LOCUS       sequence-298211-        5363 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid carries fucO and aldA under the control of P_gyrA 
            promoter, which is used for study about Improving ethylene glycol 
            utilization in Escherichia coli fermentation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5363)
  AUTHORS   Panda S, Fung V, Zhou J, Liang H, Zhou K
  TITLE     Improving ethylene glycol utilization in Escherichia coli 
            fermentation
  JOURNAL   Biochemical Engineering Journal
REFERENCE   2  (bases 1 to 5363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochemical
            Engineering Journal"
COMMENT     Sequence Label: pEG03
FEATURES             Location/Qualifiers
     source          1..5363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             211..233
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             complement(3271..3930)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3845..3864
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(3931..4033)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(4442..4459)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4576..5121)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(4656..4675)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaggcgacgg cttcaaattt agcgatctct tcgtggtcta cgttatggtt ttccggcgat
       61 ttttcggcat tcattggcac ttctactccg taattggcaa gacaaacgag tatatcaggc
      121 attggatgtg aataaagcgt ataggtttac ctcaaactgc gcggctgtgt tataatttgc
      181 gacctttgaa tccgggatac tagaaataat tttgtttaac tttaagaagg agatatacat
      241 atggctaaca gaatgattct gaacgaaacg gcatggtttg gtcggggtgc tgttggggct
      301 ttaaccgatg aggtgaaacg ccgtggttat cagaaggcgc tgatcgtcac cgataaaacg
      361 ctggtgcaat gcggcgtggt ggcgaaagtg accgataaga tggatgctgc agggctggca
      421 tgggcgattt acgacggcgt agtgcccaac ccaacaatta ctgtcgtcaa agaagggctc
      481 ggtgtattcc agaatagcgg cgcggattac ctgatcgcta ttggtggtgg ttctccacag
      541 gatacttgta aagcgattgg cattatcagc aacaacccgg agtttgccga tgtgcgtagc
      601 ctggaagggc tttccccgac caataaaccc agtgtaccga ttctggcaat tcctaccaca
      661 gcaggtactg cggcagaagt gaccattaac tacgtgatca ctgacgaaga gaaacggcgc
      721 aagtttgttt gcgttgatcc gcatgatatc ccgcaggtgg cgtttattga cgctgacatg
      781 atggatggta tgcctccagc gctgaaagct gcgacgggtg tcgatgcgct cactcatgct
      841 attgaggggt atattacccg tggcgcgtgg gcgctaaccg atgcactgca cattaaagcg
      901 attgaaatca ttgctggggc gctgcgagga tcggttgctg gtgataagga tgccggagaa
      961 gaaatggcgc tcgggcagta tgttgcgggt atgggcttct cgaatgttgg gttagggttg
     1021 gtgcatggta tggcgcatcc actgggcgcg ttttataaca ctccacacgg tgttgcgaac
     1081 gccatcctgt taccgcatgt catgcgttat aacgctgact ttaccggtga gaagtaccgc
     1141 gatatcgcgc gcgttatggg cgtgaaagtg gaaggtatga gcctggaaga ggcgcgtaat
     1201 gccgctgttg aagcggtgtt tgctctcaac cgtgatgtcg gtattccgcc acatttgcgt
     1261 gatgttggtg tacgcaagga agacattccg gcactggcgc aggcggcact ggatgatgtt
     1321 tgtaccggtg gcaacccgcg tgaagcaacg cttgaggata ttgtagagct ttaccatacc
     1381 gcctggtaac cgttcattta tcacaaaagg attgttcgat gtcagtaccc gttcaacatc
     1441 ctatgtatat cgatggacag tttgttacct ggcgtggaga cgcatggatt gatgtggtaa
     1501 accctgctac agaggctgtc atttcccgca tacccgatgg tcaggccgag gatgcccgta
     1561 aggcaatcga tgcagcagaa cgtgcacaac cagaatggga agcgttgcct gctattgaac
     1621 gcgccagttg gttgcgcaaa atctccgccg ggatccgcga acgcgccagt gaaatcagtg
     1681 cgctgattgt tgaagaaggg ggcaagatcc agcagctggc tgaagtcgaa gtggctttta
     1741 ctgccgacta tatcgattac atggcggagt gggcacggcg ttacgagggc gagattattc
     1801 aaagcgatcg tccaggagaa aatattcttt tgtttaaacg tgcgcttggt gtgactaccg
     1861 gcattctgcc gtggaacttc ccgttcttcc tcattgcccg caaaatggct cccgctcttt
     1921 tgaccggtaa taccatcgtc attaaaccta gtgaatttac gccaaacaat gcgattgcat
     1981 tcgccaaaat cgtcgatgaa ataggccttc cgcgcggcgt gtttaacctt gtactggggc
     2041 gtggtgaaac cgttgggcaa gaactggcgg gtaacccaaa ggtcgcaatg gtcagtatga
     2101 caggcagcgt ctctgcaggt gagaagatca tggcgactgc ggcgaaaaac atcaccaaag
