagca agaaatattg ggtaccgtat cttggaattt gagagaaatg
     2161 ttagctcatg ctgaggaaac tagaaagttg atgcctgtct gcgttgaaac taaagctatc
     2221 gtcagcacga tccaaagaaa atacaagggt attaaaatcc aagaaggcgt tgtggactat
     2281 ggagcccgtt tctatttcta cacttcgaag accaccgtcg cttcgttgat caacaccttg
     2341 aacgatctca acgagacact tgttactatg ccactcggct atgtaactca cggtcttaac
     2401 ttggaagaag ctgcgagata catgcgctct ctgaaagttc ctgccacagt ctccgtatct
     2461 tcaccagacg ctgttacagc ctataatgga tacttgactt cttcatcaaa aaccccagaa
     2521 gaacatttca tcgaaactat ctctctagct ggtagttaca aggattggtc ttactccggt
     2581 caatccacac aactaggtat agagtttctg aagagaggtg acaagagtgt ttactacacc
     2641 agcaatccaa cgacctttca tttagatggt gaagtcatta cgttcgataa cttgaagact
     2701 ttgttgagtt tgcgggaagt ccgaactatc aaggttttca ctaccgttga taacattaac
     2761 ctccacaccc aagttgttga tatgtctatg acttatggtc aacagttcgg tccgacatat
     2821 ttggatggtg ctgacgttac taaaattaag ccacacaact cccacgaagg gaagacattc
     2881 tacgtgcttc caaatgacga cactttacga gtcgaagcct ttgagtacta ccataccacg
     2941 gacccatctt tccttggtag atacatgtcc gctctgaatc acactaaaaa gtggaaatac
     3001 ccacaagtta acggtttgac ttctattaag tgggccgata ataactgtta cttggctacc
     3061 gccttattga ctttgcaaca gatagaactc aagtttaatc ctcccgctct acaagatgca
     3121 tattatagag ctagagcagg cgaagcggct aatttctgtg ctttaatttt ggcttactgt
     3181 aacaagaccg taggtgaatt gggggatgtc agagagacta tgtcctatct tttccagcac
     3241 gcaaacttgg attcatgtaa gagagtgtta aatgtagtct gcaagacctg cggtcaacaa
     3301 caaactacct taaagggtgt tgaagccgtt atgtacatgg gtacactttc ttacgaacaa
     3361 tttaagaagg gtgtccaaat cccatgtact tgtggtaagc aagccactaa atacttggtc
     3421 cagcaagaaa gccctttcgt tatgatgagc gccccaccag ctcaatacga gctgaagcat
     3481 ggaactttca cctgtgcttc tgaatatacc ggtaactacc aatgcggtca ctacaagcat
     3541 attactagca aagaaacttt gtactgtatc gatggtgcct tgttgactaa gtcctcagaa
     3601 tataagggcc caattactga cgtgttttac aaagagaata gttacactac cacaattaag
     3661 ccagtaacat acaaactgga tggtgttgtg tgtaccgaga tcgaccctaa gttagataac
     3721 tattacaaga aggacaactc ttactttacc gaacaaccga tcgacctagt cccaaatcag
     3781 ccatatccaa atgcttcttt tgataacttc aagttcgttt gtgataacat aaaattcgcc
     3841 gacgatttaa accaattgac cggttacaaa aagccggctt caagagagtt gaaagtcact
     3901 ttcttcccag atttaaatgg cgatgtcgtt gctattgact ataagcacta cactccatct
     3961 ttcaagaagg gcgccaagtt gctacacaag ccaattgtct ggcacgttaa caacgctacc
     4021 aacaaggcta cgtataagcc taatacctgg tgtatcagat gtctatggtc caccaaacca
     4081 gttgaaacga gtaactcttt tgacgttctg aaatcagaag atgcccaagg gatggataac
     4141 ttagcatgtg aagacttgaa acctgtttca gaagaagttg tggaaaatcc aactattcag
     4201 aaggacgtgt tggaatgcaa cgtgaaaaca actgaagtag ttggtgatat aatcctgaag
     4261 ccagctaaca actcactgaa gattaccgaa gaagtcggtc acaccgactt gatggcagcc
     4321 tacgttgata actcttccct cacgatcaaa aagccaaatg aattgtctcg tgtactcggg
     4381 ctaaagaccc tagctaccca tggtctagcg gctgtcaact ccgtgccgtg ggacaccatc
     4441 gctaactacg ctaagccttt tttgaataag gtcgttagca ccacgacaaa tatagttact
