LOCUS       sequence-298157-        4376 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EMMA Kit # 1000000119 extension.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4376)
  TITLE     Lewin Lab Plasmids
REFERENCE   2  (bases 1 to 4376)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRDAX-VALENTIA-017   NLSNoLS-SuperNova//BsaI
FEATURES             Location/Qualifiers
     source          1..4376
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             168..215
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     polyA_signal    1086..1207
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1123..1142)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1177..1196
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     terminator      2008..2037
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      2059..2646
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2547..2566
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2658..2685)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(join(2709..3500,3501..3569))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3332..3351
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3570..3672)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(3673..3704)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     enhancer        3860..4239
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ggggcggggc gaggggcggg gcggggcgag gcggagaggt gcggcggcag ccaatcagag
       61 cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa
      121 gcgaagcgcg cggcgggcgg gagtcgctgc gttgggacgc caccatgaaa cggcctgctg
      181 ccaccaagaa agcaggacag gccaagaaga agaaacgcag gagaaggcga cgtagaggcg
      241 gtagcggggg cagcgaggta ggtccagctc tgtttcagtc cgatatgacg tttaagatct
      301 tcatcgatgg cgaagtgaac ggtcagaagt tcaccatagt tgctgatggc tcttccaagt
      361 tcccacatgg cgacttcaat gcccatgcag tgtgtgaaac tgggaaactg cctatgagct
      421 ggaaacccat ttgccatctg attcaatggg gagaaccgtt cttcgcgagg tatcctgatg
      481 gcattagcca ctttgcccag gagtgctttc cagaggggct gagtatcgac cgtactgtgc
      541 gcttcgagaa tgacgggaca atgacctctc accacaccta cgagttggac gacacatgtg
      601 tggtcagcag gatcacggtg aactgtgacg gctttcagcc agacggacct atcatgcgcg
      661 atcagctcgt tgacatcctt ccctcagaaa cacacatgtt tccgcatgga cctaatgccg
      721 tcagacagac agcaactatt gggtttacca ctgcagatgg cgggaagatg atgggacact
      781 tcgacagcaa gatgaccttc aacggcagta gagccataga gattcctgga ccccatttcg
      841 tgaccatcat caccaaacag acacgggaca catccgacaa acgagatcac gtctgccaaa
      901 gggaagtcgc ttacgctcac tctgtacccc ggattacttc agccataggt agcgatgagg
      961 attcaggctc tgattaaggc gccaggtaca tctccaatac ggcgtcttgc atagagaaca
     1021 cgattagcac accggttacc cacgtgatgc ccgaacggac ccatgtcatg ccagagcgca
     1081 ctggtaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     1141 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     1201 tatcttatca tgtctgtcgt cgaattcgag ttggcgcgcc tgtcattcta aatctctctt
     1261 tcagcctaaa gctttttccc cgtatccccc caggtgtctg caggctcaaa gagcagcgag
     1321 aagcgttcag aggaaagcga tcccgtgcca ccttccccgt gcccgggctg tccccgcacg
     1381 ctgccggctc ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg gcggctcgct
     1441 gctgccccct agcgggggag ggacgtaatt acatccctgg gggctttggg agggggctgt
     1501 ccccgtgagc tcccaggcgc gcctgtcatt ctaaatctct ctttcagcct aaagcttttt
     1561 ccccgtatcc ccccaggtgt ctgcaggctc aaagagcagc gagaagcgtt cagaggaaag
     1621 cgatcccgtg ccaccttccc cgtgcccggg ctgtccccgc acgctgccgg ctcggggatg
     1681 cggggggagc gccggaccgg agcggagccc cgggcggctc gctgctgccc cctagcgggg
     1741 gagggacgta attacatccc tgggggcttt gggggggggc tgtccccgtg agctcccagg
     1801 cgcgccaact cggtacctca cccgattctc atcatggaga cacatcagga catctccaat
     1861 accgccatgg aaacgcacca agatgcgact gctcttctgc cagtgacggc attcgtctgc
     1921 aagattaacg gcatacagaa agaaacgatg agacccattc aggacgagcc tcagactcca
     1981 gcgtaactgg actgcaatca actcactggc tcaccttcac gggtgggcct ttcttcggta
     2041 gaaaatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     2101 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     2161 tccgaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     2221 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     2281 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     2341 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     2401 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     2461 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     2521 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     2581 tttcgccacc tctgacttga gcatcgattt ttgtgatgct cgtcaggggg gcggagccta
     2641 tggaaaaacg ccagcaacgc agaaaggccc acccgaaggt gagccaggtg attacatttg
     2701 ggccctcatt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     2761 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatgcggga gggcttacca
     2821 tctggcccca gtgctgcaat gataccgcga gaaccacgct caccggctcc agatttatca
     2881 gcaataaacc agccagccgg gagggccgag cgcagaagtg atcctgcaac tttatccgcc
     2941 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     3001 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     3061 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     3121 aaaaaagcgg ttagctcctt cggtcctccg atcgttgcca gaagtaagtt ggccgcagtg
     3181 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtgaga
     3241 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     3301 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     3361 aaagtgctca tcattggaaa acgttcttcg gggcgtaaac tctcaaggat cttaccgctg
     3421 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     3481 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     3541 agggcgacac ggaaatgttg aatactcatt ttagcttcct tagctcctga aaatctcgat
     3601 aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt
     3661 acgtgccgat caagtcaaaa gcctccggtc ggaggctttt gactttctgc tatggaggtc
     3721 aggtatgatt taaaggtctc ataggtcttg tattcagtct cgcactttag acatggtctt
     3781 ctgctgcccg tggtcctcta ttcgtcggat gttctgtcga ttcttcctgt ctatctggac
     3841 tggatctgat atcatcgtcg acattgatta ttgactagtt attaatagta atcaattacg
     3901 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     3961 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     4021 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     4081 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     4141 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     4201 tggcagtaca tctacgtatt agtcatcgct attaccatgg tcgaggtgag ccccacgttc
     4261 tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt atttattttt
     4321 taattatttt gtgcagcgat gggggcgggg ggggggggnn nnncgccagg cggggc
//
