LOCUS       sequence-298289-        5363 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid carries fucO and aldA under the control of P_gyrA 
            promoter, which is used for study about Improving ethylene glycol 
            utilization in Escherichia coli fermentation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5363)
  AUTHORS   Panda S, Fung V, Zhou J, Liang H, Zhou K
  TITLE     Improving ethylene glycol utilization in Escherichia coli 
            fermentation
  JOURNAL   Biochemical Engineering Journal
REFERENCE   2  (bases 1 to 5363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochemical
            Engineering Journal"
COMMENT     Sequence Label: pEG03
FEATURES             Location/Qualifiers
     source          1..5363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(416..1075)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     990..1009
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1076..1178)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(1587..1604)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1721..2266)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(1801..1820)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     RBS             2719..2741
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 tgatgggctg aagggtttaa ggcgaagacg aacaataagg cctcccaaat cggggggcct
       61 tttttattga taacaaaaag gcaacactat gacatcggaa aacccgttac tggcgctgcg
      121 agagaaaatc agcgcgctgg atgaaaaatt attagcatta ctggcagagc ggcgcgaatt
      181 caaacagaaa ggccatggct ggcctcaggc atttgagaag cacacggtca cactgcttcc
      241 ggtagtcaat aaaccggtaa accagcaata gacataagcg gctatttaac gaccctgccc
      301 tgaaccgacg accgggtcga atttgctttc gaatttctgc cattcatccg cttattatca
      361 cttattcagg cgtagcacca ggcgtttaag ggcaccaata actgccttaa aaaaattacg
      421 ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga
      481 agccatcaca gacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt
      541 gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt
      601 ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac atattctcaa
      661 taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata
      721 tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag
      781 tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt
      841 ctttcattgc catacggaat tccggatgag cattcatcag gcgggcaaga atgtgaataa
      901 aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca
      961 gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt
     1021 tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag
     1081 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt
     1141 cattatggtg aaagttggaa cctcttacgt gccgatcaac gtctcatttt cgccaaaagt
     1201 tggcccaggg cttcccggta tcaacaggga caccaggatt tatttattct gcgaagtgat
     1261 cttccgtcac aggtatttat tcggcgcaaa gtgcgtcggg tgatgctgcc aacttactga
     1321 tttagtgtat gatggtgttt ttgaggtgct ccagtggctt ctgtttctat cagctgtccc
     1381 ttcacacaac atagccacgg tgttcagcta ctgacggggt ggtgcgtaac ggcaaaagca
     1441 ccgccggaca tcagcgctag cggagtgtat actggcttac tatgttggca ctgatgaggg
     1501 tgtcagtgaa gtgcttcatg tggcaggaga aaaaaggctg caccggtgcg tcagcagaat
     1561 atgtgataca ggatatattc cgcttcctcg ctcactgact cgctacgctc ggtcgttcga
     1621 ctgcggcgag cggaaatggc ttacgaacgg ggcggagatt tcctggaaga tgccaggaag
     1681 atacttaaca gggaagtgag agggccgcgg caaagccgtt tttccatagg ctccgccccc
     1741 ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg gcgaaacccg acaggactat
     1801 aaagatacca ggcgtttccc cctggcggct ccctcgtgcg ctctcctgtt cctgcctttc
     1861 ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc tcattccacg cctgacactc
     1921 agttccgggt aggcagttcg ctccaagctg gactgtatgc acgaaccccc cgttcagtcc
     1981 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggaaag acatgcaaaa
     2041 gcaccactgg cagcagccac tggtaattga tttagaggag ttagtcttga agtcatgcgc
     2101 cggttaaggc taaactgaaa ggacaagttt tggtgactgc gctcctccaa gccagttacc
     2161 tcggttcaaa gagttggtag ctcagagaac cttcgaaaaa ccgccctgca aggcggtttt
