LOCUS       sequence-298299-        1655 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast Expression plasmid for SARS-CoV-2 3C-like proteinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1655)
  TITLE     SARS-CoV-2 protein Yeast expression constructs, tagged, for protein 
            expression and/or purification
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4046684
FEATURES             Location/Qualifiers
     source          1..1655
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(1144..1162)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 gaaaattttt ttttttgatt tttttctctt tcgatgacct cccattgata tttaagttaa
       61 taaacggtct tcaatttctc aagtttcagt ttcatttttc ttgttctatt acaacttttt
      121 ttacttcttg ctcattagaa agaaagcata gcaatctaat ctaagtttta attacaaaat
      181 gtcgggtttc agaaaaatgg cctttcctag tggaaaggtc gagggctgta tggtgcaagt
      241 aacatgcggt acgaccactt tgaacggttt atggcttgac gatgttgttt actgtccacg
      301 tcatgtcatc tgtacttcag aagatatgtt gaatccaaac tatgaagacc tactgattcg
      361 caagtctaac cacaatttct tagttcaggc tggtaacgtt caattgagag ttataggtca
      421 cagcatgcaa aattgtgtct tgaagttaaa agttgatacc gcaaacccga agactccaaa
      481 gtacaaattt gtcagaatcc aacccggtca aaccttctcc gtcttggctt gttataacgg
      541 gtccccatct ggtgtatacc agtgcgctat gaggccaaac ttcactatta agggttcttt
      601 tttgaatggt tcttgtggct ccgttggttt taatatcgac tacgattgcg tcagtttctg
      661 ttacatgcat cacatggaat tgcctacagg tgttcacgcc ggaaccgacc tagaagggaa
      721 cttctatggt ccattcgttg atcggcaaac tgctcaagct gcgggtacgg ataccacaat
      781 tactgtaaat gtgcttgcct ggttgtacgc tgctgttata aacggtgacc gatggttttt
      841 aaacagattc actaccacat tgaatgactt taatctggtc gctatgaaat ataactacga
      901 accactcact caagatcatg tcgatatttt aggtcctttg tctgcacaaa ctggcatcgc
      961 tgttttggac atgtgtgcgt cccttaagga actactccaa aacggtatga acgggagaac
     1021 gattttgggt tcagccctat tggaggacga atttacccca ttcgatgtgg ttagacagtg
     1081 ctctggagta accttccaat aaacaggccc cttttccttt gtcgatatca tgtaattagt
     1141 tatgtcacgc ttacattcac gccctcctcc cacatccgct ctaaccgaaa aggaaggagt
     1201 tagacaacct gaagtctagg tccctattta ttttttttaa tagttatgtt agtattaaga
     1261 acgttattta tatttcaaat ttttcttttt tttctgtaca aagcggccgc cgaaccacta
     1321 gtgcactgca gtacacggtt tccttgaaat ttttttgatt cggtaatctc cgaacagaag
     1381 gaagaacgaa ggaaggagca cagacttaga ttggtatata tacgcatatg tagtgttgaa
     1441 gaaacatgaa attgcccagt attcttaacc caactgcaca gaacaaaaac gtgcaggaaa
     1501 cgaagataaa tcatgtcgaa agctacatat aaggaacgtg ctgctactca tcctagtcct
     1561 gttgctgcca agctatttaa tatcatgcac gaaaagcaaa caaacttgtg tgcttcattg
     1621 gatgttcgta ccaccaagga attactggag ttagt
//
