LOCUS       sequence-298315-        2904 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of ORF10wu gene's CDS 
            from SARS-CoV-2 genome definition in Wu et al, Nature 2020 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR223 SARS-CoV-2 ORF10wu_nostop
FEATURES             Location/Qualifiers
     source          1..2904
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(46..73)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(165..251)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     300..322
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     314..331
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     315..331
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    347..446
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(561..660)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(677..696)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(678..696)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(701..717)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(701..717)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1148..1939
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2032..2620
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2521..2540
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2774..2791
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aattaatacg cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc
       61 aggatggcct tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac
      121 cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt cctactcagg
      181 agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtcttccga ctgagccttt
      241 cgttttattt gatgcctggc agttccctac tctcgcgtta acgctagcat ggatgttttc
      301 ccagtcacga cgttgtaaaa cgacggccag tcttaagctc gggccccaaa taatgatttt
      361 attttgactg atagtgacct gttcgttgca acaaattgat gagcaatgct tttttataat
      421 gccaactttg tacaaaaaag ttggcatggg ctacatcaac gtgttcgcct tccccttcac
      481 catctacagc ctgctgctgt gccgcatgaa ctccaggaac tacatcgctc aggtggacgt
      541 ggtgaacttc aacctgacct acccaacttt cttgtacaaa gttggcatta taagaaagca
      601 ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg
      661 ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca
      721 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
      781 atgcctcctc tagaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc
      841 cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc
      901 ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac
      961 cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt
     1021 tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg
     1081 tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt
     1141 aaacattatg agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg
     1201 cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt
     1261 ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct
     1321 tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg
     1381 agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc
     1441 gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct
     1501 tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc
     1561 aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc
     1621 tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga
     1681 ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt
     1741 aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc
     1801 ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg
     1861 cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac
     1921 caaggtagtc ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta
     1981 cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     2041 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     2101 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     2161 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     2221 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     2281 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     2341 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     2401 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     2461 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     2521 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     2581 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     2641 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     2701 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     2761 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     2821 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     2881 gaaagcgggc agtgagcgca acgc
//
