LOCUS       sequence-298158-        5724 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EMMA Kit # 1000000119 extension.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5724)
  TITLE     Lewin Lab Plasmids
REFERENCE   2  (bases 1 to 5724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRDAX-VALENTIA-015   Vimentin-Neptune//BsaI
FEATURES             Location/Qualifiers
     source          1..5724
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        158..537
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        539..814
                     /label=chicken beta-actin promoter
     polyA_signal    3108..3229
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3145..3164)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3199..3218
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     terminator      4030..4059
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      4081..4668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4569..4588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(4680..4707)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(join(4731..5522,5523..5591))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5354..5373
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5592..5694)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(join(5695..5724,1..2))
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 ctttctgcta tggaggtcag gtatgattta aaggtctcat aggtcttgta ttcagtctcg
       61 cactttagac atggtcttct gctgcccgtg gtcctctatt cgtcggatgt tctgtcgatt
      121 cttcctgtct atctggactg gatctgatat catcgtcgac attgattatt gactagttat
      181 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
      241 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      301 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      361 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      421 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
      481 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
      541 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
      601 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg ggggggggcg
      661 cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga gaggtgcggc
      721 ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg
      781 gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgttgg gacgccacca
      841 tgagcaccag gagtgtcagt agctcctcct ataggcgtat gtttggcgga cctggaacag
      901 cttctcgacc cagttcatct cggtcatacg tcacgacttc aacacgaacc tatagcctgg
      961 gaagtgcgct taggcctagc actagtcgca gcttgtacgc atcttctcca ggtggggtgt
     1021 atgccacgcg ttctagtgcc gtgagactgc gttcttctgt tcctggcgta agactcctgc
     1081 aggattcagt cgacttcagc cttgcagacg ccatcaacac cgagttcaag aataccagga
     1141 ctaacgagaa ggtggagctg caggagctga atgatcggtt cgctaactac atcgataaag
     1201 tgagatttct cgagcagcag aacaagattc tgctggcaga gctggaacag ctgaaagggc
     1261 aaggcaaatc cagactcgga gacttgtacg aggaggaaat gcgggagctc aggcgacaag
     1321 tggatcagct gacaaacgac aaagctcgcg tggaagtcga gagagacaat ctggccgaag
     1381 atatcatgcg gcttcgggag aagttgcagg aagaaatgct gcagcgagag gaggcggaga
     1441 acacactcca gtcctttcgc caggatgtgg acaatgcttc cctggcacgg ctggacctcg
     1501 agaggaaagt tgaaagcctc caggaagaga tagcctttct caagaaactg cacgaagagg
     1561 aaatccagga actgcaagct cagattcagg agcaacacgt gcagattgat gtggacgtgt
     1621 ctaagcccga ccttaccgct gcactccgcg atgtccggca gcagtacgag tcagttgccg
     1681 ctaagaactt gcaagaagca gaggaatggt acaagagcaa gttcgctgac ctgagcgaag
     1741 cagcgaatcg caacaatgac gctcttagac aagccaagca ggagtctacg gagtatcgca
     1801 gacaggttca atccctgact tgcgaagttg atgccctgaa ggggaccaat gagagcttgg
     1861 aacgacaaat gcgtgagatg gaggaaaatt tcgccgtaga agctgccaac tatcaggaca
     1921 ctataggtcg cctgcaagac gagattcaga acatgaagga ggagatggcc aggcatctca
     1981 gggagtacca ggatctgctc aatgtcaaaa tggccctgga tatcgagatc gcaacttatc
     2041 ggaaactgct ggaaggcgag gagagtagaa tttcccttcc cttgccgaac ttctccagcc
     2101 tgaatcttag ggaaaccaac ctggactcac tgccactggt agacacacac tccaaaagga
     2161 ccctgttgat caagacagtg gaaaccagag atggccaggt gatcaacgaa acaagccagc
     2221 atcacgatga cctggagggc gggtcaggcg gcagcgtcag caaaggcgag gagctcatca