     4501 agatgtttga acagagtgtg taccaattac atgccttatt tctttactct attgctgcaa
     4561 ttgtgcactt tcactcgctc tacaaactcc agaattaaag catctatgcc aaccaccatc
     4621 gccaagaaca cggttaagtc agtaggtaag ttctgcttag aggcaagctt taactatttg
     4681 aagtctccaa acttctctaa gttgatcaat attattatct ggtttttact tttgagcgtt
     4741 tgtttgggtt ccttgatcta ctcaacggct gctttgggtg tcttgatgtc taacttgggt
     4801 atgccgagtt actgtactgg ttatagagaa ggttatttga actcaacaaa cgttactatt
     4861 gctacgtact gcaccggttc cattccatgt tctgtttgtt tatccggttt agactcgttg
     4921 gacacttacc catctttaga aaccatccaa attaccattt cctctttcaa gtgggatttg
     4981 acagctttcg gtcttgtcgc tgaatggttt ttggcctata tattgtttac tcgtttcttc
     5041 tacgtcttag gcttggccgc tatcatgcag ttgtttttct cttattttgc agtccatttt
     5101 atctcaaatt cgtggttgat gtggttgatt attaatctcg ttcaaatggc tccaatttcg
     5161 gcaatggtta ggatgtacat tttctttgct tcattctatt atgtatggaa atcttacgtc
     5221 catgtggttg atggttgtaa ctcctcgacc tgtatgatgt gttacaagag aaatagagcg
     5281 actagggttg agtgcacaac tatcgttaac ggtgtcagac gttctttcta cgtatacgct
     5341 aacggtggta agggcttttg taaactccat aactggaatt gtgttaattg tgataccttt
     5401 tgcgctggtt ctacctttat tagtgatgaa gtagccagag acttaagtct ccaattcaaa
     5461 cggccaatca acccaactga tcaatcctct tatattgtcg atagtgtcac cgttaagaac
     5521 ggtagtatcc acttatactt cgataaggcc ggtcaaaaga catacgaaag acactcattg
     5581 tcccactttg ttaatttaga caatttgaga gctaataata ctaaggggtc tttacccatc
     5641 aacgtcatag tcttcgatgg taagtctaaa tgtgaagaat cctccgctaa gtctgcgtca
     5701 gtttactact ctcaattgat gtgccaaccc atcctacttc tggatcaagc tttggtatct
     5761 gacgttggtg actctgccga ggttgctgtt aagatgttcg atgcttacgt taacacattt
     5821 tcatcaactt tcaacgtgcc aatggaaaag ctgaagactt tggtggctac cgctgaagca
     5881 gaattggcta agaatgtttc tttggataac gttttaagta cgttcatttc cgcagctcgt
     5941 cagggtttcg tcgactctga cgtagaaacc aaagacgtcg tcgaatgttt gaagctctct
     6001 catcaatccg atattgaagt cactggtgac tcctgtaata actacatgct gacgtataac
     6061 aaagtggaaa acatgactcc tagagatttg ggagcgtgca tcgactgttc tgctagacac
     6121 ataaacgctc aagttgcaaa atcccataat attgctttga tctggaacgt taaagatttc
     6181 atgtcactat ccgagcaact taggaaacaa atccggtctg cggcaaagaa aaataacctt
     6241 ccattcaaat taacatgtgc aacaactaga caagttgtga acgttgttac tactaagatc
     6301 gctctgaagg gtggtctcgt gccgagaggt tctaaaatcg aggaaggtaa gctagtcatt
     6361 tggataaatg gagataaggg ttacaacggt ttggcagaag taggcaagaa atttgaaaag
     6421 gacacaggta tcaaggtgac ggttgaacat ccagacaagt tagaagaaaa attccctcaa
     6481 gttgctgcta ctggtgatgg tcccgacatt attttctggg cccacgatcg ttttggtgga
     6541 tatgctcagt caggtttgct ggctgagatc accccagaca aggccttcca agataaattg
     6601 tacccattca cttgggatgc tgtcagatac aacggtaagc ttatagcata tccaattgct
     6661 gttgaagcct tatcgttgat ctacaataag gacttgttac ctaacccacc aaagacatgg
     6721 gaagaaattc cagctttgga taaagagcta aaggccaagg gaaaaagcgc tttgatgttt
     6781 aacttacaag aaccttattt cacctggcca ttaatcgctg ctgacggtgg ttacgcattt
