LOCUS       sequence-298230-        3076 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast Expression plasmid for SARS-CoV-2 N (nucleocapsid).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3076)
  TITLE     SARS-CoV-2 protein Yeast expression constructs, tagged, for protein 
            expression and/or purification
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4046548
FEATURES             Location/Qualifiers
     source          1..3076
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        25..466
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    25..142
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     437..460
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             complement(667..678)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             1735..1752
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             1753..1776
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     complement(1821..1839)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             2190..2993
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
ORIGIN
        1 gcggccgctt gctatattga agtacggatt agaagccgcc gagcgggcga cagccctccg
       61 acggaagact ctcctccgtg cgtcctcgtc ttcaccggtc gcgttcctga aacgcagatg
      121 tgcctcgcgc cgcactgctc cgaacaataa agattctaca atactagctt ttatggttat
      181 gaagaggaaa aattggcagt aacctggccc cacaaacctt caaattaacg aatcaaatta
      241 acaaccatag gatgataatg cgattagttt tttagcctta tttctggggt aattaatcag
      301 cgaagcgatg atttttgatc tattaacaga tatataaatg gaaaagctgc ataaccactt
      361 taactaatac tttcaacatt ttcagtttgt attacttctt attcaaatgt cataaaagta
      421 tcaacaaaaa attgttaata tacctctata ctttaacgtc aaggagaaaa aactataatg
      481 tctgataatg gcccacagaa ccaacgtaac gcacctagaa tcaccttcgg tggtccatcc
      541 gacagcactg gttcaaatca aaacggagag agatcgggtg cccggagtaa gcaaaggcgc
      601 ccccaaggtc taccgaataa cacagcttct tggtttactg ctcttacgca acacggtaaa
      661 gaagacttga agttcccaag aggtcaaggg gttccaatta acaccaactc ctctccagat
      721 gatcaaatag gttactatag aagagctact agacgtatta ggggtgggga cggcaaaatg
      781 aaggatttat ctcctagatg gtacttttat tacttgggta ccggtccaga agctggattg
      841 ccttacggtg ccaataagga cggtatcatt tgggtagcaa ctgaaggtgc tttaaatacc
      901 ccaaaggacc atatcggtac tagaaaccct gctaataacg ctgccattgt cttgcaatta
      961 ccacagggga caacgttgcc aaagggtttc tacgccgaag gatccagagg tggctcgcaa
     1021 gcttctagta gatcatcctc tcgcagcaga aactcatctc gtaactccac accaggtagt
     1081 tcgagaggta cctctccagc gagaatggct ggtaacggtg gtgatgcagc tctggcgttg
     1141 ctgctactcg acagattgaa tcaattggaa tccaaaatgt ctggtaaggg tcaacaacaa
     1201 cagggtcaga ctgtgactaa gaagtctgct gctgaagctt ccaaaaagcc ccgtcaaaag
     1261 agaaccgcca ccaaggccta taatgtcact caagctttcg gaagaagagg tccagagcaa
     1321 acgcaaggta actttggaga tcaagaattg atccggcagg gtaccgatta taaacactgg
     1381 ccccaaattg ctcaattcgc gccaagcgcc tcagcttttt tcggcatgtc aagaataggt
     1441 atggaagtta ctccatctgg gacttggtta acttacactg gtgcaatcaa gttggacgat
     1501 aaagacccaa attttaaaga ccaagttatt ctactcaaca agcatataga tgcttacaaa
     1561 accttcccgc ctacagaacc caagaaagat aaaaagaaga aggctgacga gacacaagca
     1621 ttgcctcagc gtcaaaagaa acaacaaact gtcaccttgt taccagcagc tgatcttgat
     1681 gatttctcta agcaattgca acaatccatg tcgtccgccg actctactca agcgctcgtg
     1741 ccgagaggtt cttggagcca tccgcagttt gaaaaataaa caggcccctt ttcctttgtc
     1801 gatatcatgt aattagttat gtcacgctta cattcacgcc ctcctcccac atccgctcta
     1861 accgaaaagg aaggagttag acaacctgaa gtctaggtcc ctatttattt tttttaatag
     1921 ttatgttagt attaagaacg ttatttatat ttcaaatttt tctttttttt ctgtacaaag
     1981 cggccgccga accactagtg cactgcagta cacggtttcc ttgaaatttt tttgattcgg
     2041 taatctccga acagaaggaa gaacgaagga aggagcacag acttagattg gtatatatac
     2101 gcatatgtag tgttgaagaa acatgaaatt gcccagtatt cttaacccaa ctgcacagaa
     2161 caaaaacgtg caggaaacga agataaatca tgtcgaaagc tacatataag gaacgtgctg
     2221 ctactcatcc tagtcctgtt gctgccaagc tatttaatat catgcacgaa aagcaaacaa
     2281 acttgtgtgc ttcattggat gttcgtacca ccaaggaatt actggagtta gttgaagcat
     2341 taggtcccaa aatttgttta ctaaaaacac atgtggatat cttgactgat ttttccatgg
     2401 agggcacagt taagccgcta aaggcattat ccgccaagta caatttttta ctcttcgagg
     2461 acagaaaatt tgctgacatt ggtaatacag tcaaattgca gtactctgcg ggtgtataca
     2521 gaatagcaga atgggcagac attacgaatg cacacggtgt ggtgggccca ggtattgtta
     2581 gcggtttgaa gcaggcggca gaagaagtaa caaaggaacc tagaggcctt ttgatgttag
     2641 cagaattgtc atgcaagggc tccctatcta ctggagaata tactaagggt actgttgaca
     2701 ttgcgaagag cgacaaagat tttgttatcg gctttattgc tcaaagagac atgggtggaa
     2761 gagatgaagg ttacgattgg ttgattatga cacccggtgt gggtttagat gacaagggag
     2821 atgcattggg tcaacagtat agaaccgtgg atgatgtggt ttctacagga tctgacatta
     2881 ttattgttgg aagaggacta tttgcaaagg gaagggatgc taaggtagag ggtgaacgtt
     2941 acagaaaagc aggctgggaa gcatatttga gaagatgcgg ccagcaaaac taaaaaactg
     3001 tattataagt aaatgcatgt atactaaact cacaaattag agcttcaatt taattatatc
     3061 agttattacc cgagta
//
