LOCUS       sequence-29812-e         692 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Beskow A, Grimberg KB, Bott LC, Salomons FA, Dantuma NP, Young P
  TITLE     A conserved unfoldase activity for the p97 AAA-ATPase in proteasomal
            degradation.
  JOURNAL   J Mol Biol. 2009 Dec 11. 394(4):732-46.
  PUBMED    19782090
REFERENCE   2  (bases 1 to 692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2009 Dec 11. 394(4):732-46."
COMMENT     Sequence Label: UbG76V-eGFP-V5His_20aa
FEATURES             Location/Qualifiers
     source          1..692
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             44..268
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRG"
     regulatory      305..314
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(356..377)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 agatccgcta gcgctaccgg actcagatct cgagctcgga tccatgcaga tcttcgtgaa
       61 gactctgact ggtaagacca tcaccctcga ggttgagccc agtgacacca ttgagaatgt
      121 caaggcaaag atccaagata aggaaggcat ccctcctgac cagcagaggc tgatctttgc
      181 tggaaaacag ctggaagatg ggcgcaccct gtctgactac aacatccaga aagagtccac
      241 cctgcacctg gtactccgtc tcagaggtgt ggtggggaag cttggtcgac aggatccacc
      301 ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
      361 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
      421 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
      481 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
      541 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
      601 caccatcttc ttcaaggacg acggcnacta nngacccgcg ccgaggtgaa gttcgagggc
      661 gacaccctgg tgaaccgcat cgagctgaag gc
//