     2281 aggagaacat gcacaccaag ctgtacatgg aaggtactgt gaataaccac cacttcaaat
     2341 gcacgcatga aggtgaaggc aagccctatg agggcacaca gaccaatcgc atcaaagtgg
     2401 ttgagggagg tcctctgccg tttgcgttcg acattctggc cacatgcttc atgtatggct
     2461 ccaaaacgtt catcaaccac actcagggca tacccgattt cttcaagcag tcttttcctg
     2521 aaggctttac atgggagaga gtgaccacat acgaagatgg aggcgtcctc actgtgacac
     2581 aagacacaag tctgcaggat ggatgtctca tctacaatgt gaagctgcgt ggggtgaact
     2641 ttccctctaa tgggcctgta atgcagaaga aaactcttgg gtgggaagca tcaaccgaga
     2701 ctctttaccc agctgatgga gggcttgaag gccggtgtga catggctctc aagttggtag
     2761 gtggaggaca tctgcattgc aacctgaaaa ccacctacag gagcaagaag ccagcaaaga
     2821 acctgaagat gccaggagtc tattttgtgg acagacgatt ggagcggatt aaggaggccg
     2881 acaatgaaac ctatgtcgag caacacgagg ttgccgttgc taggtattgt gacttgcctt
     2941 ccaaactggg gcataagctg aacgggatgg atgagctgta caaatcaggc tctgattaag
     3001 gcgccaggta catctccaat acggcgtctt gcatagagaa cacgattagc acaccggtta
     3061 cccacgtgat gcccgaacgg acccatgtca tgccagagcg cactggtaac ttgtttattg
     3121 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     3181 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgtc
     3241 gtcgaattcg agttggcgcg cctgtcattc taaatctctc tttcagccta aagctttttc
     3301 cccgtatccc cccaggtgtc tgcaggctca aagagcagcg agaagcgttc agaggaaagc
     3361 gatcccgtgc caccttcccc gtgcccgggc tgtccccgca cgctgccggc tcggggatgc
     3421 ggggggagcg ccggaccgga gcggagcccc gggcggctcg ctgctgcccc ctagcggggg
     3481 agggacgtaa ttacatccct gggggctttg ggagggggct gtccccgtga gctcccaggc
     3541 gcgcctgtca ttctaaatct ctctttcagc ctaaagcttt ttccccgtat ccccccaggt
     3601 gtctgcaggc tcaaagagca gcgagaagcg ttcagaggaa agcgatcccg tgccaccttc
     3661 cccgtgcccg ggctgtcccc gcacgctgcc ggctcgggga tgcgggggga gcgccggacc
     3721 ggagcggagc cccgggcggc tcgctgctgc cccctagcgg gggagggacg taattacatc
     3781 cctgggggct ttgggggggg gctgtccccg tgagctccca ggcgcgccaa ctcggtacct
     3841 cacccgattc tcatcatgga gacacatcag gacatctcca ataccgccat ggaaacgcac
     3901 caagatgcga ctgctcttct gccagtgacg gcattcgtct gcaagattaa cggcatacag
     3961 aaagaaacga tgagacccat tcaggacgag cctcagactc cagcgtaact ggactgcaat
     4021 caactcactg gctcaccttc acgggtgggc ctttcttcgg tagaaaatca aaggatcttc
     4081 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     4141 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaggt aactggcttc
     4201 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     4261 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     4321 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     4381 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     4441 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     4501 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     4561 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     4621 gagcatcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     4681 gcagaaaggc ccacccgaag gtgagccagg tgattacatt tgggccctca ttaccaatgc
     4741 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     4801 ctccccgtcg tgtagataac tacgatgcgg gagggcttac catctggccc cagtgctgca
     4861 atgataccgc gagaaccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     4921 gggagggccg agcgcagaag tgatcctgca actttatccg cctccatcca gtctattaat
     4981 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     5041 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     5101 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     5161 ttcggtcctc cgatcgttgc cagaagtaag ttggccgcag tgttatcact catggttatg
     5221 gcagcactgc ataattctct tactgtcatg ccatccgtga gatgcttttc tgtgactggt
     5281 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     5341 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     5401 aaacgttctt cggggcgtaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     5461 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     5521 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     5581 tgaatactca ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc
     5641 ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccg atcaagtcaa
     5701 aagcctccgg tcggaggctt ttga
//
