LOCUS       sequence-298250-        2580 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To detect the calcium ion releases close to the lysosomal surface.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Li M, Zhang CS, Zong Y, Feng JW, Ma T, Hu M, Lin Z, Li X, Xie C, Wu 
            Y, Jiang D, Li Y, Zhang C, Tian X, Wang W, Yang Y, Chen J, Cui J, Wu
            YQ, Chen X, Liu QF, Wu J, Lin SY, Ye Z, Liu Y, Piao HL, Yu L, Zhou 
            Z, Xie XS, Hardie DG, Lin SC
  TITLE     Transient Receptor Potential V Channels Are Essential for Glucose 
            Sensing by Aldolase and AMPK.
  JOURNAL   Cell Metab. 2019 Sep 3;30(3):508-524.e12. doi: 
            10.1016/j.cmet.2019.05.018. Epub 2019 Jun 13.
  PUBMED    31204282
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2019.05.018"; journalName: "Cell Metab"; date: 
            "2019-09-3- 3"; volume: "30"; issue: "3"; pages: "508-524.e12"
COMMENT     Sequence Label: pBoBi-hLAMP2-C-GC6s
FEATURES             Location/Qualifiers
     source          1..2580
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1231..1239,1240..1257,1258..1260,1261..1293,1294..1296,
                     1297..1320,1321..1347,1348..1404,1405..1410,1411..1680,
                     1681..1698,1699..2133,2134..2139,2140..2580)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6s
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFHIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFGVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      1327..1336
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1617..1638
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1744..1765)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2005..2024)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atggtgtgct tccgcctctt cccggttccg ggctcagggc tcgttctggt ctgcctagtc
       61 ctgggagctg tgcggtctta tgcattggaa cttaatttga cagattcaga aaatgccact
      121 tgcctttatg caaaatggca gatgaatttc acagttcgct atgaaactac aaataaaact
      181 tataaaactg taaccatttc agaccatggc actgtgacat ataatggaag catttgtggg
      241 gatgatcaga atggtcccaa aatagcagtg cagttcggac ctggcttttc ctggattgcg
      301 aattttacca aggcagcatc tacttattca attgacagcg tctcattttc ctacaacact
      361 ggtgataaca caacatttcc tgatgctgaa gataaaggaa ttcttactgt tgatgaactt
      421 ttggccatca gaattccatt gaatgacctt tttagatgca atagtttatc aactttggaa
      481 aagaatgatg ttgtccaaca ctactgggat gttcttgtac aagcttttgt ccaaaatggc
      541 acagtgagca caaatgagtt cctgtgtgat aaagacaaaa cttcaacagt ggcacccacc
      601 atacacacca ctgtgccatc tcctactaca acacctactc caaaggaaaa accagaagct
      661 ggaacctatt cagttaataa tggcaatgat acttgtctgc tggctaccat ggggctgcag
      721 ctgaacatca ctcaggataa ggttgcttca gttattaaca tcaaccccaa tacaactcac
      781 tccacaggca gctgccgttc tcacactgct ctacttagac tcaatagcag caccattaag
      841 tatctagact ttgtctttgc tgtgaaaaat gaaaaccgat tttatctgaa ggaagtgaac
      901 atcagcatgt atttggttaa tggctccgtt ttcagcattg caaataacaa tctcagctac
      961 tgggatgccc ccctgggaag ttcttatatg tgcaacaaag agcagactgt ttcagtgtct
     1021 ggagcatttc agataaatac ctttgatcta agggttcagc ctttcaatgt gacacaagga
     1081 aagtattcta cagcccaaga gtgttcgctg gatgatgaca ccattctaat cccaattata
     1141 gttggtgctg gtctttcagg cttgattatc gttatagtga ttgcttacgt aattggcaga
     1201 agaaaaagtt atgctggata tcagactctg atgggttctc atcatcatca tcatcatggt
     1261 atggctagca tgactggtgg acagcaaatg ggtcgggatc tgtacgacga tgacgataag
     1321 gatctcgcca ccatggtcga ctcatcacgt cgtaagtgga ataagacagg tcacgcagtc
     1381 agagctatag gtcggctgag ctcactcgag aacgtctata tcaaggccga caagcagaag
     1441 aacggcatca aggcgaactt ccacatccgc cacaacatcg aggacggcgg cgtgcagctc
     1501 gcctaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     1561 cactacctga gcgtgcagtc caaactttcg aaagacccca acgagaagcg cgatcacatg
     1621 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     1681 ggcggtaccg gagggagcat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     1741 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggt
     1801 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     1861 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     1921 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacatc
     1981 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     2041 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     2101 ggcaacatcc tggggcacaa gctggagtac aacctgccgg accaactgac tgaagagcag
     2161 atcgcagaat ttaaagaggc tttctcccta tttgacaagg acggggatgg gacaataaca
     2221 accaaggagc tggggacggt gatgcggtct ctggggcaga accccacaga agcagagctg
     2281 caggacatga tcaatgaagt agatgccgac ggtgacggca caatcgactt ccctgagttc
     2341 ctgacaatga tggcaagaaa aatgaaatac agggacacgg aagaagaaat tagagaagcg
     2401 ttcggtgtgt ttgataagga tggcaatggc tacatcagtg cagcagagct tcgccacgtg
     2461 atgacaaacc ttggagagaa gttaacagat gaagaggttg atgaaatgat cagggaagca
     2521 gacatcgatg gggatggtca ggtaaactac gaagagtttg tacaaatgat gacagcgaag
//