     6841 aaatacgaaa acggtaagta tgacattaag gatgtcggcg ttgataatgc tggtgcaaag
     6901 gctggtttga ccttcttggt tgatttaata aagaataaac acatgaacgc tgacactgac
     6961 tactctatcg ccgaagccgc tttcaacaag ggtgaaactg ctatgaccat aaatggtcca
     7021 tgggcctgga gtaacattga tacttctaaa gtaaactatg gtgtcactgt tttgcctaca
     7081 ttcaaaggcc aaccatccaa gccctttgtc ggtgttctat ccgctggcat caatgctgct
     7141 tctccaaaca aggagctagc taaagaattt ttggaaaatt acttgttaac tgacgaaggt
     7201 ttggaggccg ttaacaagga caagccattg ggtgctgtcg ctttgaaatc ttacgaagaa
     7261 gaattggcta aggatcccag aattgccgct accatggaga acgcccaaaa gggtgaaatt
     7321 atgccaaaca tcccacaaat gtcagccttc tggtacgcag ttaggactgc cgtgattaat
     7381 gctgcttccg gtagacaaac cgtcgacgaa gcattaaaag atgctcaaac gtaaacaggc
     7441 cccttttcct ttgtcgatat catgtaatta gttatgtcac gcttacattc acgccctcct
     7501 cccacatccg ctctaaccga aaaggaagga gttagacaac ctgaagtcta ggtccctatt
     7561 tatttttttt aatagttatg ttagtattaa gaacgttatt tatatttcaa atttttcttt
     7621 tttttctgta caaagcggcc gccgaaccac tagtgcactg cagtacacgg tttccttgaa
     7681 atttttttga ttcggtaatc tccgaacaga aggaagaacg aaggaaggag cacagactta
     7741 gattggtata tatacgcata tgtagtgttg aagaaacatg aaattgccca gtattcttaa
     7801 cccaactgca cagaacaaaa acgtgcagga aacgaagata aatcatgtcg aaagctacat
     7861 ataaggaacg tgctgctact catcctagtc ctgttgctgc caagctattt aatatcatgc
     7921 acgaaaagca aacaaacttg tgtgcttcat tggatgttcg taccaccaag gaattactgg
     7981 agttagttga agcattaggt cccaaaattt gtttactaaa aacacatgtg gatatcttga
     8041 ctgatttttc catggagggc acagttaagc cgctaaaggc attatccgcc aagtacaatt
     8101 ttttactctt cgaggacaga aaatttgctg acattggtaa tacagtcaaa ttgcagtact
     8161 ctgcgggtgt atacagaata gcagaatggg cagacattac gaatgcacac ggtgtggtgg
     8221 gcccaggtat tgttagcggt ttgaagcagg cggcagaaga agtaacaaag gaacctagag
     8281 gccttttgat gttagcagaa ttgtcatgca agggctccct atctactgga gaatatacta
     8341 agggtactgt tgacattgcg aagagcgaca aagattttgt tatcggcttt attgctcaaa
     8401 gagacatggg tggaagagat gaaggttacg attggttgat tatgacaccc ggtgtgggtt
     8461 tagatgacaa gggagatgca ttgggtcaac agtatagaac cgtggatgat gtggtttcta
     8521 caggatctga cattattatt gttggaagag gactatttgc aaagggaagg gatgctaagg
     8581 tagagggtga acgttacaga aaagcaggct gggaagcata tttgagaaga tgcggccagc
     8641 aaaactaaaa aactgtatta taagtaaatg catgtatact aaactcacaa attagagctt
     8701 caatttaatt atatcagtta ttacccgagt aacgaagcat ctgtgcttca ttttgtagaa
     8761 caaaaatgca acgcgagagc gctaattttt caaacaaaga atctgagctg catttttaca
     8821 gaacagaaat gcaacgcgaa agcgctattt taccaacgaa gaatctgtgc ttcatttttg
     8881 taaaacaaaa atgcaacgcg agagcgctaa tttttcaaac aaagaatctg agctgcattt
     8941 ttacagaaca gaaatgcaac gcgagagcgc tattttacca acaaagaatc tatacttctt
     9001 ttttgttcta caaaaatgca tcccgagagc gctatttttc taacaaagca tcttagatta
     9061 ctttttttct cctttgtgcg ctctataatg cagtctcttg ataacttttt gcactgtagg
     9121 tccgttaagg ttagaagaag gctactttgg tgtctatttt ctcttccata aaaaaagcct
