LOCUS       sequence-29813-e         701 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Beskow A, Grimberg KB, Bott LC, Salomons FA, Dantuma NP, Young P
  TITLE     A conserved unfoldase activity for the p97 AAA-ATPase in proteasomal
            degradation.
  JOURNAL   J Mol Biol. 2009 Dec 11. 394(4):732-46.
  PUBMED    19782090
REFERENCE   2  (bases 1 to 701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2009 Dec 11. 394(4):732-46."
COMMENT     Sequence Label: UbG76V-eGFP-V5His_20aa
FEATURES             Location/Qualifiers
     source          1..701
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(64..288)
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRG"
     promoter        complement(348..551)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(377..397)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gctctcgccc ttgctcacca tggtggcgac cggtggatcc tgtcgaccaa gcttccccac
       61 cacacctctg agacggagta ccaggtgcag ggtggactct ttctggatgt tgtagtcaga
      121 cagggtgcgc ccatcttcca gctgttttcc agcaaagatc agcctctgct ggtcaggagg
      181 gatgccttcc ttatcttgga tctttgcctt gacattctca atggtgtcac tgggctcaac
      241 ctcgagggtg atggtcttac cagtcagagt cttcacgaag atctgcatgg atccgagctc
      301 gagatctgag tccggtagcg ctagcggatc tgacggttca ctaaaccagc tctgcttata
      361 tagacctccc accgtacacg cctaccgccc atttgcgtca atggggcgga gttgttacga
      421 cattttggaa agtcccgttg attttggtgc caaaacaaac tcccattgac gtcaatgggg
      481 tggagacttg gaaatccccg tgagtcaaac cgctatccac gcccattgat gtactgccaa
      541 aaccgcatca ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag
      601 tcccataagg tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca
      661 atagggggcg tacttggcat atgatacact tgatgtactg c
//