     2221 ttcgttttca gagcaagaga ttacgcgcag accaaaacga tctcaagaag atcatcttat
     2281 taatcagata aaatatttct agatttcagt gcaatttatc tcttcaaatg tagcacctga
     2341 agtcagcccc atacgatata agttgtaatt ctcatgtttg acagcttatc acccagtcct
     2401 gctcgcttcg ctacttggcc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc
     2461 gagcccgatc ttccccatcg gtgatgtctc cctcgactca cacttgggga ggcgacggct
     2521 tcaaatttag cgatctcttc gtggtctacg ttatggtttt ccggcgattt ttcggcattc
     2581 attggcactt ctactccgta attggcaaga caaacgagta tatcaggcat tggatgtgaa
     2641 taaagcgtat aggtttacct caaactgcgc ggctgtgtta taatttgcga cctttgaatc
     2701 cgggatacta gaaataattt tgtttaactt taagaaggag atatacatat ggctaacaga
     2761 atgattctga acgaaacggc atggtttggt cggggtgctg ttggggcttt aaccgatgag
     2821 gtgaaacgcc gtggttatca gaaggcgctg atcgtcaccg ataaaacgct ggtgcaatgc
     2881 ggcgtggtgg cgaaagtgac cgataagatg gatgctgcag ggctggcatg ggcgatttac
     2941 gacggcgtag tgcccaaccc aacaattact gtcgtcaaag aagggctcgg tgtattccag
     3001 aatagcggcg cggattacct gatcgctatt ggtggtggtt ctccacagga tacttgtaaa
     3061 gcgattggca ttatcagcaa caacccggag tttgccgatg tgcgtagcct ggaagggctt
     3121 tccccgacca ataaacccag tgtaccgatt ctggcaattc ctaccacagc aggtactgcg
     3181 gcagaagtga ccattaacta cgtgatcact gacgaagaga aacggcgcaa gtttgtttgc
     3241 gttgatccgc atgatatccc gcaggtggcg tttattgacg ctgacatgat ggatggtatg
     3301 cctccagcgc tgaaagctgc gacgggtgtc gatgcgctca ctcatgctat tgaggggtat
     3361 attacccgtg gcgcgtgggc gctaaccgat gcactgcaca ttaaagcgat tgaaatcatt
     3421 gctggggcgc tgcgaggatc ggttgctggt gataaggatg ccggagaaga aatggcgctc
     3481 gggcagtatg ttgcgggtat gggcttctcg aatgttgggt tagggttggt gcatggtatg
     3541 gcgcatccac tgggcgcgtt ttataacact ccacacggtg ttgcgaacgc catcctgtta
     3601 ccgcatgtca tgcgttataa cgctgacttt accggtgaga agtaccgcga tatcgcgcgc
     3661 gttatgggcg tgaaagtgga aggtatgagc ctggaagagg cgcgtaatgc cgctgttgaa
     3721 gcggtgtttg ctctcaaccg tgatgtcggt attccgccac atttgcgtga tgttggtgta
     3781 cgcaaggaag acattccggc actggcgcag gcggcactgg atgatgtttg taccggtggc
     3841 aacccgcgtg aagcaacgct tgaggatatt gtagagcttt accataccgc ctggtaaccg
     3901 ttcatttatc acaaaaggat tgttcgatgt cagtacccgt tcaacatcct atgtatatcg
     3961 atggacagtt tgttacctgg cgtggagacg catggattga tgtggtaaac cctgctacag
     4021 aggctgtcat ttcccgcata cccgatggtc aggccgagga tgcccgtaag gcaatcgatg
     4081 cagcagaacg tgcacaacca gaatgggaag cgttgcctgc tattgaacgc gccagttggt
     4141 tgcgcaaaat ctccgccggg atccgcgaac gcgccagtga aatcagtgcg ctgattgttg
     4201 aagaaggggg caagatccag cagctggctg aagtcgaagt ggcttttact gccgactata
     4261 tcgattacat ggcggagtgg gcacggcgtt acgagggcga gattattcaa agcgatcgtc
     4321 caggagaaaa tattcttttg tttaaacgtg cgcttggtgt gactaccggc attctgccgt
     4381 ggaacttccc gttcttcctc attgcccgca aaatggctcc cgctcttttg accggtaata
     4441 ccatcgtcat taaacctagt gaatttacgc caaacaatgc gattgcattc gccaaaatcg
     4501 tcgatgaaat aggccttccg cgcggcgtgt ttaaccttgt actggggcgt ggtgaaaccg
     4561 ttgggcaaga actggcgggt aacccaaagg tcgcaatggt cagtatgaca ggcagcgtct
     4621 ctgcaggtga gaagatcatg gcgactgcgg cgaaaaacat caccaaagtg tgtctggaat
     4681 tggggggtaa agcaccagct atcgtaatgg acgatgccga tcttgaactg gcagtcaaag
     4741 ccatcgttga ttcacgcgtc attaatagtg ggcaagtgtg taactgtgca gaacgtgttt
     4801 atgtacagaa aggcatttat gatcagttcg tcaatcggct gggtgaagcg atgcaggcgg
     4861 ttcaatttgg taaccccgct gaacgcaacg acattgcgat ggggccgttg attaacgccg
     4921 cggcgctgga aagggtcgag caaaaagtgg cgcgcgcagt agaagaaggg gcgagagtgg
     4981 cgttcggtgg caaagcggta gaggggaaag gatattatta tccgccgaca ttgctgctgg
     5041 atgttcgcca ggaaatgtcg attatgcatg aggaaacctt tggcccggtg ctgccagttg
     5101 tcgcatttga cacgctggaa gatgctatct caatggctaa tgacagtgat tacggcctga
     5161 cctcatcaat ctatacccaa aatctgaacg tcgcgatgaa agccattaaa gggctgaagt
     5221 ttggtgaaac ttacatcaac cgtgaaaact tcgaagctat gcaaggcttc cacgccggat
     5281 ggcgtaaatc cggtattggc ggcgcagatg gtaaacatgg cttgcatgaa tatctgcaga
     5341 cccaggtggt ttatttacag tct
//