     2161 tgtgtctgga attggggggt aaagcaccag ctatcgtaat ggacgatgcc gatcttgaac
     2221 tggcagtcaa agccatcgtt gattcacgcg tcattaatag tgggcaagtg tgtaactgtg
     2281 cagaacgtgt ttatgtacag aaaggcattt atgatcagtt cgtcaatcgg ctgggtgaag
     2341 cgatgcaggc ggttcaattt ggtaaccccg ctgaacgcaa cgacattgcg atggggccgt
     2401 tgattaacgc cgcggcgctg gaaagggtcg agcaaaaagt ggcgcgcgca gtagaagaag
     2461 gggcgagagt ggcgttcggt ggcaaagcgg tagaggggaa aggatattat tatccgccga
     2521 cattgctgct ggatgttcgc caggaaatgt cgattatgca tgaggaaacc tttggcccgg
     2581 tgctgccagt tgtcgcattt gacacgctgg aagatgctat ctcaatggct aatgacagtg
     2641 attacggcct gacctcatca atctataccc aaaatctgaa cgtcgcgatg aaagccatta
     2701 aagggctgaa gtttggtgaa acttacatca accgtgaaaa cttcgaagct atgcaaggct
     2761 tccacgccgg atggcgtaaa tccggtattg gcggcgcaga tggtaaacat ggcttgcatg
     2821 aatatctgca gacccaggtg gtttatttac agtcttgatg ggctgaaggg tttaaggcga
     2881 agacgaacaa taaggcctcc caaatcgggg ggcctttttt attgataaca aaaaggcaac
     2941 actatgacat cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc gctggatgaa
     3001 aaattattag cattactggc agagcggcgc gaattcaaac agaaaggcca tggctggcct
     3061 caggcatttg agaagcacac ggtcacactg cttccggtag tcaataaacc ggtaaaccag
     3121 caatagacat aagcggctat ttaacgaccc tgccctgaac cgacgaccgg gtcgaatttg
     3181 ctttcgaatt tctgccattc atccgcttat tatcacttat tcaggcgtag caccaggcgt
     3241 ttaagggcac caataactgc cttaaaaaaa ttacgccccg ccctgccact catcgcagta
     3301 ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacagacgg catgatgaac
     3361 ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa
     3421 aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac
     3481 ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag
     3541 gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc
     3601 gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca
     3661 agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac ggaattccgg
     3721 atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt
     3781 tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca
     3841 ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac
     3901 ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga
     3961 taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct
     4021 tacgtgccga tcaacgtctc attttcgcca aaagttggcc cagggcttcc cggtatcaac
     4081 agggacacca ggatttattt attctgcgaa gtgatcttcc gtcacaggta tttattcggc
     4141 gcaaagtgcg tcgggtgatg ctgccaactt actgatttag tgtatgatgg tgtttttgag
     4201 gtgctccagt ggcttctgtt tctatcagct gtcccttcac acaacatagc cacggtgttc
     4261 agctactgac ggggtggtgc gtaacggcaa aagcaccgcc ggacatcagc gctagcggag
     4321 tgtatactgg cttactatgt tggcactgat gagggtgtca gtgaagtgct tcatgtggca
     4381 ggagaaaaaa ggctgcaccg gtgcgtcagc agaatatgtg atacaggata tattccgctt
     4441 cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg
     4501 aacggggcgg agatttcctg gaagatgcca ggaagatact taacagggaa gtgagagggc
     4561 cgcggcaaag ccgtttttcc ataggctccg cccccctgac aagcatcacg aaatctgacg
     4621 ctcaaatcag tggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     4681 cggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca ttccgctgtt
     4741 atggccgcgt ttgtctcatt ccacgcctga cactcagttc cgggtaggca gttcgctcca
     4801 agctggactg tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta tccggtaact
     4861 atcgtcttga gtccaacccg gaaagacatg caaaagcacc actggcagca gccactggta
     4921 attgatttag aggagttagt cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca
     4981 agttttggtg actgcgctcc tccaagccag ttacctcggt tcaaagagtt ggtagctcag
     5041 agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt tttcagagca agagattacg
     5101 cgcagaccaa aacgatctca agaagatcat cttattaatc agataaaata tttctagatt
     5161 tcagtgcaat ttatctcttc aaatgtagca cctgaagtca gccccatacg atataagttg
     5221 taattctcat gtttgacagc ttatcaccca gtcctgctcg cttcgctact tggccatacc
     5281 cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat
     5341 gtctccctcg actcacactt ggg
//
