LOCUS       sequence-29811-e         746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Beskow A, Grimberg KB, Bott LC, Salomons FA, Dantuma NP, Young P
  TITLE     A conserved unfoldase activity for the p97 AAA-ATPase in proteasomal
            degradation.
  JOURNAL   J Mol Biol. 2009 Dec 11. 394(4):732-46.
  PUBMED    19782090
REFERENCE   2  (bases 1 to 746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2009 Dec 11. 394(4):732-46."
COMMENT     Sequence Label: UbG76V-eGFP-V5His_20aa
FEATURES             Location/Qualifiers
     source          1..746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             116..133
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    258..379
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(295..314)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     349..368
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(523..544)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ccggcccccg gcaggaagag ctgtacagtc atgtcggccg ctcgagtcta gagggccctt
       61 cgaaggtaag cctatcccta accctctctg aggtctcgat tctacgcgta ccggtcatca
      121 tcaccatcac cattgagcgg ccgcgactct agatcataat cagccatacc acatttgtag
      181 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
      241 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
      301 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
      361 aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg
      421 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
      481 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
      541 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
      601 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
      661 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
      721 gtggcgagaa ggaaggaaga agcgaa
//