     9181 gactccactt cccgcgttta ctgattacta gcgaagctgc gggtgcattt tttcaagata
     9241 aaggcatccc cgattatatt ctataccgat gtggattgcg catactttgt gaacagaaag
     9301 tgatagcgtt gatgattctt cattggtcag aaaattatga acggtttctt ctattttgtc
     9361 tctatatact acgtatagga aatgtttaca ttttcgtatt gttttcgatt cactctatga
     9421 atagttctta ctacaatttt tttgtctaaa gagtaatact agagataaac ataaaaaatg
     9481 tagaggtcga gtttagatgc aagttcaagg agcgaaaggt ggatgggtag gttatatagg
     9541 gatatagcac agagatatat agcaaagaga tacttttgag caatgtttgt ggaagcggta
     9601 ttcgcaatat tttagtagct cgttacagtc cggtgcgttt ttggtttttt gaaagtgcgt
     9661 catcagagcg cttttggttt tcaaaagcgc tctgaagttc ctatactttc tagctagaga
     9721 ataggaactt cccgagcggc cgcgtgttac aagcttgact ccagcgtaac tggactgcaa
     9781 tcaactcact ggctcacctt cacgggtggg cctttcttcg gtagaaaatc aaaggatctt
     9841 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     9901 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgagg taactggctt
     9961 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
    10021 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
    10081 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
    10141 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
    10201 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
    10261 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
    10321 gcttccaggg gtaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
    10381 tgagcatcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
    10441 cgcagaaagg cccacccgaa ggtgagccag gtgattacat ttgggccctc atcagaggtt
    10501 ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg
    10561 aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag
    10621 cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt
    10681 cacttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
    10741 catagttgcc tgactccccg tcgtgtagat aactacgatg cgggagggct taccatctgg
    10801 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
    10861 aaaccagcca gccgggaggg ccgagcgcag aagtgatcct gcaactttat ccgcctccat
    10921 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
    10981 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
    11041 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
    11101 agcggttagc tccttcggtc ctccgatcgt tgccagaagt aagttggccg cagtgttatc
    11161 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg tgagatgctt
    11221 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
    11281 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
    11341 gctcatcatt ggaaaacgtt cttcggggcg taaactctca aggatcttac cgctgttgag
    11401 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
    11461 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    11521 gacacggaaa tgttgaatac tcattttagc ttccttagct cctgaaaatc tcgataactc
    11581 aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg
    11641 ccgatcaagt caaaagcctc cggtcggagg cttttgactt tctgctatgg aggtcaggta
    11701 tgatttaaat ggtcagttag a
//
